Detailed information of component protein


Summary

Component ID T6CP010937
Component protein type TssH
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (895 a.a.); Nucleotide sequence (2685 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0022
Coordinate (Strand) 28396..31080 (+)
NCBI ID 225382165
GenBank FJ790776
Uniprot ID C0LZP0_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.8e-44 628..792
AAA_lid_9 PF17871.3 6.4e-31 359..454
ClpB_D2-small PF10431.11 1.4e-13 799..873
AAA PF00004.31 7.5e-10 222..350
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-894 MAMININLSSLIQRLHPIAKVALEDAAALAVSEKANEVQIEHYLLSLLERPNSDFDVLLSHFDCSENLLRQSVRSTLDTSAKGNGSKPVFSALLIEWLQESWLVSSLDLSETQIRSGALLLTLVSNPLRYGQHGYASILEAVNPDSLKRNFAELTSQSIEAQVATSEKTQAREDGSALSKFTTDFTGKARKGEIDPVFCRDQEIRQIVDILARRRKNNPIAVGEPGVGKTAVVEGLALKIVQGDVPDNLKGVELYGLDMGLLQAGASVKGEFEKRLNAVLDEVKNSPTPIILFIDEAHTLVGGGNQAGGSDAANLLKPALARGEVKTIAATTWSEYKKYFEKDPALARRFQLVKLDEPSPEQAALIIRGLRPAYEKSHNVYVRDDAITAAAALSARYISGRQLPDKAIDVLDTACARVNISLNAIPASVETLQQELAAQQRELEALERDALQQTGDKHSLANIPDLKLAMETTKEELAEQEAQWHKEKEQIQEMIALRSRLHELVFGETNEEELVAEEDSEQEQNVSPYAEMDEETVRIAISACQEQLDVIRNGNPLVHFEVGPDEVSHVISDWTGIPMGKMLQDEAETTLKLKESLTQSIKGQEYAIDALSEGIQTAKAGLSNPDAPTGVFLLVGPSGVGKTETARAIADQMFGGERFMTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKADPEVLNLFYQVFDKGTLNDGEGRTIDFKNTLIIMTSNLATHEIESLVHQSKDIDANIIAEAIRPTLNQHFKPALLARMSVLPFVPLSDEAMTEIIHHKLNKVSQRLQSHHKLSLSYEESLVEFVLGNCRLAETGARNIDAVINRQLLPQLSTQLLVHDKDDSHTQITVSVDEQGTLTYAFS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9924 - -


  Protein sequence: 895 a.a.    

>T6CP010937 FJ790776:28396-31080 [Vibrio alginolyticus] [TssH]
MAMININLSSLIQRLHPIAKVALEDAAALAVSEKANEVQIEHYLLSLLERPNSDFDVLLSHFDCSENLLRQSVRSTLDTS
AKGNGSKPVFSALLIEWLQESWLVSSLDLSETQIRSGALLLTLVSNPLRYGQHGYASILEAVNPDSLKRNFAELTSQSIE
AQVATSEKTQAREDGSALSKFTTDFTGKARKGEIDPVFCRDQEIRQIVDILARRRKNNPIAVGEPGVGKTAVVEGLALKI
VQGDVPDNLKGVELYGLDMGLLQAGASVKGEFEKRLNAVLDEVKNSPTPIILFIDEAHTLVGGGNQAGGSDAANLLKPAL
ARGEVKTIAATTWSEYKKYFEKDPALARRFQLVKLDEPSPEQAALIIRGLRPAYEKSHNVYVRDDAITAAAALSARYISG
RQLPDKAIDVLDTACARVNISLNAIPASVETLQQELAAQQRELEALERDALQQTGDKHSLANIPDLKLAMETTKEELAEQ
EAQWHKEKEQIQEMIALRSRLHELVFGETNEEELVAEEDSEQEQNVSPYAEMDEETVRIAISACQEQLDVIRNGNPLVHF
EVGPDEVSHVISDWTGIPMGKMLQDEAETTLKLKESLTQSIKGQEYAIDALSEGIQTAKAGLSNPDAPTGVFLLVGPSGV
GKTETARAIADQMFGGERFMTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKADPEVLNL
FYQVFDKGTLNDGEGRTIDFKNTLIIMTSNLATHEIESLVHQSKDIDANIIAEAIRPTLNQHFKPALLARMSVLPFVPLS
DEAMTEIIHHKLNKVSQRLQSHHKLSLSYEESLVEFVLGNCRLAETGARNIDAVINRQLLPQLSTQLLVHDKDDSHTQIT
VSVDEQGTLTYAFS*

