Detailed information of regulator
Summary
| Regulator ID | REG0078  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00016 (Type i5) |
| Strain | Agrobacterium fabrum str. C58 |
| Replicon | chromosome |
| Sequence | Protein sequence (302 a.a.); Nucleotide sequence (906 bp) |
| Description | The reduced Hcp secretion in ΔppkA could be fully restored by trans complementation and PpkA can phosphorylate TssL and phosphorylation of TssL at Thr 14 are critical for type VI secretion. |
| Reference |
External database links
| Locus tag (Gene) | Atu4330 (PpkA) |
| Coordinates (Strand) | 1458366..1459271 (-) |
| NCBI ID | 737274050 |
| RefSeq | NC_003063 |
| Uniprot ID | UPI0004D6E43A |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Pkinase | PF00069.27 | 2.1e-20 | 86..264 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-301 | VSKARHPLDEPAYPEVAGRFSDLWRSIRHDGMRERSDQEQKDLIDQIRNALDRKTSGVSGEKPDWIANSFAVNCTAYQSETTLLLQLRHRDLGSLHALKTVPPGSRDDAVLLQRLRDEAKIGLALRHPCLAETSALLRLPDGRPGLLQPWFAHSLASIVSTQPIAASQVISILRRVLEGLSAMHALGYVHCDVTPANILLADGNFETAKLGDFGIALRIGRKHAEQGMRFAGSPEFAAPEQRQGAPAKPDQDIYAVGRLARRLIATDEAQSPQRLADFAQACCHDDPALRPQTAAEALQLL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9891 | - | - |
Protein sequence of regulator: 302 a.a. .
>REG0078 NC_003063:c1459271-1458366 [Agrobacterium fabrum str. C58]
VSKARHPLDEPAYPEVAGRFSDLWRSIRHDGMRERSDQEQKDLIDQIRNALDRKTSGVSGEKPDWIANSFAVNCTAYQSE
TTLLLQLRHRDLGSLHALKTVPPGSRDDAVLLQRLRDEAKIGLALRHPCLAETSALLRLPDGRPGLLQPWFAHSLASIVS
TQPIAASQVISILRRVLEGLSAMHALGYVHCDVTPANILLADGNFETAKLGDFGIALRIGRKHAEQGMRFAGSPEFAAPE
QRQGAPAKPDQDIYAVGRLARRLIATDEAQSPQRLADFAQACCHDDPALRPQTAAEALQLL*
VSKARHPLDEPAYPEVAGRFSDLWRSIRHDGMRERSDQEQKDLIDQIRNALDRKTSGVSGEKPDWIANSFAVNCTAYQSE
TTLLLQLRHRDLGSLHALKTVPPGSRDDAVLLQRLRDEAKIGLALRHPCLAETSALLRLPDGRPGLLQPWFAHSLASIVS
TQPIAASQVISILRRVLEGLSAMHALGYVHCDVTPANILLADGNFETAKLGDFGIALRIGRKHAEQGMRFAGSPEFAAPE
QRQGAPAKPDQDIYAVGRLARRLIATDEAQSPQRLADFAQACCHDDPALRPQTAAEALQLL*
Nucleotide sequence of regulator: 906 bp .
>REG0078 NC_003063:c1459271-1458366 [Agrobacterium fabrum str. C58]
GTGAGCAAGGCTAGACATCCACTTGATGAGCCTGCCTATCCGGAGGTAGCCGGACGTTTTTCCGACCTATGGCGAAGCAT
TCGCCATGATGGCATGCGGGAAAGAAGCGATCAGGAGCAAAAAGACCTGATCGACCAGATCCGAAATGCGTTGGATCGAA
AAACATCTGGCGTTTCCGGCGAAAAACCGGACTGGATCGCGAACAGCTTTGCCGTGAATTGCACAGCCTACCAGAGCGAG
ACGACGCTTCTGCTACAATTGCGCCACCGCGATCTCGGCTCGCTGCACGCGCTGAAAACCGTGCCACCGGGCAGTCGCGA
CGACGCTGTTCTGCTGCAACGCTTGCGGGACGAGGCCAAGATCGGGCTCGCGCTGCGCCATCCCTGCCTTGCGGAAACAT
CAGCGCTTCTGAGATTGCCCGATGGCCGGCCTGGCCTTTTGCAGCCATGGTTCGCGCACTCGCTGGCATCGATCGTATCT
ACACAACCGATCGCCGCCTCGCAGGTGATCTCGATCCTTCGCCGCGTGCTGGAAGGGCTGAGTGCCATGCATGCACTTGG
ATATGTGCATTGCGACGTCACGCCAGCCAATATTCTGCTGGCGGATGGTAATTTCGAAACGGCGAAACTGGGTGATTTCG
GCATCGCCCTGCGGATTGGCCGGAAACACGCGGAACAGGGCATGCGCTTTGCCGGCTCGCCTGAATTCGCGGCACCGGAA
CAAAGGCAAGGCGCACCCGCAAAGCCGGATCAGGACATATACGCCGTGGGACGGCTCGCCCGGCGGCTGATCGCGACGGA
TGAGGCGCAGTCACCGCAACGCCTCGCAGATTTTGCGCAGGCCTGTTGCCACGACGATCCTGCCCTACGTCCGCAAACGG
CGGCGGAAGCGCTACAGCTTCTGTAG
GTGAGCAAGGCTAGACATCCACTTGATGAGCCTGCCTATCCGGAGGTAGCCGGACGTTTTTCCGACCTATGGCGAAGCAT
TCGCCATGATGGCATGCGGGAAAGAAGCGATCAGGAGCAAAAAGACCTGATCGACCAGATCCGAAATGCGTTGGATCGAA
AAACATCTGGCGTTTCCGGCGAAAAACCGGACTGGATCGCGAACAGCTTTGCCGTGAATTGCACAGCCTACCAGAGCGAG
ACGACGCTTCTGCTACAATTGCGCCACCGCGATCTCGGCTCGCTGCACGCGCTGAAAACCGTGCCACCGGGCAGTCGCGA
CGACGCTGTTCTGCTGCAACGCTTGCGGGACGAGGCCAAGATCGGGCTCGCGCTGCGCCATCCCTGCCTTGCGGAAACAT
CAGCGCTTCTGAGATTGCCCGATGGCCGGCCTGGCCTTTTGCAGCCATGGTTCGCGCACTCGCTGGCATCGATCGTATCT
ACACAACCGATCGCCGCCTCGCAGGTGATCTCGATCCTTCGCCGCGTGCTGGAAGGGCTGAGTGCCATGCATGCACTTGG
ATATGTGCATTGCGACGTCACGCCAGCCAATATTCTGCTGGCGGATGGTAATTTCGAAACGGCGAAACTGGGTGATTTCG
GCATCGCCCTGCGGATTGGCCGGAAACACGCGGAACAGGGCATGCGCTTTGCCGGCTCGCCTGAATTCGCGGCACCGGAA
CAAAGGCAAGGCGCACCCGCAAAGCCGGATCAGGACATATACGCCGTGGGACGGCTCGCCCGGCGGCTGATCGCGACGGA
TGAGGCGCAGTCACCGCAACGCCTCGCAGATTTTGCGCAGGCCTGTTGCCACGACGATCCTGCCCTACGTCCGCAAACGG
CGGCGGAAGCGCTACAGCTTCTGTAG