Detailed information of component protein


Summary

Component ID T6CP010930
Component protein type TssF
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (512 a.a.); Nucleotide sequence (1536 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0010
Coordinate (Strand) 13544..15079 (-)
NCBI ID 225382153
GenBank FJ790776
Uniprot ID C0LZM8_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 5.8e-133 5..467
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-511 MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTSPRPHYMRSIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSMIDVTLSTEHGLELSRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYFELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQEFFSLPDKFMFFDIKGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSNTQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVLMKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLATIDVVERVALPLTTSPLQTDRKATRAYPTCAQLFFAQYGCVKVVL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9959 - -


  Protein sequence: 512 a.a.    

>T6CP010930 FJ790776:c15079-13544 [Vibrio alginolyticus] [TssF]
MSNSKYFQDELTYLRESGSEFAKYHPKLTHFLSEGTFDPDVERLLEGFAFLTGRIREKIDDELPELTQSLMTSPRPHYMR
SIPSLCISELKPHTGSVTEKTVVKRGAEMASEQVEGTQCLFRTCYDVNLYPITITNIEQTNSRTSSMIDVTLSTEHGLEL
SRIGLDTLRLHLHGEIHITRTVFLWLFRYLDYFELDVGGGYKHRLGPEYVKPVGHEEDEALLPYSKNSFAGYRLLQEFFS
LPDKFMFFDIKGLEWLKGIPQRSTVKIKFHFKRALPSEVVLKDKHLRLHCTPAVNLFEKDGDPIRLEHRRNEYKVRPQSN
TQEHYEVYSIEQVESWSKDERRRKPLIEFESFEHQINQRDKREFYKSKVGERVSGRGLERYISFHTHNGDIADLGTETVL
MKLQCSNADLAERLSVGDITYATHKSPTYATFKNITKPTQSVSPQVNGELQWQLIANMSLNYLSLATIDVVERVALPLTT
SPLQTDRKATRAYPTCAQLFFAQYGCVKVVL*

  Nucleotide sequence: 1536 bp    

>T6CP010930 FJ790776:c15079-13544 [Vibrio alginolyticus] [TssF]
TTGAGTAACAGTAAATACTTTCAAGATGAACTCACGTATTTACGCGAGTCGGGTAGTGAGTTTGCCAAATATCATCCGAA
GTTAACTCACTTTCTCTCTGAAGGAACTTTTGATCCAGATGTCGAACGATTGCTGGAAGGTTTTGCTTTTTTGACCGGAC
GCATCCGCGAAAAGATTGACGATGAACTTCCTGAGCTAACGCAATCTCTGATGACTTCCCCGCGCCCACATTACATGCGT
TCGATCCCGTCTTTGTGCATTAGCGAACTAAAACCGCATACCGGAAGTGTTACTGAGAAAACCGTGGTGAAACGTGGTGC
GGAAATGGCTAGCGAGCAGGTAGAAGGCACGCAGTGTTTGTTCCGTACTTGTTATGACGTCAATCTATACCCAATTACGA
TTACTAACATTGAACAAACTAACAGCCGCACCAGTTCGATGATTGATGTAACGTTATCGACGGAGCATGGCCTTGAGCTG
TCTCGGATAGGTTTAGACACGCTGCGATTGCACCTGCATGGTGAGATTCATATTACTCGCACTGTCTTCTTATGGCTGTT
CCGTTATCTAGATTACTTCGAGTTGGATGTTGGTGGCGGCTACAAACATCGTTTAGGGCCAGAATACGTTAAGCCAGTCG
GGCATGAAGAGGACGAGGCGCTGTTGCCCTACAGCAAAAACTCCTTTGCAGGCTACCGTTTGCTGCAAGAATTCTTCTCT
TTGCCAGATAAGTTCATGTTCTTCGACATTAAAGGCTTAGAGTGGCTAAAAGGCATTCCACAGCGCAGTACGGTCAAAAT
CAAATTCCACTTTAAGCGTGCTTTGCCTTCAGAGGTGGTGCTTAAAGATAAACACTTAAGGTTGCACTGCACACCTGCCG
TTAACTTGTTTGAAAAAGACGGCGATCCAATCCGTTTAGAGCATCGACGAAACGAGTACAAGGTGCGTCCACAAAGTAAC
ACTCAGGAGCATTACGAGGTTTATTCCATTGAGCAAGTGGAGAGCTGGAGTAAAGACGAGCGTCGCCGAAAACCACTGAT
TGAATTTGAATCGTTTGAGCACCAAATCAACCAGCGTGATAAGCGAGAGTTCTACAAATCCAAAGTGGGCGAGCGAGTAA
GTGGGAGAGGGTTAGAGCGCTATATCTCATTCCATACTCACAATGGTGACATTGCGGATTTAGGGACTGAAACAGTATTG
ATGAAACTGCAATGCAGTAACGCAGACTTAGCGGAGCGCCTGTCAGTAGGAGATATCACGTACGCGACGCACAAATCGCC
AACGTATGCGACGTTTAAAAACATTACTAAGCCAACACAGTCAGTAAGTCCTCAGGTTAATGGTGAACTTCAATGGCAGT
TGATTGCCAACATGTCGCTCAACTACTTGTCTTTGGCCACTATCGATGTGGTTGAAAGAGTTGCTCTTCCACTTACGACT
TCCCCTCTCCAGACCGATAGAAAAGCAACGCGCGCATACCCAACATGCGCTCAATTATTTTTTGCCCAATATGGATGTGT
GAAAGTTGTGCTCTGA