Detailed information of regulator
Summary
| Regulator ID | REG0077  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00016 (Type i5) |
| Strain | Agrobacterium fabrum str. C58 |
| Replicon | chromosome |
| Sequence | Protein sequence (400 a.a.); Nucleotide sequence (1200 bp) |
| Description | Hcp secretion was completely abolished in FhaΔFHA and FhaR30AS46A strains. |
| Reference |
External database links
| Locus tag (Gene) | Atu4335 (Fha/TagH) |
| Coordinates (Strand) | 1467064..1468263 (-) |
| NCBI ID | 499280697 |
| RefSeq | NC_003063 |
| Uniprot ID | A9CGG1_AGRFC, A0A083ZIC2_RHIRD |
| KEGG ID | atu:Atu4335 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FHA | PF00498.28 | 2e-15 | 28..93 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-399 | MKLALKNTRNIGTGKAGQWSFERGRRAIGRSRDCDWQIDDNERRVSKLHCTLSRDGEGFIILDQSANGTLVDGRLLLEGESARLRDGSQINIGGQIFEVAISGNAELDFSDPDASLKMSDETLTISAILADIAPNGRSAGGLLGNTAAADDWPETRRESAGRKAKSISRNVEIGWSEPPSAQGIGTVLPDDWDTEPAASSDHEHTDALNTPVMISRPVSASHDRDFDSVFPDTDEDPIRQGRLPADTATAQEIGDLLTALERESADCLAILDIESDHAGQGPAPSLSERMETLIGQQRRLAASLETLMHGCTQKLEPRLLEAAVDAGNDFRAKIGRRDWQGLVMKTDYWSAYKKQFEENGRPLPVRQFLQRAARGEAASARETDATAADAKGVNNHDET |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9579 | - | - |
Protein sequence of regulator: 400 a.a. .
>REG0077 NC_003063:c1468263-1467064 [Agrobacterium fabrum str. C58]
MKLALKNTRNIGTGKAGQWSFERGRRAIGRSRDCDWQIDDNERRVSKLHCTLSRDGEGFIILDQSANGTLVDGRLLLEGE
SARLRDGSQINIGGQIFEVAISGNAELDFSDPDASLKMSDETLTISAILADIAPNGRSAGGLLGNTAAADDWPETRRESA
GRKAKSISRNVEIGWSEPPSAQGIGTVLPDDWDTEPAASSDHEHTDALNTPVMISRPVSASHDRDFDSVFPDTDEDPIRQ
GRLPADTATAQEIGDLLTALERESADCLAILDIESDHAGQGPAPSLSERMETLIGQQRRLAASLETLMHGCTQKLEPRLL
EAAVDAGNDFRAKIGRRDWQGLVMKTDYWSAYKKQFEENGRPLPVRQFLQRAARGEAASARETDATAADAKGVNNHDET*
MKLALKNTRNIGTGKAGQWSFERGRRAIGRSRDCDWQIDDNERRVSKLHCTLSRDGEGFIILDQSANGTLVDGRLLLEGE
SARLRDGSQINIGGQIFEVAISGNAELDFSDPDASLKMSDETLTISAILADIAPNGRSAGGLLGNTAAADDWPETRRESA
GRKAKSISRNVEIGWSEPPSAQGIGTVLPDDWDTEPAASSDHEHTDALNTPVMISRPVSASHDRDFDSVFPDTDEDPIRQ
GRLPADTATAQEIGDLLTALERESADCLAILDIESDHAGQGPAPSLSERMETLIGQQRRLAASLETLMHGCTQKLEPRLL
EAAVDAGNDFRAKIGRRDWQGLVMKTDYWSAYKKQFEENGRPLPVRQFLQRAARGEAASARETDATAADAKGVNNHDET*
Nucleotide sequence of regulator: 1200 bp .
