Detailed information of regulator


Summary

Regulator ID REG0377 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (925 a.a.); Nucleotide sequence (2775 bp)
Description The knockdown of bamA and lptD led to increased transcription of H1-T6SS genes. Sains expressing sg-bamA, sg-lptD, and sg-tolB all exhibited increased ClpV-GFP foci.
Reference
External database links

Locus tag (Gene) PA0595 (lptD)
Coordinates (Strand) 653753..656527 (-)
NCBI ID NP_249286.1
RefSeq NC_002516
Uniprot ID LPTD_PSEAE
KEGG ID pae:PA0595
KEGG pathway (determined by BlastKOALA) -
PDB ID 5IVA
  Target
Target Regulation (↓/↑) Target sequence
ClpV-GFP foci accumulate; H1-T6SS transcript ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
LptD PF04453.16 2.4e-120 403..831
LptD_N PF03968.16 4.1e-10 158..291
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-924 MAVKSLVFRRKFPLLVTGSLLALQPVAALTVQAADQFDCKVSATGGWDCSPLQNANANLPPRPAHTATSVSTAAAGSSVSGSGGETVEAEPTQRLVTESGGRALKSRSADYSHLDWIPREKLTAAQLAEIGPYCGGSYIEPVRPGMDDGAPSDESPTYVSAKASRYEQEKQIATLAGDVVLRQGSMQVEGDEANLHQLENRGELVGNVKLRDKGMLVVGDHAQVQLDNGEAQVDNAEYVIHKAHARGSALYAKRSENAIIMLKDGTYTRCEPSSNAWTLKGNNVKLNPATGFGTATNATLRVKDFPVFYTPYIYFPIDDRRQSGFLPPSFSSTSDTGFTLVTPYYFNLAPNYDATLYPRYMAKRGMMLEGEFRYLTHSSEGIVNAAYLNDKDDHREGFPDYSKDRWLYGLKNTTGLDSRWLAEVDYTRISDPYYFQDLDTDLGVGSTTYVNQRGTLTYRGDTFTGRLNAQAYQLATTTDVTPYDRLPQITFDGFLPYNPGGMQFTYGTEFVRFDRDLDENIYFNDDGSIRGKRPDASLQGLARATGDRMHLEPGMSLPMTRSWGYVTPTLKYLYTKYDLDLDSQGKTDLNKRDESFDSNQDRSLPLVKVDSGLYFDRDTTFAGTPFRQTLEPRAMYLYVPYKDQDSLPVFDTSEPSFSYDSLWRENRFTGKDRIGDANQLSLGVTSRFIEENGFERASISAGQIYYFRDRRVQLPGLTEKDLKRLNLDPSGLDNDSWRSPYAFAGQYRFNRDWRINSDFNWNPNTSRTESGSAIFHYQPEVDPGKVVNVGYRYRADARRFDSSRGTFRYGNENDIIKQHDFSVIWPLVPQWSVLARWQYDYNKNRTLEAFGGFEYDSCCWKLRLINRYWLDVDDDAFLVQSEKADRGIFLQIVLKGLGGIVGNKTEMFLDKGIQGYRQREDQAM


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.7494 pos: 33-34. VQA-AD. MAVKSLVFRRKFPLLVTGSLLALQPVAALTVQA


  Protein sequence of regulator: 925 a.a.    .

