Detailed information of regulator


Summary

Regulator ID REG0078 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (302 a.a.); Nucleotide sequence (906 bp)
Description The reduced Hcp secretion in ΔppkA could be fully restored by trans complementation and PpkA can phosphorylate TssL and phosphorylation of TssL at Thr 14 are critical for type VI secretion.
Reference

External database links

Locus tag (Gene) Atu4330 (PpkA)
Coordinates (Strand) 1458366..1459271 (-)
NCBI ID 737274050
RefSeq NC_003063
Uniprot ID UPI0004D6E43A
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
Unknown -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Pkinase PF00069.27 2.1e-20 86..264

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-301 VSKARHPLDEPAYPEVAGRFSDLWRSIRHDGMRERSDQEQKDLIDQIRNALDRKTSGVSGEKPDWIANSFAVNCTAYQSETTLLLQLRHRDLGSLHALKTVPPGSRDDAVLLQRLRDEAKIGLALRHPCLAETSALLRLPDGRPGLLQPWFAHSLASIVSTQPIAASQVISILRRVLEGLSAMHALGYVHCDVTPANILLADGNFETAKLGDFGIALRIGRKHAEQGMRFAGSPEFAAPEQRQGAPAKPDQDIYAVGRLARRLIATDEAQSPQRLADFAQACCHDDPALRPQTAAEALQLL



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9891 - -



  Protein sequence of regulator: 302 a.a.    .

>REG0078 NC_003063:c1459271-1458366 [Agrobacterium fabrum str. C58]
VSKARHPLDEPAYPEVAGRFSDLWRSIRHDGMRERSDQEQKDLIDQIRNALDRKTSGVSGEKPDWIANSFAVNCTAYQSE
TTLLLQLRHRDLGSLHALKTVPPGSRDDAVLLQRLRDEAKIGLALRHPCLAETSALLRLPDGRPGLLQPWFAHSLASIVS
TQPIAASQVISILRRVLEGLSAMHALGYVHCDVTPANILLADGNFETAKLGDFGIALRIGRKHAEQGMRFAGSPEFAAPE
QRQGAPAKPDQDIYAVGRLARRLIATDEAQSPQRLADFAQACCHDDPALRPQTAAEALQLL*

  Nucleotide sequence of regulator: 906 bp    .

>REG0078 NC_003063:c1459271-1458366 [Agrobacterium fabrum str. C58]
GTGAGCAAGGCTAGACATCCACTTGATGAGCCTGCCTATCCGGAGGTAGCCGGACGTTTTTCCGACCTATGGCGAAGCAT
TCGCCATGATGGCATGCGGGAAAGAAGCGATCAGGAGCAAAAAGACCTGATCGACCAGATCCGAAATGCGTTGGATCGAA
AAACATCTGGCGTTTCCGGCGAAAAACCGGACTGGATCGCGAACAGCTTTGCCGTGAATTGCACAGCCTACCAGAGCGAG
ACGACGCTTCTGCTACAATTGCGCCACCGCGATCTCGGCTCGCTGCACGCGCTGAAAACCGTGCCACCGGGCAGTCGCGA
CGACGCTGTTCTGCTGCAACGCTTGCGGGACGAGGCCAAGATCGGGCTCGCGCTGCGCCATCCCTGCCTTGCGGAAACAT
CAGCGCTTCTGAGATTGCCCGATGGCCGGCCTGGCCTTTTGCAGCCATGGTTCGCGCACTCGCTGGCATCGATCGTATCT
ACACAACCGATCGCCGCCTCGCAGGTGATCTCGATCCTTCGCCGCGTGCTGGAAGGGCTGAGTGCCATGCATGCACTTGG
ATATGTGCATTGCGACGTCACGCCAGCCAATATTCTGCTGGCGGATGGTAATTTCGAAACGGCGAAACTGGGTGATTTCG
GCATCGCCCTGCGGATTGGCCGGAAACACGCGGAACAGGGCATGCGCTTTGCCGGCTCGCCTGAATTCGCGGCACCGGAA
CAAAGGCAAGGCGCACCCGCAAAGCCGGATCAGGACATATACGCCGTGGGACGGCTCGCCCGGCGGCTGATCGCGACGGA
TGAGGCGCAGTCACCGCAACGCCTCGCAGATTTTGCGCAGGCCTGTTGCCACGACGATCCTGCCCTACGTCCGCAAACGG
CGGCGGAAGCGCTACAGCTTCTGTAG