Detailed information of accessory protein


Summary

Accessory protein ID ACP00010 experimental
Type adaptor
Related T6SS (Type) T6SS00016 (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Related structure protein Atu4348 (VgrG1)
Related effector Atu4350 (Tde1)
Description Tap-1, DUF4123 domain-containing protein, is required for Tde1-dependent anti-bacterial activity and Tde1 secretion
Reference [1] Bondage DD, Lin JS, Ma LS, et al. VgrG C terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3931-40. doi: 10.1073/pnas.1600428113. Epub 2016 Jun 16. PMID: 27313214
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Atu4349 (tap-1)
Coordinates (Strand) 1483948..1484904 (+)
NCBI ID 737273750
RefSeq NC_003063
Uniprot ID UPI00054EBD36
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF4123 PF13503.8 6.1e-12 32..161
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-318 MNDTPIIDLPNPNETAELTRQAVEAMTGQVSVVLDGGAFDNLPRILQEEGISARSLFLEGASADFRLSGPWLVDLSAIPVRSYIGWLDAKNACAVYWSCPSGTDDLYRHLRTINEVMIPLEQTSGDLPATSSPQYERVLFRHWDPNVLGALLPILTRPQLARFLGPADCVVLNAPDYGGVKRVKAPADLPAAPPGPLRIERDQMEKLKAAMLHSSRLRIARFLKANTPPYFSGVDDNFIWGATLASEKSADELGIKTERGRARWAYVMIMSDGKAADAPEVRNYVGDGGDTPDNRVKSLIDHTVDALRSNPSASGAPA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9858 - -


  Protein sequence: 319 a.a.    

>ACP00010 NC_003063:1483948-1484904 [Agrobacterium fabrum str. C58]
MNDTPIIDLPNPNETAELTRQAVEAMTGQVSVVLDGGAFDNLPRILQEEGISARSLFLEGASADFRLSGPWLVDLSAIPV
RSYIGWLDAKNACAVYWSCPSGTDDLYRHLRTINEVMIPLEQTSGDLPATSSPQYERVLFRHWDPNVLGALLPILTRPQL
ARFLGPADCVVLNAPDYGGVKRVKAPADLPAAPPGPLRIERDQMEKLKAAMLHSSRLRIARFLKANTPPYFSGVDDNFIW
GATLASEKSADELGIKTERGRARWAYVMIMSDGKAADAPEVRNYVGDGGDTPDNRVKSLIDHTVDALRSNPSASGAPA*

  Nucleotide sequence: 957 bp    

>ACP00010 NC_003063:1483948-1484904 [Agrobacterium fabrum str. C58]
ATGAACGACACACCCATAATTGATCTGCCAAATCCGAACGAAACCGCCGAGCTTACAAGACAAGCCGTAGAGGCAATGAC
GGGACAGGTGTCCGTCGTTTTAGATGGGGGTGCATTTGATAATCTTCCGCGCATCCTTCAGGAAGAAGGTATAAGCGCCC
GGTCATTGTTTCTTGAAGGTGCTTCCGCCGATTTTCGTTTGTCGGGACCATGGTTAGTCGATCTCTCGGCTATCCCCGTG
CGCTCCTATATCGGCTGGCTAGACGCAAAAAATGCTTGCGCTGTCTATTGGTCCTGTCCGTCTGGAACCGATGATCTTTA
CCGTCACCTGAGGACTATTAACGAGGTTATGATCCCGCTGGAGCAGACCAGTGGCGATCTGCCAGCTACGAGTTCACCCC
AGTATGAACGCGTATTGTTTCGACACTGGGACCCTAATGTCCTCGGCGCTCTTCTTCCAATCCTGACAAGGCCACAGCTC
GCCCGGTTTTTAGGACCTGCGGATTGCGTCGTTTTAAACGCGCCCGACTATGGTGGTGTGAAGCGGGTGAAGGCTCCCGC
CGACCTACCTGCTGCTCCGCCTGGCCCGTTACGCATCGAACGGGATCAAATGGAAAAACTGAAAGCTGCGATGTTGCATT
CTTCTCGGCTGAGAATAGCCCGGTTCCTGAAAGCGAATACTCCACCGTATTTTTCTGGCGTCGATGATAACTTCATTTGG
GGCGCCACCTTGGCCAGCGAAAAATCTGCCGATGAGCTTGGTATCAAGACGGAACGCGGTCGCGCTCGCTGGGCCTATGT
CATGATAATGTCCGATGGAAAGGCGGCAGATGCACCGGAGGTGCGAAACTATGTTGGCGATGGCGGCGACACGCCAGATA
ACAGGGTTAAATCCCTGATCGACCACACCGTCGATGCCTTGCGTTCCAATCCGTCAGCATCCGGAGCGCCCGCATGA