Detailed information of regulator


Summary

Regulator ID REG0376 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (798 a.a.); Nucleotide sequence (2394 bp)
Description The knockdown of bamA and lptD led to increased transcription of H1-T6SS genes. Sains expressing sg-bamA, sg-lptD, and sg-tolB all exhibited increased ClpV-GFP foci.
Reference
External database links

Locus tag (Gene) PA3648 (bamA)
Coordinates (Strand) 4085062..4087455 (-)
NCBI ID NP_252338.1
RefSeq NC_002516
Uniprot ID Q9HXY4_PSEAE
KEGG ID pae:PA3648
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
ClpV-GFP foci accumulate; H1-T6SS transcript ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Omp85 PF01103.25 1.1e-96 447..797
POTRA PF07244.17 7.7e-17 175..262
POTRA PF07244.17 1e-15 347..419
POTRA PF07244.17 4.3e-15 265..343
POTRA PF07244.17 2.4e-13 92..171
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-797 MKRFLLPALLSALMIAEVHAESFTVSDIRVNGLQRVSAGSVFAALPLNVGETIDDQALVQATRSLFKTGFFQDIQLGRDGNVLVVTVVERPSISSIEIEGNKAISKEDLLKGLKQSGLAEGEIFQRATLEGVRNELQRQYVAQGRYSAEINAEVIPQPRNRVALKININEGTVAAISHINVVGNTVFSEEDLTDLFELKTTNWLSFFKNDDKYAREKLSGDLERLRSYYLDRGYINMDIASTQVSITPDKKHVYITVNINEGEKYTIRDVKLTGDLKVPEEEVKRLLLVQKGQVFSRKVMTTTSDLITRRLGNEGYTFANVNGVPEAHDDDKTVSVTFVVDPGKRAYVNRINFRGNTKTEDEVLRREMRQMEGGWASTYLIDQSKARLERLGYFKEVNVETPAVPGTDDQVDVNYSVEEQPSGSITASVGFAQSAGLILGGSISQNNFLGTGNKVSIGLTRSEYQTRYNFGFVDPYWTVDGVSLGYNAFYRKTDYDELDVDVASYSVNSLGAGMSIGYPISETSRLTYGLSVQRDQIDTGRYTVDEIYDFLDKEGDNFTNFKASIGWSESTLNKGVLATRGHSQSLTLETTLPGSDLSFYKIDYRGQVFAPLTDNYTMRFHTELGYGDGYGSTERLPFYENYYAGGFNSVRGFKDSTLGPRSTPSVARNPDGTPMKNQGPDSKGRYTDPDQDPEAFGGNILITGGAELLFPLPFVKDQRQLRTVLFWDVGSTFDTDCPTKTTTNCDGIKTDNLASSVGVGLTWITALGPLSFSLATPIKKPDNAETQVFQFSLGQTF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9964 pos: 20-21. VHA-ES. MKRFLLPALLSALMIAEVHA


  Protein sequence of regulator: 798 a.a.    .

>REG0376 NC_002516:c4087455-4085062 [Pseudomonas aeruginosa PAO1]
MKRFLLPALLSALMIAEVHAESFTVSDIRVNGLQRVSAGSVFAALPLNVGETIDDQALVQATRSLFKTGFFQDIQLGRDG
NVLVVTVVERPSISSIEIEGNKAISKEDLLKGLKQSGLAEGEIFQRATLEGVRNELQRQYVAQGRYSAEINAEVIPQPRN
RVALKININEGTVAAISHINVVGNTVFSEEDLTDLFELKTTNWLSFFKNDDKYAREKLSGDLERLRSYYLDRGYINMDIA
STQVSITPDKKHVYITVNINEGEKYTIRDVKLTGDLKVPEEEVKRLLLVQKGQVFSRKVMTTTSDLITRRLGNEGYTFAN
VNGVPEAHDDDKTVSVTFVVDPGKRAYVNRINFRGNTKTEDEVLRREMRQMEGGWASTYLIDQSKARLERLGYFKEVNVE
TPAVPGTDDQVDVNYSVEEQPSGSITASVGFAQSAGLILGGSISQNNFLGTGNKVSIGLTRSEYQTRYNFGFVDPYWTVD
GVSLGYNAFYRKTDYDELDVDVASYSVNSLGAGMSIGYPISETSRLTYGLSVQRDQIDTGRYTVDEIYDFLDKEGDNFTN
FKASIGWSESTLNKGVLATRGHSQSLTLETTLPGSDLSFYKIDYRGQVFAPLTDNYTMRFHTELGYGDGYGSTERLPFYE
NYYAGGFNSVRGFKDSTLGPRSTPSVARNPDGTPMKNQGPDSKGRYTDPDQDPEAFGGNILITGGAELLFPLPFVKDQRQ
LRTVLFWDVGSTFDTDCPTKTTTNCDGIKTDNLASSVGVGLTWITALGPLSFSLATPIKKPDNAETQVFQFSLGQTF*

  Nucleotide sequence of regulator: 2394 bp    .

