Detailed information of regulator


Summary

Regulator ID REG0080 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (270 a.a.); Nucleotide sequence (810 bp)
Description High levels of c-di-GMP are capable of down-regulating the T6SS, as seen by monitoring Hcp, ClpV and TssB production.
Reference
External database links

Locus tag (Gene) Atu2091
Coordinates (Strand) 2054055..2054864 (-)
NCBI ID 159185045
RefSeq NC_003063
Uniprot ID Q7CXW5_AGRFC
KEGG ID atu:Atu2091
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
the levels of c-di-GMP ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
ADH_zinc_N PF00107.28 8.9e-14 212..270
ADH_N PF08240.14 3.3e-06 90..148
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-269 ILSIELLEAQYLINMRMDVYASSTSSFVDSLQGMDRASKQVSCERGRDLPPHRPSDN*KGKEGMKAAVYDQAGPPDVLTYRDVADPIVGPDDVLIAVEAISIEGGDLINRRSTPPPGRPWIVGYAASGRVVGAGANVRDRKVGDRVTAFDMQGSHAELWAVPAIRTWLLPSGVDAASAAALPISFGTAHHCLFARGGLLRNQTVLVQAAAGGVGLAAVQLAAQAGATVIAVSSGESRLQRISSLGADHVVDRSMGNVVEAVRQNTGGKG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9637 - -


  Protein sequence of regulator: 270 a.a.    .

>REG0080 NC_003063:c2054864-2054055 [Agrobacterium fabrum str. C58]
ILSIELLEAQYLINMRMDVYASSTSSFVDSLQGMDRASKQVSCERGRDLPPHRPSDN*KGKEGMKAAVYDQAGPPDVLTY
RDVADPIVGPDDVLIAVEAISIEGGDLINRRSTPPPGRPWIVGYAASGRVVGAGANVRDRKVGDRVTAFDMQGSHAELWA
VPAIRTWLLPSGVDAASAAALPISFGTAHHCLFARGGLLRNQTVLVQAAAGGVGLAAVQLAAQAGATVIAVSSGESRLQR
ISSLGADHVVDRSMGNVVEAVRQNTGGKGV

  Nucleotide sequence of regulator: 810 bp    .

>REG0080 NC_003063:c2054864-2054055 [Agrobacterium fabrum str. C58]
ATCCTATCTATAGAGCTTCTTGAGGCGCAGTATCTTATCAATATGCGGATGGACGTCTATGCTTCTTCGACATCAAGTTT
TGTCGACAGCCTGCAAGGGATGGATCGTGCCTCTAAACAGGTGAGTTGTGAACGAGGGCGCGATTTGCCGCCGCACAGGC
CCAGCGATAATTAAAAAGGAAAAGAAGGCATGAAGGCAGCCGTTTACGATCAAGCAGGACCTCCGGATGTTTTGACGTAC
AGGGACGTCGCCGACCCGATTGTAGGTCCGGATGATGTCCTCATCGCAGTGGAAGCCATTTCGATTGAAGGAGGAGACTT
GATCAATCGTCGATCCACGCCGCCTCCTGGCCGCCCGTGGATAGTCGGCTATGCAGCATCTGGGCGCGTCGTGGGGGCCG
GTGCGAACGTGAGGGACCGCAAAGTCGGAGACAGGGTTACTGCCTTTGACATGCAGGGTTCGCACGCCGAACTCTGGGCC
GTGCCAGCGATCCGAACGTGGCTTTTGCCATCCGGCGTGGATGCAGCGTCGGCTGCCGCTTTGCCGATATCGTTTGGTAC
TGCCCACCATTGTCTTTTTGCCAGAGGTGGCCTTCTGCGCAACCAGACGGTTCTTGTACAGGCAGCGGCGGGTGGAGTTG
GCCTCGCCGCAGTTCAGCTCGCGGCTCAAGCCGGCGCAACCGTCATCGCCGTCTCAAGTGGAGAAAGCCGGCTGCAAAGG
ATATCTTCCCTTGGGGCTGATCACGTTGTCGATCGGTCGATGGGGAACGTTGTCGAGGCTGTCAGACAGAACACGGGAGG
CAAAGGAGTC