Detailed information of regulator


Summary

Regulator ID REG0085 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) Two-component system (map02020)
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (242 a.a.); Nucleotide sequence (726 bp)
Description The proteins encoded by the imp or hcp operon reduced with chvG or chvI deletion as determined by western blot and qRT-PCR analyses. ChvI can bind the T6SS promoter region revealed by EMSA binding and activate T6SS.
Reference
External database links

Locus tag (Gene) chvI
Coordinates (Strand) 36525..37250 (-)
NCBI ID
RefSeq NC_003062
Uniprot ID -
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
The intergenic region of the imp and hcp operons ↓ gagtagtctatccccagaaaatagtaatggattttttgtagttacttcccttacgtgaggctattaatcagacagacttattcaaaatgtgtctaaattgtggcgtctactccctaatctaatctgataaaaaataaacttcggggtggacaacccggtttcatcatcatagttattccacgtcacgttgtggaatgaacccgggcaggaggctcattgcatgtc
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 1.1e-27 4..113
Trans_reg_C PF00486.30 1.9e-21 161..237
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-241 MQTIALVDDDRNILTSVSIALEAEGYRVETYTDGASALDGLIARPPQLAIFDIKMPRMDGMELLRRLRQKSDIPVIFLTSKDEEIDELFGLKMGADDFITKPFSQRLLVERVKAILRRASSREASAATGGTLKPTADQQARTLERGQLAMDQERHTCTWKGEPVTLTVTEFLILHSLAQRPGVVKSRDALMDAAYDEQVYVDDRTIDSHIKRLRKKFKLVDGDFDMIETLYGVGYRFREAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9934 - -


  Protein sequence of regulator: 242 a.a.    .

>REG0085 NC_003062:c37250-36525 [Agrobacterium fabrum str. C58]
MQTIALVDDDRNILTSVSIALEAEGYRVETYTDGASALDGLIARPPQLAIFDIKMPRMDGMELLRRLRQKSDIPVIFLTS
KDEEIDELFGLKMGADDFITKPFSQRLLVERVKAILRRASSREASAATGGTLKPTADQQARTLERGQLAMDQERHTCTWK
GEPVTLTVTEFLILHSLAQRPGVVKSRDALMDAAYDEQVYVDDRTIDSHIKRLRKKFKLVDGDFDMIETLYGVGYRFREA
A*

  Nucleotide sequence of regulator: 726 bp    .

>REG0085 NC_003062:c37250-36525 [Agrobacterium fabrum str. C58]
ATGCAGACGATCGCGCTTGTCGACGATGACCGGAATATTCTCACTTCGGTATCGATCGCGCTCGAAGCCGAAGGCTACCG
GGTCGAAACGTATACGGACGGCGCCTCCGCGCTTGACGGGTTGATCGCCCGTCCGCCGCAGCTTGCCATTTTCGATATTA
AGATGCCCCGCATGGACGGCATGGAGCTTTTGCGACGCCTTCGCCAGAAATCGGATATTCCCGTTATCTTCCTCACCTCG
AAGGATGAGGAGATCGACGAATTGTTCGGCCTGAAGATGGGTGCAGACGATTTCATCACAAAGCCGTTTTCGCAGCGCCT
TCTGGTGGAGCGCGTCAAGGCCATCCTGCGCCGTGCCAGCAGCCGTGAGGCGTCCGCTGCAACCGGCGGCACGCTGAAGC
CGACCGCGGACCAGCAGGCGCGCACGCTGGAGCGTGGACAGCTCGCCATGGACCAGGAACGCCACACCTGCACCTGGAAG
GGTGAGCCGGTGACGCTGACAGTGACTGAATTCCTCATCCTGCATTCGCTTGCACAGCGGCCGGGCGTGGTGAAAAGCCG
CGACGCGTTGATGGATGCCGCCTATGACGAACAGGTCTATGTGGACGACCGCACCATCGACAGCCACATCAAGCGTCTTC
GCAAGAAGTTCAAACTGGTCGATGGCGACTTCGATATGATTGAAACGCTTTATGGCGTAGGATATCGCTTCCGCGAAGCG
GCTTAA