>REG0077 NC_003063:c1468263-1467064 [Agrobacterium fabrum str. C58]
ATGAAGCTTGCACTCAAGAACACACGGAACATCGGGACGGGAAAAGCCGGCCAGTGGAGCTTCGAGCGAGGCCGCCGCGC
AATTGGCCGGTCTCGTGATTGCGACTGGCAGATCGATGACAATGAGCGGCGCGTTTCCAAGCTGCATTGCACGCTGAGCC
GCGACGGCGAAGGTTTCATCATCCTCGACCAGAGCGCAAACGGCACGCTCGTCGATGGCCGTCTGTTGCTTGAGGGCGAA
AGCGCGCGTCTTCGGGACGGTTCGCAGATCAATATCGGCGGGCAGATTTTCGAGGTCGCCATCAGCGGCAATGCGGAGCT
CGATTTTTCCGATCCCGACGCCTCGCTGAAGATGAGTGACGAGACGCTGACCATTTCGGCGATCCTTGCCGATATCGCCC
CGAACGGCCGTTCTGCCGGCGGCCTGCTGGGCAACACCGCCGCCGCCGATGACTGGCCGGAAACACGGCGCGAAAGCGCG
GGCCGGAAGGCGAAGTCCATTTCGCGCAACGTCGAGATCGGCTGGAGCGAACCGCCCTCTGCGCAGGGAATTGGCACTGT
TCTGCCTGATGACTGGGATACGGAGCCGGCTGCAAGCAGCGACCACGAACATACCGATGCGCTGAACACGCCGGTCATGA
TCTCACGTCCGGTCTCGGCTTCGCATGACCGGGATTTTGACAGCGTTTTCCCGGATACAGACGAAGACCCGATCCGCCAG
GGCAGATTGCCCGCGGACACGGCGACCGCACAGGAGATCGGCGATCTTCTGACGGCACTGGAAAGGGAAAGTGCCGATTG
CCTGGCCATCCTCGACATTGAGAGCGATCATGCCGGCCAAGGCCCGGCACCATCCCTCAGCGAGCGAATGGAAACGCTCA
TTGGCCAGCAGCGCCGTCTTGCCGCCTCGCTCGAAACCCTCATGCATGGCTGCACGCAGAAACTCGAGCCCCGGCTTCTT
GAGGCCGCTGTCGATGCCGGCAACGATTTTCGCGCGAAGATCGGGCGCAGAGACTGGCAGGGGCTGGTCATGAAGACCGA
CTACTGGTCGGCTTACAAAAAACAATTCGAAGAAAACGGCCGTCCATTGCCGGTCAGGCAGTTCCTGCAGCGGGCAGCGC
GTGGTGAAGCCGCGTCCGCACGGGAGACTGACGCGACCGCGGCCGATGCCAAGGGGGTAAACAACCACGATGAGACATGA
ATGAAGCTTGCACTCAAGAACACACGGAACATCGGGACGGGAAAAGCCGGCCAGTGGAGCTTCGAGCGAGGCCGCCGCGC
AATTGGCCGGTCTCGTGATTGCGACTGGCAGATCGATGACAATGAGCGGCGCGTTTCCAAGCTGCATTGCACGCTGAGCC
GCGACGGCGAAGGTTTCATCATCCTCGACCAGAGCGCAAACGGCACGCTCGTCGATGGCCGTCTGTTGCTTGAGGGCGAA
AGCGCGCGTCTTCGGGACGGTTCGCAGATCAATATCGGCGGGCAGATTTTCGAGGTCGCCATCAGCGGCAATGCGGAGCT
CGATTTTTCCGATCCCGACGCCTCGCTGAAGATGAGTGACGAGACGCTGACCATTTCGGCGATCCTTGCCGATATCGCCC
CGAACGGCCGTTCTGCCGGCGGCCTGCTGGGCAACACCGCCGCCGCCGATGACTGGCCGGAAACACGGCGCGAAAGCGCG
GGCCGGAAGGCGAAGTCCATTTCGCGCAACGTCGAGATCGGCTGGAGCGAACCGCCCTCTGCGCAGGGAATTGGCACTGT
TCTGCCTGATGACTGGGATACGGAGCCGGCTGCAAGCAGCGACCACGAACATACCGATGCGCTGAACACGCCGGTCATGA
TCTCACGTCCGGTCTCGGCTTCGCATGACCGGGATTTTGACAGCGTTTTCCCGGATACAGACGAAGACCCGATCCGCCAG
GGCAGATTGCCCGCGGACACGGCGACCGCACAGGAGATCGGCGATCTTCTGACGGCACTGGAAAGGGAAAGTGCCGATTG
CCTGGCCATCCTCGACATTGAGAGCGATCATGCCGGCCAAGGCCCGGCACCATCCCTCAGCGAGCGAATGGAAACGCTCA
TTGGCCAGCAGCGCCGTCTTGCCGCCTCGCTCGAAACCCTCATGCATGGCTGCACGCAGAAACTCGAGCCCCGGCTTCTT
GAGGCCGCTGTCGATGCCGGCAACGATTTTCGCGCGAAGATCGGGCGCAGAGACTGGCAGGGGCTGGTCATGAAGACCGA
CTACTGGTCGGCTTACAAAAAACAATTCGAAGAAAACGGCCGTCCATTGCCGGTCAGGCAGTTCCTGCAGCGGGCAGCGC
GTGGTGAAGCCGCGTCCGCACGGGAGACTGACGCGACCGCGGCCGATGCCAAGGGGGTAAACAACCACGATGAGACATGA