Detailed information of component protein


Summary

Component ID T6CP011404
Component protein type FHA
T6SS ID (Type) T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome linear
Sequence Protein sequence (400 a.a.); Nucleotide sequence (1200 bp)
Reference
[1] Ma LS, Narberhaus F, Lai EM. IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion. J Biol Chem. 2012 May 4;287(19):15610-21. doi: 10.1074/jbc.M111.301630. Epub 2012 Mar 5. PMID: 22393043
[2] Wu HY, Chung PC, Shih HW, et al. Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J Bacteriol. 2008 Apr;190(8):2841-50. doi: 10.1128/JB.01775-07. Epub 2008 Feb 8. PMID: 18263727
[3] Wu CF, Lin JS, Shaw GC, et al. Acid-induced type VI secretion system is regulated by ExoR-ChvG/ChvI signaling cascade in Agrobacterium tumefaciens. PLoS Pathog. 2012 Sep;8(9):e1002938. doi: 10.1371/journal.ppat.1002938. Epub 2012 Sep 27. PMID: 23028331
[4] Lin JS, Ma LS, Lai EM. Systematic dissection of the agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One. 2013 Jul 5;8(7):e67647. doi: 10.1371/journal.pone.0067647. Print 2013. PMID: 23861778
[5] Ma LS, Hachani A, Lin JS, et al. Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe. 2014 Jul 9;16(1):94-104. doi: 10.1016/j.chom.2014.06.002. Epub 2014 Jun 26. PMID: 24981331
[6] Heckel BC, Tomlinson AD, Morton ER, et al. Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression. J Bacteriol. 2014 Sep;196(18):3221-33. doi: 10.1128/JB.01751-14. Epub 2014 Jun 30. PMID: 24982308
[7] Lin JS, Wu HH, Hsu PH, et al. Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathog. 2014 Mar 13;10(3):e1003991. doi: 10.1371/journal.ppat.1003991. eCollection 2014 Mar. PMID: 24626341
[8] Heckel BC, Tomlinson AD, Morton ER, et al. Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression. J Bacteriol. 2014 Sep;196(18):3221-33. doi: 10.1128/JB.01751-14. Epub 2014 Jun 30. PMID: 24982308
[9] Ma LS, Lin JS, Lai EM. An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J Bacteriol. 2009 Jul;191(13):4316-29. doi: 10.1128/JB.00029-09. Epub 2009 Apr 24. PMID: 19395482
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ATU_RS20300
Coordinate (Strand) 1467064..1468263 (-)
NCBI ID WP_010973758.1
RefSeq NC_003063
Uniprot ID A0A7Y0T2X4_9HYPH, A9CGG1_AGRFC, A0A083ZIC2_RHIRD
KEGG ID atu:Atu4335
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
FHA PF00498.28 2e-15 28..93
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-399 MKLALKNTRNIGTGKAGQWSFERGRRAIGRSRDCDWQIDDNERRVSKLHCTLSRDGEGFIILDQSANGTLVDGRLLLEGESARLRDGSQINIGGQIFEVAISGNAELDFSDPDASLKMSDETLTISAILADIAPNGRSAGGLLGNTAAADDWPETRRESAGRKAKSISRNVEIGWSEPPSAQGIGTVLPDDWDTEPAASSDHEHTDALNTPVMISRPVSASHDRDFDSVFPDTDEDPIRQGRLPADTATAQEIGDLLTALERESADCLAILDIESDHAGQGPAPSLSERMETLIGQQRRLAASLETLMHGCTQKLEPRLLEAAVDAGNDFRAKIGRRDWQGLVMKTDYWSAYKKQFEENGRPLPVRQFLQRAARGEAASARETDATAADAKGVNNHDET


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9579 - -


  Protein sequence: 400 a.a.    

>T6CP011404 NC_003063:c1468263-1467064 [Agrobacterium fabrum str. C58] [FHA]
MKLALKNTRNIGTGKAGQWSFERGRRAIGRSRDCDWQIDDNERRVSKLHCTLSRDGEGFIILDQSANGTLVDGRLLLEGE
SARLRDGSQINIGGQIFEVAISGNAELDFSDPDASLKMSDETLTISAILADIAPNGRSAGGLLGNTAAADDWPETRRESA
GRKAKSISRNVEIGWSEPPSAQGIGTVLPDDWDTEPAASSDHEHTDALNTPVMISRPVSASHDRDFDSVFPDTDEDPIRQ
GRLPADTATAQEIGDLLTALERESADCLAILDIESDHAGQGPAPSLSERMETLIGQQRRLAASLETLMHGCTQKLEPRLL
EAAVDAGNDFRAKIGRRDWQGLVMKTDYWSAYKKQFEENGRPLPVRQFLQRAARGEAASARETDATAADAKGVNNHDET*

  Nucleotide sequence: 1200 bp    

>T6CP011404 NC_003063:c1468263-1467064 [Agrobacterium fabrum str. C58] [FHA]
ATGAAGCTTGCACTCAAGAACACACGGAACATCGGGACGGGAAAAGCCGGCCAGTGGAGCTTCGAGCGAGGCCGCCGCGC
AATTGGCCGGTCTCGTGATTGCGACTGGCAGATCGATGACAATGAGCGGCGCGTTTCCAAGCTGCATTGCACGCTGAGCC
GCGACGGCGAAGGTTTCATCATCCTCGACCAGAGCGCAAACGGCACGCTCGTCGATGGCCGTCTGTTGCTTGAGGGCGAA
AGCGCGCGTCTTCGGGACGGTTCGCAGATCAATATCGGCGGGCAGATTTTCGAGGTCGCCATCAGCGGCAATGCGGAGCT
CGATTTTTCCGATCCCGACGCCTCGCTGAAGATGAGTGACGAGACGCTGACCATTTCGGCGATCCTTGCCGATATCGCCC
CGAACGGCCGTTCTGCCGGCGGCCTGCTGGGCAACACCGCCGCCGCCGATGACTGGCCGGAAACACGGCGCGAAAGCGCG
GGCCGGAAGGCGAAGTCCATTTCGCGCAACGTCGAGATCGGCTGGAGCGAACCGCCCTCTGCGCAGGGAATTGGCACTGT
TCTGCCTGATGACTGGGATACGGAGCCGGCTGCAAGCAGCGACCACGAACATACCGATGCGCTGAACACGCCGGTCATGA
TCTCACGTCCGGTCTCGGCTTCGCATGACCGGGATTTTGACAGCGTTTTCCCGGATACAGACGAAGACCCGATCCGCCAG
GGCAGATTGCCCGCGGACACGGCGACCGCACAGGAGATCGGCGATCTTCTGACGGCACTGGAAAGGGAAAGTGCCGATTG
CCTGGCCATCCTCGACATTGAGAGCGATCATGCCGGCCAAGGCCCGGCACCATCCCTCAGCGAGCGAATGGAAACGCTCA
TTGGCCAGCAGCGCCGTCTTGCCGCCTCGCTCGAAACCCTCATGCATGGCTGCACGCAGAAACTCGAGCCCCGGCTTCTT
GAGGCCGCTGTCGATGCCGGCAACGATTTTCGCGCGAAGATCGGGCGCAGAGACTGGCAGGGGCTGGTCATGAAGACCGA
CTACTGGTCGGCTTACAAAAAACAATTCGAAGAAAACGGCCGTCCATTGCCGGTCAGGCAGTTCCTGCAGCGGGCAGCGC
GTGGTGAAGCCGCGTCCGCACGGGAGACTGACGCGACCGCGGCCGATGCCAAGGGGGTAAACAACCACGATGAGACATGA