Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00044 experimental
T6SS type Type i5
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon (RefSeq) chromosome II (NC_004605)
Location 1074208..1108114
Function anti-eukaryotic; effector translocation; adherence
Comment Adhesion to cultured cell monolayers.
References
[1] Yu Y et al (2012) VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2. Wei Sheng Wu Xue Bao. 52(8):954-61. [PudMed:23173431]
[2] Yu Y et al (2015) VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 0.366666667. [PudMed:25784905]
[3] Ma L et al (2012) Expression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR in Vibrio parahaemolyticus. Scientific World Journal. 2012:982140. [PudMed:22924031]
[4] Yu Y et al (2012) Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 194(10):827-35. [PudMed:22535222]
[5] Salomon D et al (2013) Vibrio parahaemolyticus Type VI Secretion System 1 Is Activated in Marine Conditions to Target Bacteria, and Is Differentially Regulated from System 2. PLoS One. 8(4):e61086. [PudMed:23613791]
[6] Wang L et al (2013) Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus. PLoS One. 8(8):e73363. [PudMed:23977385]
[7] Boyer F et al (2009) Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 0.488888889. [PudMed:19284603]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPAARTagJTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111121111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
VP_RS20045 1069624..1070757 - 1134 WP_005480347.1 multidrug effflux MFS transporter
VP_RS20050 1070897..1071676 + 780 WP_011106404.1 helix-turn-helix transcriptional regulator
VP_RS20055 1071695..1072216 - 522 WP_005463837.1 lipocalin family protein
VP_RS20060 1072272..1072799 - 528 WP_005463834.1 phosphoglycerate mutase family protein
VP_RS20065 1072830..1073354 - 525 WP_005480324.1 AAA family ATPase
VP_RS20070 1073763..1074143 + 381 WP_005480328.1 pyruvate dehydrogenase
VP_RS20075 1074208..1077264 - 3057 WP_005480361.1 SMC family ATPase
VP_RS20080 1077274..1078407 - 1134 WP_005480232.1 exonuclease SbcCD subunit D
VP_RS20085 1078504..1079346 - 843 WP_005480244.1 hypothetical protein
T6CP011352 PAAR VP_RS20090 1079347..1079637 - 291 WP_005499010.1 PAAR domain-containing protein
T6CP000366 TssI VP_RS20095 (tssI) 1079651..1081486 - 1836 WP_005480263.1 type VI secretion system tip protein VgrG
T6CP000367 TssD VP_RS20100 1081544..1082023 - 480 WP_005375967.1 type VI secretion system tube protein Hcp
T6CP000368 TssH VP_RS20105 (tssH) 1082229..1084796 - 2568 WP_005480296.1 type VI secretion system ATPase TssH
T6CP000369 TssG VP_RS20110 (tssG) 1084807..1085772 - 966 WP_005480294.1 type VI secretion system baseplate subunit TssG
T6CP000370 TssF VP_RS20115 (tssF) 1085769..1087592 - 1824 WP_005463819.1 type VI secretion system baseplate subunit TssF
T6CP000371 TssE VP_RS20120 (tssE) 1087589..1088053 - 465 WP_005463817.1 type VI secretion system baseplate subunit TssE
T6CP012705 TagJ VP_RS20125 1088062..1088859 - 798 WP_005463815.1 protein of avirulence locus
T6CP000372 TssC VP_RS20130 (tssC) 1088870..1090381 - 1512 WP_005480259.1 type VI secretion system contractile sheath large subunit
T6CP000373 TssC VP_RS20135 (tssC) 1090437..1091930 - 1494 WP_005463811.1 type VI secretion system contractile sheath large subunit
T6CP000374 TssB VP_RS20140 (tssB) 1091930..1092460 - 531 WP_005463809.1 type VI secretion system contractile sheath small subunit
T6CP009338 TssA VP_RS20145 (tssA) 1092476..1093597 - 1122 WP_005480330.1 type VI secretion system protein TssA
VP_RS20150 1093594..1094382 - 789 WP_005463805.1 protein phosphatase 2C domain-containing protein
