Detailed information of regulator
Summary
| Regulator ID | REG0006  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01033 (Type i3) |
| Strain | Serratia sp. FS14 |
| Replicon | chromosome |
| Sequence | Protein sequence (482 a.a.); Nucleotide sequence (1446 bp) |
| Description | The periplasmic domain and transmembrane domain of PpkA are disposable for secretion of Hcp by the T6SS in Serratia. |
| Reference |
External database links
| Locus tag (Gene) | L085_RS13655 (ppka) |
| Coordinates (Strand) | 3028273..3029718 (-) |
| NCBI ID | 763151328 |
| RefSeq | NZ_CP005927 |
| Uniprot ID | UPI0004993319 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Pkinase | PF00069.27 | 5e-26 | 23..286 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-341 | MSGTGITKPNSNSLPSGYRFNEFEIQEAIGEGGFGIVYRAYDHQLERTIAIKEYMPTSLAKRNDDLSIGLRGERFGKTFQAGLNSFIQEARLLARFSHPGLLHVLRFWEENGTAYMGTQFYSGTTLKNLQAQQPEKIDEAWIRRLLPPLFSAINTIHQEGYLHRDISLDNIQIQESQLPVLLDFGSARKEIGNLSDETEIMLKPGFAPIEQYTENSDGEQGPWTDIYALGAVLHTLIVGSPPPVSVVRSIEDSYQPLTERRPAGYSPELLRTVDRALALKPEDRPQTIDEMAELLHLPIADENEIISTPAAAPENLLVAANPAAAAAAAGAVTARGTQFSR |
| Transmembrane helix | 342-364 | PMMAGAGVAALLVIGAVAWLSGG |
| Inside | 365-481 | KDDAQAVAQNTVASPAQKTLTEQPSAPQTAQPAATEPEKAAPPAQPVALVYFKLQPGEQVSLDGKPQQVTAGENGFASLNLAPGRYQLEIRHNDRLRRQQLSIDTAGTWLVNPATAG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9909 | - | - |
Protein sequence of regulator: 482 a.a. .
>REG0006 NZ_CP005927:c3029718-3028273 [Serratia sp. FS14]
MSGTGITKPNSNSLPSGYRFNEFEIQEAIGEGGFGIVYRAYDHQLERTIAIKEYMPTSLAKRNDDLSIGLRGERFGKTFQ
AGLNSFIQEARLLARFSHPGLLHVLRFWEENGTAYMGTQFYSGTTLKNLQAQQPEKIDEAWIRRLLPPLFSAINTIHQEG
YLHRDISLDNIQIQESQLPVLLDFGSARKEIGNLSDETEIMLKPGFAPIEQYTENSDGEQGPWTDIYALGAVLHTLIVGS
PPPVSVVRSIEDSYQPLTERRPAGYSPELLRTVDRALALKPEDRPQTIDEMAELLHLPIADENEIISTPAAAPENLLVAA
NPAAAAAAAGAVTARGTQFSRPMMAGAGVAALLVIGAVAWLSGGKDDAQAVAQNTVASPAQKTLTEQPSAPQTAQPAATE
PEKAAPPAQPVALVYFKLQPGEQVSLDGKPQQVTAGENGFASLNLAPGRYQLEIRHNDRLRRQQLSIDTAGTWLVNPATA
G*
MSGTGITKPNSNSLPSGYRFNEFEIQEAIGEGGFGIVYRAYDHQLERTIAIKEYMPTSLAKRNDDLSIGLRGERFGKTFQ
AGLNSFIQEARLLARFSHPGLLHVLRFWEENGTAYMGTQFYSGTTLKNLQAQQPEKIDEAWIRRLLPPLFSAINTIHQEG
YLHRDISLDNIQIQESQLPVLLDFGSARKEIGNLSDETEIMLKPGFAPIEQYTENSDGEQGPWTDIYALGAVLHTLIVGS
PPPVSVVRSIEDSYQPLTERRPAGYSPELLRTVDRALALKPEDRPQTIDEMAELLHLPIADENEIISTPAAAPENLLVAA
NPAAAAAAAGAVTARGTQFSRPMMAGAGVAALLVIGAVAWLSGGKDDAQAVAQNTVASPAQKTLTEQPSAPQTAQPAATE
PEKAAPPAQPVALVYFKLQPGEQVSLDGKPQQVTAGENGFASLNLAPGRYQLEIRHNDRLRRQQLSIDTAGTWLVNPATA
G*
Nucleotide sequence of regulator: 1446 bp .
