Detailed information of regulator
Summary
| Regulator ID | REG0085  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-RR |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020) |
| Related T6SS | T6SS00016 (Type i5) |
| Strain | Agrobacterium fabrum str. C58 |
| Replicon | chromosome |
| Sequence | Protein sequence (242 a.a.); Nucleotide sequence (726 bp) |
| Description | The proteins encoded by the imp or hcp operon reduced with chvG or chvI deletion as determined by western blot and qRT-PCR analyses. ChvI can bind the T6SS promoter region revealed by EMSA binding and activate T6SS. |
| Reference |
External database links
| Locus tag (Gene) | chvI |
| Coordinates (Strand) | 36525..37250 (-) |
| NCBI ID | |
| RefSeq | NC_003062 |
| Uniprot ID | - |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map02020 |
| PDB ID | - |
Target
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Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| The intergenic region of the imp and hcp operons | ↓ | gagtagtctatccccagaaaatagtaatggattttttgtagttacttcccttacgtgaggctattaatcagacagacttattcaaaatgtgtctaaattgtggcgtctactccctaatctaatctgataaaaaataaacttcggggtggacaacccggtttcatcatcatagttattccacgtcacgttgtggaatgaacccgggcaggaggctcattgcatgtc |
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Pfam domain hit(s) of regulator
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Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 1.1e-27 | 4..113 |
| Trans_reg_C | PF00486.30 | 1.9e-21 | 161..237 |
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Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
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Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-241 | MQTIALVDDDRNILTSVSIALEAEGYRVETYTDGASALDGLIARPPQLAIFDIKMPRMDGMELLRRLRQKSDIPVIFLTSKDEEIDELFGLKMGADDFITKPFSQRLLVERVKAILRRASSREASAATGGTLKPTADQQARTLERGQLAMDQERHTCTWKGEPVTLTVTEFLILHSLAQRPGVVKSRDALMDAAYDEQVYVDDRTIDSHIKRLRKKFKLVDGDFDMIETLYGVGYRFREAA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9934 | - | - |
Protein sequence of regulator: 242 a.a. .
>REG0085 NC_003062:c37250-36525 [Agrobacterium fabrum str. C58]
MQTIALVDDDRNILTSVSIALEAEGYRVETYTDGASALDGLIARPPQLAIFDIKMPRMDGMELLRRLRQKSDIPVIFLTS
KDEEIDELFGLKMGADDFITKPFSQRLLVERVKAILRRASSREASAATGGTLKPTADQQARTLERGQLAMDQERHTCTWK
GEPVTLTVTEFLILHSLAQRPGVVKSRDALMDAAYDEQVYVDDRTIDSHIKRLRKKFKLVDGDFDMIETLYGVGYRFREA
A*
MQTIALVDDDRNILTSVSIALEAEGYRVETYTDGASALDGLIARPPQLAIFDIKMPRMDGMELLRRLRQKSDIPVIFLTS
KDEEIDELFGLKMGADDFITKPFSQRLLVERVKAILRRASSREASAATGGTLKPTADQQARTLERGQLAMDQERHTCTWK
GEPVTLTVTEFLILHSLAQRPGVVKSRDALMDAAYDEQVYVDDRTIDSHIKRLRKKFKLVDGDFDMIETLYGVGYRFREA
A*
Nucleotide sequence of regulator: 726 bp .
>REG0085 NC_003062:c37250-36525 [Agrobacterium fabrum str. C58]
ATGCAGACGATCGCGCTTGTCGACGATGACCGGAATATTCTCACTTCGGTATCGATCGCGCTCGAAGCCGAAGGCTACCG
GGTCGAAACGTATACGGACGGCGCCTCCGCGCTTGACGGGTTGATCGCCCGTCCGCCGCAGCTTGCCATTTTCGATATTA
AGATGCCCCGCATGGACGGCATGGAGCTTTTGCGACGCCTTCGCCAGAAATCGGATATTCCCGTTATCTTCCTCACCTCG
AAGGATGAGGAGATCGACGAATTGTTCGGCCTGAAGATGGGTGCAGACGATTTCATCACAAAGCCGTTTTCGCAGCGCCT
TCTGGTGGAGCGCGTCAAGGCCATCCTGCGCCGTGCCAGCAGCCGTGAGGCGTCCGCTGCAACCGGCGGCACGCTGAAGC
CGACCGCGGACCAGCAGGCGCGCACGCTGGAGCGTGGACAGCTCGCCATGGACCAGGAACGCCACACCTGCACCTGGAAG
GGTGAGCCGGTGACGCTGACAGTGACTGAATTCCTCATCCTGCATTCGCTTGCACAGCGGCCGGGCGTGGTGAAAAGCCG
CGACGCGTTGATGGATGCCGCCTATGACGAACAGGTCTATGTGGACGACCGCACCATCGACAGCCACATCAAGCGTCTTC
GCAAGAAGTTCAAACTGGTCGATGGCGACTTCGATATGATTGAAACGCTTTATGGCGTAGGATATCGCTTCCGCGAAGCG
GCTTAA
ATGCAGACGATCGCGCTTGTCGACGATGACCGGAATATTCTCACTTCGGTATCGATCGCGCTCGAAGCCGAAGGCTACCG
GGTCGAAACGTATACGGACGGCGCCTCCGCGCTTGACGGGTTGATCGCCCGTCCGCCGCAGCTTGCCATTTTCGATATTA
AGATGCCCCGCATGGACGGCATGGAGCTTTTGCGACGCCTTCGCCAGAAATCGGATATTCCCGTTATCTTCCTCACCTCG
AAGGATGAGGAGATCGACGAATTGTTCGGCCTGAAGATGGGTGCAGACGATTTCATCACAAAGCCGTTTTCGCAGCGCCT
TCTGGTGGAGCGCGTCAAGGCCATCCTGCGCCGTGCCAGCAGCCGTGAGGCGTCCGCTGCAACCGGCGGCACGCTGAAGC
CGACCGCGGACCAGCAGGCGCGCACGCTGGAGCGTGGACAGCTCGCCATGGACCAGGAACGCCACACCTGCACCTGGAAG
GGTGAGCCGGTGACGCTGACAGTGACTGAATTCCTCATCCTGCATTCGCTTGCACAGCGGCCGGGCGTGGTGAAAAGCCG
CGACGCGTTGATGGATGCCGCCTATGACGAACAGGTCTATGTGGACGACCGCACCATCGACAGCCACATCAAGCGTCTTC
GCAAGAAGTTCAAACTGGTCGATGGCGACTTCGATATGATTGAAACGCTTTATGGCGTAGGATATCGCTTCCGCGAAGCG
GCTTAA