Detailed information of component protein


Summary

Component ID T6CP010927
Component protein type TssA
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (438 a.a.); Nucleotide sequence (1314 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0006
Coordinate (Strand) 6951..8264 (+)
NCBI ID 225382149
GenBank FJ790776
Uniprot ID C0LZM4_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 7.4e-25 9..119
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-236 MFFTDFARRPIDENNPSGSNPNGSEDFDEIKRQINNLNKVTGRVSWKTVQTLSKKILSTQAKDLRCCCYFTVASTYNDGLRGLVEGLNSILDVCVVYWFNAYPEHSKSKARMSAIEWMVEHTEKRINKHRALPEELPLIEAAHQLCLRIEEELRLHYGIEAPSLGRIRRILNQWIEQIKEQQIAAELREKKQANTSAPAATQKAPEIKVNVSPSPAPVSSPNLNSQEKASPAKSGR
Transmembrane helix 237-256 FMIYFVIGLIVTAICSHFMY
Inside 257-437 EQQRYRTLEQKINQASLNELVNLVASLDYENKKYAQSLKTQAINRVDIMMNNWFLDSAKVSNVSELDSLTNELIAIYPDSSSAQQLRQNFLTQSAHLEDQFMDVYKRFSDARTVFANVARQNVDKNSEKAYEYSNSLFPLLGRIEYAEKYSQQKEIDRATLLLNIYLYKLSQLQAEKTDNK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9382 - -


  Protein sequence: 438 a.a.    

>T6CP010927 FJ790776:6951-8264 [Vibrio alginolyticus] [TssA]
MFFTDFARRPIDENNPSGSNPNGSEDFDEIKRQINNLNKVTGRVSWKTVQTLSKKILSTQAKDLRCCCYFTVASTYNDGL
RGLVEGLNSILDVCVVYWFNAYPEHSKSKARMSAIEWMVEHTEKRINKHRALPEELPLIEAAHQLCLRIEEELRLHYGIE
APSLGRIRRILNQWIEQIKEQQIAAELREKKQANTSAPAATQKAPEIKVNVSPSPAPVSSPNLNSQEKASPAKSGRFMIY
FVIGLIVTAICSHFMYEQQRYRTLEQKINQASLNELVNLVASLDYENKKYAQSLKTQAINRVDIMMNNWFLDSAKVSNVS
ELDSLTNELIAIYPDSSSAQQLRQNFLTQSAHLEDQFMDVYKRFSDARTVFANVARQNVDKNSEKAYEYSNSLFPLLGRI
EYAEKYSQQKEIDRATLLLNIYLYKLSQLQAEKTDNK*

  Nucleotide sequence: 1314 bp    

>T6CP010927 FJ790776:6951-8264 [Vibrio alginolyticus] [TssA]
ATGTTTTTTACCGACTTCGCTCGAAGACCAATCGATGAAAATAACCCAAGCGGCTCTAATCCTAATGGTTCTGAGGATTT
TGACGAAATTAAACGTCAAATAAACAACCTGAATAAAGTCACAGGGAGAGTCTCGTGGAAAACGGTTCAAACACTCTCAA
AAAAGATCTTGAGTACCCAAGCGAAAGACCTACGTTGTTGCTGTTACTTTACCGTTGCGTCAACCTATAACGACGGCTTA
AGAGGCCTTGTTGAAGGGTTAAACTCAATACTTGATGTGTGTGTCGTATATTGGTTCAACGCGTATCCAGAGCATTCAAA
GTCAAAGGCACGAATGAGCGCTATTGAATGGATGGTAGAGCATACTGAAAAACGAATTAATAAGCATCGGGCGTTACCAG
AAGAGCTTCCATTGATAGAGGCAGCACATCAATTATGCTTGAGGATTGAGGAAGAGCTTCGTCTTCATTACGGTATTGAA
GCGCCGTCATTAGGTCGCATTCGCCGTATTTTGAATCAATGGATTGAGCAGATAAAAGAACAACAAATTGCGGCTGAGTT
ACGCGAGAAAAAACAAGCCAACACTAGCGCTCCTGCAGCAACGCAGAAGGCACCTGAAATTAAAGTGAATGTGTCTCCCT
CTCCCGCACCGGTGTCATCACCCAACCTAAATAGCCAAGAGAAAGCATCACCGGCAAAGTCTGGTCGATTTATGATCTAC
TTCGTTATTGGTTTGATTGTTACTGCGATATGTTCACATTTCATGTACGAGCAACAACGTTATCGAACATTGGAGCAAAA
AATTAATCAAGCTTCACTTAATGAACTTGTAAATTTGGTCGCCTCGTTAGATTACGAAAATAAAAAATATGCTCAGTCAT
TGAAAACGCAGGCCATTAATCGTGTCGATATTATGATGAATAACTGGTTTTTAGACTCGGCAAAAGTAAGTAACGTCAGC
GAGCTCGATAGCTTGACCAATGAATTAATCGCAATCTATCCGGATTCGTCTTCTGCACAGCAGCTACGTCAAAACTTTTT
GACACAGAGCGCCCATTTAGAAGACCAGTTTATGGATGTTTATAAGCGATTCTCAGACGCAAGAACGGTGTTTGCCAACG
TAGCTAGACAGAATGTAGATAAGAACTCTGAAAAGGCGTACGAATACAGTAACTCGTTATTCCCGCTTTTAGGTCGTATT
GAATACGCTGAAAAGTATTCACAGCAAAAGGAAATTGACCGCGCAACCCTTTTACTGAACATCTACCTATATAAATTGAG
TCAGCTACAAGCTGAAAAAACTGACAATAAATAA