  Nucleotide sequence: 2685 bp    

>T6CP010937 FJ790776:28396-31080 [Vibrio alginolyticus] [TssH]
ATGGCTATGATAAATATAAACCTTTCTTCATTAATTCAGCGTCTACACCCGATTGCAAAAGTAGCATTAGAAGACGCCGC
TGCACTTGCAGTCTCTGAAAAAGCAAACGAAGTTCAAATTGAGCATTATTTACTGAGTCTATTAGAAAGACCAAATAGTG
ATTTTGACGTCTTATTAAGTCATTTTGATTGTTCTGAGAACTTATTAAGACAATCGGTTCGATCCACACTCGACACCAGC
GCCAAAGGAAATGGCAGCAAACCTGTTTTTTCTGCGTTATTAATCGAATGGTTACAAGAAAGCTGGCTGGTTTCTTCTTT
AGATCTATCCGAAACACAAATCCGCTCTGGTGCCCTACTCCTTACTCTTGTTAGCAACCCTCTCCGTTACGGCCAACATG
GTTACGCCTCTATCCTTGAAGCGGTAAACCCTGACAGCCTTAAGCGTAACTTTGCAGAGCTAACAAGCCAATCTATTGAG
GCGCAAGTTGCTACTTCAGAGAAAACACAAGCTCGTGAGGATGGCTCAGCACTGAGCAAGTTCACAACAGACTTCACTGG
TAAAGCACGCAAAGGCGAAATCGATCCTGTTTTCTGCCGCGACCAAGAAATCCGCCAAATCGTGGACATTCTTGCTCGTC
GCCGTAAAAACAACCCTATTGCTGTTGGTGAACCGGGCGTTGGTAAAACCGCAGTAGTAGAAGGCTTGGCACTTAAGATT
GTACAAGGTGACGTACCAGACAACCTCAAAGGTGTTGAGCTTTACGGGCTAGATATGGGTCTTCTGCAAGCTGGTGCAAG
CGTAAAAGGTGAATTCGAAAAACGCCTAAACGCGGTATTGGATGAAGTGAAAAACTCGCCAACCCCTATCATTCTTTTCA
TCGATGAAGCACACACCCTTGTTGGCGGTGGTAACCAAGCGGGCGGCAGCGACGCGGCAAACTTGCTAAAACCAGCCCTA
GCGCGTGGTGAAGTGAAAACCATCGCCGCAACAACATGGTCGGAATACAAGAAGTACTTCGAGAAAGATCCTGCCCTTGC
ACGTCGTTTCCAATTGGTGAAACTCGACGAACCGTCTCCTGAGCAAGCAGCATTGATCATTCGTGGTCTGCGCCCAGCAT
ACGAGAAGTCACACAACGTTTACGTGCGTGACGATGCTATCACTGCTGCGGCAGCACTTTCTGCTCGTTACATCTCTGGT
CGTCAACTGCCAGACAAAGCGATCGATGTACTAGATACGGCGTGTGCACGAGTGAACATCAGCCTTAATGCCATTCCTGC
TTCTGTTGAGACTCTGCAACAAGAACTTGCAGCTCAACAGCGCGAGCTTGAAGCACTTGAGCGTGATGCTCTGCAGCAAA
CTGGCGACAAGCACAGCCTTGCTAACATTCCAGACCTAAAACTGGCAATGGAAACAACGAAAGAAGAGCTAGCAGAACAA
GAAGCGCAATGGCACAAAGAAAAAGAGCAGATCCAAGAGATGATTGCTCTACGCTCTCGTTTGCATGAACTGGTCTTTGG
CGAAACAAATGAAGAGGAGCTTGTTGCAGAAGAAGATTCAGAACAAGAGCAAAACGTTTCTCCATATGCAGAAATGGATG
AAGAAACCGTTCGCATTGCAATTTCGGCCTGCCAAGAGCAGCTAGATGTAATCCGCAATGGCAACCCACTGGTTCACTTT
GAAGTGGGTCCAGATGAAGTTAGCCACGTCATCTCGGACTGGACTGGTATTCCTATGGGTAAGATGCTTCAAGACGAAGC
AGAAACCACGCTAAAACTGAAAGAAAGCCTAACACAAAGCATCAAAGGCCAAGAATACGCTATCGATGCACTGTCTGAAG
GCATTCAAACTGCGAAAGCTGGTCTAAGTAACCCAGACGCACCAACAGGCGTATTCCTGCTGGTTGGCCCAAGTGGCGTG
GGTAAAACCGAAACGGCCCGTGCGATCGCAGATCAAATGTTTGGTGGTGAGCGCTTTATGACGACCATTAACATGTCTGA
GTTCCAAGAGAAGCACACGGTATCACGCCTAATTGGTTCACCTCCGGGTTACGTAGGTTACGGTGAAGGCGGCATGCTGA
CAGAAGCGGTTCGTCAACGTCCATACTCTGTTGTTCTTCTTGATGAAGTGGAAAAAGCCGACCCTGAAGTATTGAACCTG
TTCTACCAAGTGTTCGACAAAGGCACGCTGAACGACGGCGAAGGCCGCACAATCGACTTCAAGAACACGCTGATCATCAT
GACCAGTAACCTTGCGACGCATGAGATCGAATCTTTGGTTCATCAATCAAAAGACATCGATGCGAACATCATCGCAGAAG
CTATTCGCCCTACTTTGAACCAACACTTCAAGCCGGCGCTGTTGGCACGTATGTCAGTACTACCATTTGTTCCGCTGTCT
GATGAAGCAATGACAGAAATTATTCACCACAAGCTGAACAAAGTTTCTCAGCGTCTACAGAGCCATCATAAATTGTCTCT
GAGCTACGAAGAAAGTTTGGTTGAGTTCGTACTAGGTAACTGTCGCCTTGCTGAAACGGGCGCGCGTAACATCGACGCGG
TCATCAACCGTCAGTTACTGCCTCAGCTATCGACACAGCTATTGGTGCATGATAAGGATGATTCACACACGCAAATCACA
GTTTCTGTCGATGAGCAAGGAACACTGACCTATGCGTTCAGCTAA