>REG0006 NZ_CP005927:c3029718-3028273 [Serratia sp. FS14]
ATGTCGGGAACCGGGATAACCAAACCGAATTCGAACAGCCTGCCGAGCGGCTATCGATTCAACGAATTCGAGATCCAGGA
AGCGATCGGCGAAGGCGGCTTCGGCATCGTCTACCGCGCCTACGATCACCAACTGGAACGCACCATCGCCATCAAGGAGT
ACATGCCGACCTCGCTGGCGAAACGCAACGACGATCTCAGCATCGGGCTGCGCGGCGAGCGCTTCGGCAAAACTTTCCAG
GCCGGGCTGAACAGCTTTATTCAGGAGGCGCGTCTGCTGGCGCGCTTCTCGCATCCCGGCCTGCTGCACGTGTTGCGCTT
CTGGGAAGAGAACGGCACCGCCTATATGGGCACCCAGTTTTACAGCGGCACCACGCTGAAAAACCTGCAGGCGCAGCAGC
CGGAGAAAATCGACGAGGCGTGGATCCGTCGGCTGCTGCCGCCGCTGTTCAGCGCCATCAACACCATCCATCAGGAAGGT
TATCTGCACCGCGACATTTCGCTGGATAACATCCAGATCCAGGAGAGCCAGCTGCCGGTGCTGCTGGACTTCGGGTCAGC
GCGCAAAGAGATCGGCAACCTGTCGGACGAAACGGAGATCATGCTCAAACCGGGCTTCGCGCCGATCGAGCAGTACACCG
AGAACAGCGACGGCGAACAGGGGCCGTGGACCGATATCTACGCGCTGGGCGCCGTGCTGCACACGCTGATCGTCGGTTCG
CCGCCGCCGGTCAGCGTGGTGCGCAGCATCGAAGACAGCTACCAGCCGCTGACCGAGCGCCGCCCCGCCGGCTACTCGCC
GGAGCTGTTGCGCACCGTCGATCGCGCGCTGGCGCTGAAGCCGGAAGACCGCCCGCAGACCATCGATGAAATGGCCGAGC
TGTTGCATTTGCCGATCGCCGATGAAAACGAAATCATCAGCACCCCGGCCGCCGCGCCGGAGAACTTGTTGGTCGCCGCC
AACCCGGCCGCTGCTGCGGCCGCCGCCGGCGCCGTTACCGCGCGCGGCACCCAATTCTCACGCCCGATGATGGCCGGCGC
CGGCGTCGCGGCGCTGCTGGTGATCGGCGCCGTCGCCTGGCTGAGCGGCGGCAAGGACGATGCCCAGGCCGTCGCCCAAA
ACACCGTAGCGTCACCGGCGCAGAAAACCCTGACGGAGCAGCCGAGCGCGCCGCAAACCGCGCAGCCTGCAGCGACAGAA
CCTGAAAAAGCGGCGCCGCCGGCGCAGCCGGTGGCGCTGGTGTACTTCAAACTGCAACCGGGCGAACAGGTCAGCCTCGA
CGGCAAGCCGCAGCAGGTCACTGCGGGCGAAAACGGCTTTGCCAGCCTGAACCTGGCGCCGGGCCGCTATCAGCTGGAGA
TCCGTCACAACGATCGGCTCCGCCGCCAGCAGCTCAGCATCGACACCGCCGGCACCTGGCTGGTCAACCCGGCCACCGCC
GGTTAA
ATGTCGGGAACCGGGATAACCAAACCGAATTCGAACAGCCTGCCGAGCGGCTATCGATTCAACGAATTCGAGATCCAGGA
AGCGATCGGCGAAGGCGGCTTCGGCATCGTCTACCGCGCCTACGATCACCAACTGGAACGCACCATCGCCATCAAGGAGT
ACATGCCGACCTCGCTGGCGAAACGCAACGACGATCTCAGCATCGGGCTGCGCGGCGAGCGCTTCGGCAAAACTTTCCAG
GCCGGGCTGAACAGCTTTATTCAGGAGGCGCGTCTGCTGGCGCGCTTCTCGCATCCCGGCCTGCTGCACGTGTTGCGCTT
CTGGGAAGAGAACGGCACCGCCTATATGGGCACCCAGTTTTACAGCGGCACCACGCTGAAAAACCTGCAGGCGCAGCAGC
CGGAGAAAATCGACGAGGCGTGGATCCGTCGGCTGCTGCCGCCGCTGTTCAGCGCCATCAACACCATCCATCAGGAAGGT
TATCTGCACCGCGACATTTCGCTGGATAACATCCAGATCCAGGAGAGCCAGCTGCCGGTGCTGCTGGACTTCGGGTCAGC
GCGCAAAGAGATCGGCAACCTGTCGGACGAAACGGAGATCATGCTCAAACCGGGCTTCGCGCCGATCGAGCAGTACACCG
AGAACAGCGACGGCGAACAGGGGCCGTGGACCGATATCTACGCGCTGGGCGCCGTGCTGCACACGCTGATCGTCGGTTCG
CCGCCGCCGGTCAGCGTGGTGCGCAGCATCGAAGACAGCTACCAGCCGCTGACCGAGCGCCGCCCCGCCGGCTACTCGCC
GGAGCTGTTGCGCACCGTCGATCGCGCGCTGGCGCTGAAGCCGGAAGACCGCCCGCAGACCATCGATGAAATGGCCGAGC
TGTTGCATTTGCCGATCGCCGATGAAAACGAAATCATCAGCACCCCGGCCGCCGCGCCGGAGAACTTGTTGGTCGCCGCC
AACCCGGCCGCTGCTGCGGCCGCCGCCGGCGCCGTTACCGCGCGCGGCACCCAATTCTCACGCCCGATGATGGCCGGCGC
CGGCGTCGCGGCGCTGCTGGTGATCGGCGCCGTCGCCTGGCTGAGCGGCGGCAAGGACGATGCCCAGGCCGTCGCCCAAA
ACACCGTAGCGTCACCGGCGCAGAAAACCCTGACGGAGCAGCCGAGCGCGCCGCAAACCGCGCAGCCTGCAGCGACAGAA
CCTGAAAAAGCGGCGCCGCCGGCGCAGCCGGTGGCGCTGGTGTACTTCAAACTGCAACCGGGCGAACAGGTCAGCCTCGA
CGGCAAGCCGCAGCAGGTCACTGCGGGCGAAAACGGCTTTGCCAGCCTGAACCTGGCGCCGGGCCGCTATCAGCTGGAGA
TCCGTCACAACGATCGGCTCCGCCGCCAGCAGCTCAGCATCGACACCGCCGGCACCTGGCTGGTCAACCCGGCCACCGCC
GGTTAA