>REG0376 NC_002516:c4087455-4085062 [Pseudomonas aeruginosa PAO1]
ATGAAACGCTTTCTGCTACCCGCGCTGCTGTCAGCGCTGATGATCGCCGAGGTTCACGCCGAGTCCTTCACTGTTTCCGA
TATCCGGGTCAATGGCCTGCAGCGCGTGTCCGCCGGTAGCGTGTTCGCCGCCCTGCCGCTGAACGTCGGCGAGACCATCG
ACGACCAGGCCCTGGTCCAGGCCACCCGCTCCCTGTTCAAGACCGGCTTCTTCCAGGACATCCAGCTCGGCCGTGATGGC
AACGTGCTGGTAGTCACTGTAGTCGAGCGCCCGTCGATCTCCAGCATCGAGATCGAGGGCAACAAGGCGATCTCCAAGGA
AGACCTGCTCAAGGGCCTGAAACAGTCCGGCCTGGCCGAGGGCGAGATCTTCCAGCGCGCCACCCTCGAAGGCGTGCGCA
ACGAGCTGCAACGCCAGTACGTGGCCCAGGGCCGCTACTCGGCCGAGATCAACGCGGAAGTGATCCCGCAGCCGCGCAAC
CGCGTCGCGCTGAAGATCAACATCAACGAAGGCACGGTCGCCGCGATTTCCCACATCAACGTGGTGGGCAACACCGTCTT
CTCCGAAGAAGACCTGACCGACCTGTTCGAACTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGTACG
CCCGCGAAAAGCTCTCCGGCGACCTCGAGCGCCTGCGCTCCTATTACCTGGACCGCGGCTACATCAACATGGATATCGCC
TCCACCCAGGTATCCATCACCCCGGACAAGAAGCACGTCTACATCACCGTCAACATCAACGAGGGTGAGAAGTACACCAT
CCGCGACGTGAAGCTGACCGGCGACCTGAAGGTGCCGGAAGAGGAAGTGAAGCGCCTGCTGCTGGTACAGAAGGGCCAGG
TGTTCTCGCGCAAGGTGATGACCACCACCTCCGACCTAATCACCCGTCGCCTGGGCAACGAGGGCTACACCTTCGCCAAC
GTCAACGGCGTGCCGGAAGCCCATGACGACGACAAGACCGTCTCGGTGACCTTCGTCGTCGACCCGGGCAAGCGCGCCTA
CGTCAACCGCATCAACTTCCGCGGCAACACCAAGACCGAAGACGAAGTGCTGCGCCGCGAAATGCGCCAGATGGAAGGCG
GCTGGGCCTCCACCTACCTGATCGACCAGTCCAAGGCCCGTCTGGAGCGCCTCGGCTACTTCAAGGAAGTCAACGTCGAG
ACCCCGGCGGTTCCCGGCACCGACGACCAGGTCGACGTCAACTACAGCGTCGAAGAGCAACCGTCCGGCTCGATCACCGC
CAGCGTCGGCTTCGCCCAGAGCGCCGGCCTGATCCTCGGCGGCTCGATCAGCCAGAACAACTTCCTGGGTACCGGCAACA
AGGTCAGCATCGGCCTGACCCGCAGCGAATACCAAACCCGCTACAACTTCGGCTTCGTCGACCCCTACTGGACCGTCGAC
GGCGTCAGCCTCGGCTACAATGCCTTCTACCGGAAGACCGACTACGACGAACTCGACGTCGACGTCGCCAGCTACTCGGT
GAACAGCCTGGGCGCCGGCATGAGCATCGGCTACCCGATCAGCGAGACCTCGCGCCTGACCTATGGCCTGAGCGTGCAGC
GCGACCAGATCGACACCGGCCGCTACACCGTGGACGAGATCTACGACTTCCTCGACAAGGAAGGCGACAACTTCACCAAC
TTCAAGGCTTCCATCGGCTGGTCCGAATCGACCCTGAACAAGGGCGTACTGGCCACCCGTGGTCACTCGCAGAGCCTGAC
CCTGGAAACCACCCTGCCGGGCAGCGACCTGTCGTTCTACAAGATCGACTACCGTGGCCAGGTCTTCGCCCCGCTGACCG
ACAACTACACCATGCGCTTCCACACCGAGCTGGGCTATGGCGACGGCTACGGATCCACCGAGCGCCTGCCGTTCTACGAG
AACTACTACGCCGGCGGCTTCAACTCGGTGCGGGGCTTCAAGGACAGCACGCTGGGCCCGCGCAGTACGCCCTCTGTGGC
TCGTAATCCAGACGGTACGCCTATGAAGAACCAAGGGCCTGACAGCAAGGGCCGTTACACCGACCCGGACCAGGATCCCG
AAGCCTTCGGTGGTAACATCCTGATCACCGGCGGCGCCGAGCTGCTGTTCCCGCTGCCGTTCGTCAAGGACCAGCGCCAG
TTGCGTACCGTGCTGTTCTGGGACGTGGGTAGCACCTTCGATACCGACTGCCCGACCAAGACCACCACCAACTGCGACGG
CATCAAGACCGACAACCTCGCCAGTTCCGTGGGCGTCGGCCTGACCTGGATCACCGCGCTGGGCCCGCTGAGCTTCAGCC
TGGCGACGCCGATCAAGAAGCCGGACAACGCGGAAACCCAGGTCTTCCAGTTCTCCCTGGGCCAGACCTTCTGA