Detailed information of component protein


Summary

Component ID T6CP010925
Component protein type TssL
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (264 a.a.); Nucleotide sequence (792 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0002
Coordinate (Strand) 1679..2470 (-)
NCBI ID 225382145
GenBank FJ790776
Uniprot ID C0LZM0_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.4e-50 47..246
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-230 MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLSIDNMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAREIRKGERKLNEMELDRASVLVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFQGKYGREERGNEKLAEIGSTIYRLVRDDRLAEQEKVSLINLKAKYLKKPLKRVISPK
Transmembrane helix 231-253 LILSLSAMTFALMYAATYIVIDL
Outside 254-263 KFQQLLEMYR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9950 - -


  Protein sequence: 264 a.a.    

>T6CP010925 FJ790776:c2470-1679 [Vibrio alginolyticus] [TssL]
MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLSIDNMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERKLNEMELDRASVLVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRH
VIEFLYLLLQLGFQGKYGREERGNEKLAEIGSTIYRLVRDDRLAEQEKVSLINLKAKYLKKPLKRVISPKLILSLSAMTF
ALMYAATYIVIDLKFQQLLEMYR*

  Nucleotide sequence: 792 bp    

>T6CP010925 FJ790776:c2470-1679 [Vibrio alginolyticus] [TssL]
ATGAGTTACGCAGAAACCGACGTCACCGTAGTCTTATTCCAACCAGAGCCTGGCAAACCAATGGAGGTAATGCCAGCGCC
TGACTTGTCGAGAAACATCGCAGTACAAGATCTATCTATCGACAACATGGGTATTAACCCGCTAGTGGACCAGTTCTCAT
GGTTGATCGCCAGTCTGTCTTGTATGTCTTCGATCCCTTGGCTTGATGACCCGATGCCATTTCGTGAACAAGTGGCACGT
GAGATTCGAAAAGGTGAACGAAAACTTAATGAAATGGAGCTCGATCGTGCTTCGGTTCTTGTTATTCGTTACTGCTTGTG
TGCGGCGATTGATGAATCTGTTTGTCGACAAGAGTGGGGGGCAAATAGTCACTGGAGCCAGAACAGCTTACTGTCTGAAT
TCCACAACGAGACATCTGGTGGTGACAAGTTTTTCGTTATTCTCGACCGCTTAAAAGCCGACCCTCGCAAATACCGTCAC
GTTATAGAGTTTCTCTATTTATTGCTTCAACTTGGTTTCCAAGGCAAGTACGGCCGTGAAGAGCGTGGCAACGAAAAGCT
AGCGGAAATTGGCAGCACCATTTATCGATTAGTGCGAGATGACCGCCTAGCAGAACAAGAAAAAGTTTCTTTAATCAACC
TTAAAGCTAAATATCTAAAGAAGCCGTTAAAAAGGGTAATTTCACCCAAGCTGATCTTAAGTCTTAGTGCGATGACGTTT
GCGCTTATGTATGCGGCAACCTACATCGTCATAGACTTAAAATTTCAGCAACTGCTTGAAATGTATAGATAA