SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01308
Effector type	Tde
Cognate immunity protein	-
Related T6SS (Type)	T6SS00016 (Type i5)
Strain	Agrobacterium fabrum str. C58
Replicon	chromosome
Sequence	Protein sequence (279 a.a.); Nucleotide sequence (837 bp)
Description	Tde1; DNase Effector
Reference	[1] Ma LS, Hachani A, Lin JS, et al. Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe. 2014 Jul 9;16(1):94-104. doi: 10.1016/j.chom.2014.06.002. Epub 2014 Jun 26. PMID: 24981331

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	Atu4350 (Tde1)
Coordinates (Strand)	1484901..1485737 (+)
NCBI ID	15890633
RefSeq	NC_003063
Uniprot ID	A9CGH1_AGRFC
KEGG ID	atu:Atu4350
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Ntox15	PF15604.8	2e-55	100..247

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-278	MSATTTVAGAGGAIAGGWLGRVIGGLAGSLAGPGGTAVGAWIGGQVGAMAGRAAASAIASYMEDANVDADAKTKESEQAKPCVDCGEIDCFSPPEGATPKQVEEFRRQLKEQQDEINRMEPDDLVRNIDKYRQQGRPTDDAANRRQSREDYRTDRTRELEEKYLSKGRNDYKEQAANDVAEEMKKLAATHTLDLVAGGDGSISGLGDKSINSSLGSQWKGRRSEQLRSHAKKAAEQKKKMNAKLEECKPEGGNDNSPDAETPDNGTGKGDGNPDVPVS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.4580	-	-

Protein sequence: 279 a.a. .

                                    >EFF01308 NC_003063:1484901-1485737 Type VI DNase and RNase Effector (Tde) [Agrobacterium fabrum str. C58]
MSATTTVAGAGGAIAGGWLGRVIGGLAGSLAGPGGTAVGAWIGGQVGAMAGRAAASAIASYMEDANVDADAKTKESEQAK
PCVDCGEIDCFSPPEGATPKQVEEFRRQLKEQQDEINRMEPDDLVRNIDKYRQQGRPTDDAANRRQSREDYRTDRTRELE
EKYLSKGRNDYKEQAANDVAEEMKKLAATHTLDLVAGGDGSISGLGDKSINSSLGSQWKGRRSEQLRSHAKKAAEQKKKM
NAKLEECKPEGGNDNSPDAETPDNGTGKGDGNPDVPVS*

                                

Nucleotide sequence: 837 bp .

                                    >EFF01308 NC_003063:1484901-1485737 Type VI DNase and RNase Effector (Tde) [Agrobacterium fabrum str. C58]
ATGAGTGCGACGACAACTGTCGCCGGAGCGGGCGGTGCTATCGCAGGAGGTTGGCTCGGTCGTGTTATTGGAGGCTTAGC
TGGCAGCCTCGCTGGGCCTGGAGGTACAGCGGTTGGAGCATGGATAGGTGGGCAAGTCGGCGCTATGGCGGGTAGAGCGG
CTGCCTCCGCAATCGCCTCTTATATGGAAGATGCGAATGTAGACGCTGACGCCAAGACCAAGGAGAGCGAACAGGCCAAA
CCTTGCGTTGATTGTGGGGAAATCGATTGCTTTAGTCCGCCTGAGGGAGCTACGCCTAAGCAAGTTGAAGAGTTTAGGCG
TCAGTTGAAGGAGCAACAAGACGAAATAAACCGCATGGAGCCTGATGATCTGGTCAGAAATATCGACAAATATAGACAGC
AGGGGAGGCCGACTGATGATGCAGCAAACCGTCGGCAGAGTAGAGAAGATTATCGCACCGACCGCACACGGGAACTGGAA
GAAAAGTATTTATCGAAAGGGCGCAATGACTATAAGGAACAGGCTGCGAATGATGTGGCAGAAGAGATGAAGAAGTTGGC
CGCAACCCATACCTTGGATTTGGTTGCGGGGGGCGACGGATCAATTTCTGGCTTGGGCGACAAGAGTATCAATAGTTCTT
TAGGGTCTCAATGGAAGGGAAGAAGATCGGAGCAATTGCGCTCTCATGCAAAAAAAGCGGCTGAGCAAAAGAAAAAAATG
AACGCTAAACTCGAAGAATGTAAACCCGAAGGCGGCAATGATAATTCTCCCGATGCTGAAACTCCCGACAATGGTACAGG
TAAGGGGGACGGCAATCCTGACGTCCCGGTGTCTTGA

                                