Detailed information of accessory protein


Summary

Accessory protein ID ACP00011 experimental
Type adaptor
Related T6SS (Type) T6SS00016 (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Related structure protein Atu3642 (VgrG2)
Related effector Atu3640 (Tde2)
Description Atu3641, a DUF2169-containing protein, is required for Tde2-dependent antibacterial activity
Reference [1] Bondage DD, Lin JS, Ma LS, et al. VgrG C terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3931-40. doi: 10.1073/pnas.1600428113. Epub 2016 Jun 16. PMID: 27313214
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Atu3641
Coordinates (Strand) 693818..694927 (-)
NCBI ID 499279473
RefSeq NC_003063
Uniprot ID A9CFH3_AGRFC
KEGG ID atu:Atu3641
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF2169 PF09937.11 2.9e-90 23..336
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-369 MELQNRLPFPAMAFRQFDAEGGRDCIVSVRATFTHVQDGTMEIAREQESFQWEDAYEGDPHQTVLLRQSDLTPDKPGTDISFLGDAWSPSQEPQKSWRASIRVGDVSKEIDVCGQRFWKPVIKEKWAGFYAREPKRVISDWVLNDPEPARQVAVCWTNAFGGTIPGTGDTETDTPADVERRNLLGCGIVNLDMPADTPPVRAPQITSPGERLDWKDAPEPQGFGLVSPWWRSRQQYTGTYDDAWVAERHPLLPRDFDPRFWQAAHPDLVATPHLKGDEKYELVNLSSEHPVAKGSLPGLTLGVHCSREDRDEWHVLKLDGVQFDWRDDGRVLLTWRVSFPLPDAGETTLTLTRVRLKAPETEAAARETA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9872 - -


  Protein sequence: 370 a.a.    

>ACP00011 NC_003063:c694927-693818 [Agrobacterium fabrum str. C58]
MELQNRLPFPAMAFRQFDAEGGRDCIVSVRATFTHVQDGTMEIAREQESFQWEDAYEGDPHQTVLLRQSDLTPDKPGTDI
SFLGDAWSPSQEPQKSWRASIRVGDVSKEIDVCGQRFWKPVIKEKWAGFYAREPKRVISDWVLNDPEPARQVAVCWTNAF
GGTIPGTGDTETDTPADVERRNLLGCGIVNLDMPADTPPVRAPQITSPGERLDWKDAPEPQGFGLVSPWWRSRQQYTGTY
DDAWVAERHPLLPRDFDPRFWQAAHPDLVATPHLKGDEKYELVNLSSEHPVAKGSLPGLTLGVHCSREDRDEWHVLKLDG
VQFDWRDDGRVLLTWRVSFPLPDAGETTLTLTRVRLKAPETEAAARETA*

  Nucleotide sequence: 1110 bp    

>ACP00011 NC_003063:c694927-693818 [Agrobacterium fabrum str. C58]
TTGGAACTTCAGAACCGCCTGCCGTTTCCCGCGATGGCTTTCCGTCAGTTCGATGCCGAAGGAGGGCGCGATTGTATCGT
CTCCGTGCGCGCTACCTTCACGCATGTGCAGGACGGAACAATGGAGATCGCTCGTGAGCAGGAGAGCTTTCAATGGGAAG
ACGCTTACGAGGGTGATCCGCATCAAACAGTCCTCCTGCGGCAGAGCGATCTGACCCCTGACAAGCCCGGCACGGATATC
AGCTTCCTGGGAGATGCATGGTCACCGTCGCAGGAGCCACAGAAATCCTGGCGCGCCTCCATTCGTGTCGGTGATGTTTC
CAAGGAGATCGATGTATGCGGTCAACGATTCTGGAAACCCGTCATCAAGGAAAAGTGGGCAGGGTTCTACGCAAGGGAGC
CGAAGCGGGTCATCAGCGATTGGGTGCTGAACGATCCCGAGCCGGCCCGTCAGGTCGCTGTCTGCTGGACGAATGCCTTT
GGCGGCACCATTCCAGGCACAGGTGATACCGAAACCGACACCCCTGCGGATGTGGAGCGTCGCAATCTTCTGGGGTGCGG
TATCGTTAATCTCGACATGCCCGCAGACACGCCTCCCGTACGCGCGCCTCAGATCACGTCGCCCGGCGAACGACTGGACT
GGAAGGACGCGCCGGAGCCACAGGGATTTGGCCTGGTATCGCCTTGGTGGCGTTCCCGTCAGCAATATACCGGAACCTAT
GACGATGCATGGGTTGCGGAACGGCATCCGCTTTTGCCTCGTGATTTCGATCCACGCTTCTGGCAGGCCGCCCATCCTGA
TCTCGTCGCGACGCCTCACCTCAAGGGTGACGAGAAATACGAGCTCGTTAACCTGAGTTCTGAGCATCCGGTCGCGAAAG
GCTCGCTGCCCGGCCTCACCCTCGGTGTCCATTGCTCTCGCGAGGATCGCGACGAATGGCATGTGCTGAAACTAGATGGC
GTGCAATTCGACTGGCGCGATGACGGACGAGTGCTTCTGACCTGGCGCGTCAGCTTTCCGCTTCCCGATGCTGGTGAAAC
AACGCTTACCCTGACCAGGGTTCGCTTGAAGGCTCCTGAAACCGAAGCGGCTGCAAGGGAGACAGCATGA