>REG0377 NC_002516:c656527-653753 [Pseudomonas aeruginosa PAO1]
MAVKSLVFRRKFPLLVTGSLLALQPVAALTVQAADQFDCKVSATGGWDCSPLQNANANLPPRPAHTATSVSTAAAGSSVS
GSGGETVEAEPTQRLVTESGGRALKSRSADYSHLDWIPREKLTAAQLAEIGPYCGGSYIEPVRPGMDDGAPSDESPTYVS
AKASRYEQEKQIATLAGDVVLRQGSMQVEGDEANLHQLENRGELVGNVKLRDKGMLVVGDHAQVQLDNGEAQVDNAEYVI
HKAHARGSALYAKRSENAIIMLKDGTYTRCEPSSNAWTLKGNNVKLNPATGFGTATNATLRVKDFPVFYTPYIYFPIDDR
RQSGFLPPSFSSTSDTGFTLVTPYYFNLAPNYDATLYPRYMAKRGMMLEGEFRYLTHSSEGIVNAAYLNDKDDHREGFPD
YSKDRWLYGLKNTTGLDSRWLAEVDYTRISDPYYFQDLDTDLGVGSTTYVNQRGTLTYRGDTFTGRLNAQAYQLATTTDV
TPYDRLPQITFDGFLPYNPGGMQFTYGTEFVRFDRDLDENIYFNDDGSIRGKRPDASLQGLARATGDRMHLEPGMSLPMT
RSWGYVTPTLKYLYTKYDLDLDSQGKTDLNKRDESFDSNQDRSLPLVKVDSGLYFDRDTTFAGTPFRQTLEPRAMYLYVP
YKDQDSLPVFDTSEPSFSYDSLWRENRFTGKDRIGDANQLSLGVTSRFIEENGFERASISAGQIYYFRDRRVQLPGLTEK
DLKRLNLDPSGLDNDSWRSPYAFAGQYRFNRDWRINSDFNWNPNTSRTESGSAIFHYQPEVDPGKVVNVGYRYRADARRF
DSSRGTFRYGNENDIIKQHDFSVIWPLVPQWSVLARWQYDYNKNRTLEAFGGFEYDSCCWKLRLINRYWLDVDDDAFLVQ
SEKADRGIFLQIVLKGLGGIVGNKTEMFLDKGIQGYRQREDQAM*

  Nucleotide sequence of regulator: 2775 bp    .

>REG0377 NC_002516:c656527-653753 [Pseudomonas aeruginosa PAO1]
ATGGCAGTGAAATCCCTCGTGTTTCGAAGAAAGTTTCCCCTGCTGGTCACCGGCAGCCTGCTGGCTCTGCAGCCGGTCGC
AGCATTGACTGTACAGGCCGCCGATCAGTTCGACTGCAAGGTATCGGCCACCGGCGGCTGGGATTGCTCCCCGCTGCAGA
ACGCCAATGCCAACCTGCCACCGCGCCCGGCGCATACCGCGACCTCGGTCAGCACCGCCGCGGCGGGCAGCAGCGTGTCC
GGCAGCGGAGGCGAAACCGTCGAGGCAGAGCCGACCCAGCGCCTGGTCACCGAGTCCGGCGGTCGCGCGCTGAAATCCCG
CAGTGCGGACTATTCGCACCTCGACTGGATTCCTCGGGAAAAACTGACCGCCGCCCAGTTGGCGGAAATCGGCCCCTATT
GCGGCGGCTCCTACATCGAGCCGGTGCGTCCGGGCATGGATGACGGCGCGCCCAGCGACGAGAGCCCGACCTACGTATCC
GCCAAGGCTTCGCGCTACGAGCAGGAAAAACAGATCGCCACCCTCGCCGGTGACGTGGTCCTGCGCCAGGGAAGCATGCA
GGTGGAAGGCGACGAGGCCAACCTGCACCAGCTGGAAAACCGCGGCGAACTGGTCGGCAACGTCAAGCTGCGCGACAAGG
GCATGCTGGTGGTCGGCGACCACGCCCAGGTCCAGCTGGACAACGGCGAGGCCCAGGTCGACAACGCCGAGTACGTGATC
CACAAGGCCCATGCCCGCGGCAGCGCGCTTTACGCCAAGCGCAGCGAGAACGCCATCATCATGCTCAAGGATGGCACCTA
CACCCGCTGCGAACCGAGCAGCAACGCCTGGACCCTGAAGGGCAACAACGTCAAGCTGAACCCGGCCACCGGCTTCGGCA
CCGCGACCAACGCGACCCTGCGGGTCAAAGATTTCCCGGTGTTCTACACCCCGTACATCTATTTCCCGATCGACGACCGC
CGCCAGTCCGGTTTCCTGCCGCCGAGCTTCAGCAGCACCAGCGACACCGGCTTCACCCTGGTCACCCCGTACTACTTCAA
CCTGGCGCCGAACTACGACGCCACGTTGTACCCGCGCTACATGGCCAAGCGCGGCATGATGCTGGAAGGCGAGTTCCGCT
ACCTGACCCACAGCAGCGAAGGTATCGTCAACGCTGCCTACCTTAACGACAAGGACGATCACCGCGAAGGTTTCCCGGAC
TACAGCAAGGACCGCTGGCTGTACGGTCTCAAGAACACCACCGGCCTCGATTCGCGCTGGCTGGCCGAGGTCGACTACAC
GCGGATCAGTGATCCCTATTACTTCCAGGATCTGGATACTGACCTTGGCGTAGGCAGCACTACGTACGTCAACCAACGGG
GCACTCTGACCTATCGCGGGGACACCTTTACTGGCCGTTTGAATGCGCAGGCGTACCAGTTGGCGACCACCACCGATGTA
ACCCCGTATGACCGGCTGCCACAGATTACCTTCGACGGTTTCCTGCCCTACAACCCAGGTGGAATGCAATTCACCTATGG
CACCGAGTTCGTTCGATTCGATCGAGATCTAGACGAAAATATCTATTTCAATGACGACGGCAGCATCCGCGGCAAACGCC
CAGATGCGTCACTTCAAGGCCTTGCCCGAGCAACGGGAGACCGTATGCATCTGGAGCCGGGCATGAGTTTGCCAATGACG
CGCAGCTGGGGTTATGTGACACCCACATTGAAATATTTGTATACAAAGTACGATCTAGACTTGGATAGCCAGGGCAAGAC
CGACCTGAATAAAAGAGATGAGTCCTTTGACAGCAATCAGGACCGTTCGCTCCCTTTGGTTAAGGTCGACAGCGGCCTGT
ATTTCGACCGAGACACCACCTTTGCCGGCACACCATTCCGCCAAACTCTCGAGCCGCGTGCCATGTACTTGTACGTTCCA
TATAAGGACCAAGACAGCCTGCCGGTCTTCGATACCAGTGAACCGTCCTTCAGTTACGACTCTCTGTGGCGCGAGAACCG
CTTTACCGGTAAGGATCGTATCGGCGATGCCAACCAGTTGTCCCTGGGCGTCACCAGTCGTTTCATCGAAGAGAACGGTT
TCGAACGCGCCAGCATCAGTGCCGGCCAGATTTACTACTTCCGTGATCGTCGCGTCCAACTACCAGGCCTAACCGAGAAG
GATCTGAAGCGACTCAACCTAGATCCCAGCGGCTTGGACAATGACAGCTGGCGCTCTCCCTACGCCTTTGCCGGCCAGTA
CCGTTTCAACCGCGACTGGCGCATCAACTCCGACTTCAATTGGAACCCCAACACCAGTCGTACTGAGTCCGGTAGCGCCA
TCTTCCACTACCAGCCCGAAGTCGATCCGGGCAAGGTGGTCAACGTTGGCTATCGCTATCGTGCAGATGCTCGGCGCTTT
GATTCCTCCCGCGGCACATTCCGATATGGCAACGAAAACGACATCATCAAGCAGCACGATTTCTCGGTCATCTGGCCGCT
GGTGCCGCAGTGGTCCGTGCTCGCCCGCTGGCAGTACGACTACAACAAGAACCGCACCCTCGAGGCCTTCGGCGGCTTCG
AATACGACAGCTGCTGCTGGAAGCTGCGCCTGATCAACCGCTACTGGCTGGATGTGGACGATGACGCCTTCCTCGTGCAG
AGCGAGAAGGCCGACCGCGGCATCTTCCTGCAGATCGTCCTGAAGGGCCTCGGCGGCATCGTAGGCAACAAGACGGAAAT
GTTCCTCGACAAAGGCATCCAAGGTTATCGTCAACGTGAAGATCAAGCTATGTAA