VP_RS20155 (tagF) 1094393..1095088 - 696 WP_005463803.1 type VI secretion system-associated protein TagF
T6CP000375 TssM VP_RS20160 (tssM) 1095070..1098588 - 3519 WP_005463801.1 type VI secretion system membrane subunit TssM
T6CP000376 TssL VP_RS20165 (tssL) 1098604..1099896 - 1293 WP_005463799.1 type VI secretion system protein TssL, long form
T6CP000377 TssK VP_RS20170 (tssK) 1099908..1101233 - 1326 WP_005463797.1 type VI secretion system baseplate subunit TssK
T6CP000378 TssJ VP_RS20175 (tssJ) 1101255..1101710 - 456 WP_005463795.1 type VI secretion system lipoprotein TssJ
T6CP011353 FHA VP_RS20180 (tagH) 1101720..1102916 - 1197 WP_005463793.1 type VI secretion system-associated FHA domain protein TagH
VP_RS20185 1103239..1105380 + 2142 WP_005480257.1 serine/threonine protein kinase
VP_RS20190 1105492..1107027 + 1536 WP_005463789.1 response regulator
VP_RS20195 1107030..1107365 + 336 WP_005463787.1 hypothetical protein
VP_RS20200 1107401..1107592 + 192 WP_005480239.1 hypothetical protein
VP_RS20205 1107620..1108114 - 495 WP_005480282.1 ATP-binding protein
VP_RS20210 1108107..1109327 - 1221 WP_005463782.1 SpoIIE family protein phosphatase
VP_RS20215 1109352..1109612 - 261 WP_005463779.1 hypothetical protein
VP_RS20220 1109834..1110448 - 615 WP_005463777.1 glutathione S-transferase
VP_RS20225 1110593..1111168 + 576 WP_005463775.1 TetR/AcrR family transcriptional regulator
VP_RS20230 1111170..1111574 + 405 WP_005463773.1 DUF296 domain-containing protein
VP_RS20235 (nirD) 1111677..1112012 - 336 WP_005463771.1 nitrite reductase small subunit NirD
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0100 experimental VP_RS12495 (barA) protein TCS-HK Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2712492..2715290 (+) 491625032 Hcp expression and secretion (↓)
REG0101 experimental VP_RS09470 (UvrY) protein TCS-RR Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2037468..2038112 (-) 491529158 CsrB (↓)
REG0102 experimental VP_RS12395 (CsrA) protein carbon storage regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2688313..2688510 (-) 490550575 Hcp and vgrG expression and secretion (↓)
REG0103 experimental CsrB1 ncRNA carbon storage regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2438728..2439035 (+) - VP_RS12395 (CsrA) (↑)
REG0104 experimental CsrB2 ncRNA carbon storage regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 3215766..3216162 (-) - VP_RS12395 (CsrA) (↑)
REG0105 experimental CsrB3 ncRNA carbon storage regulatory pathway Vibrio parahaemolyticus RIMD 2210633 plasmid (NC_004605) 171473..171785 (+) - VP_RS12395 (CsrA) (↑)
REG0111 experimental VP2762 (AphA) protein QS regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2929909..2930448 (+) 28899536 T6SS1 transcript (↑); T6SS2 (VPA1027-1024, VPA1043-1028,VPA1044-1046) transcripts (↑)
REG0112 experimental VP2516 (opaR) protein QS regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 2653215..2653829 (+) 28899290 vp1407 (↑); VP1388 promotor (↑); VP1400 promotor (↑); VP1409 promotor (↑); T6SS2 (Hcp2) expression and secretion (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1027-1024 transcript (↓); VPA1043-1028 transcript (↓); VPA1044-1046 transcript (↓)
REG0115 experimental VP0350 (CalR/LeuO) protein Transcription regulator Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 344763..345722 (+) 28897124 VPA1027 (hcp2) promotor (↓); VPA1043 (Fha) promotor (↓); VP1133 (H-NS) (↓)
REG0119 experimental VP1241 (TfoX) protein QS regulatory pathway; c-di-GMP regulatory pathway Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004603) 1309223..1309810 (+) 28898016 T6SS2 (↓); T6SS1 (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00061 experimental VP_RS20100 - - Vibrio parahaemolyticus RIMD 2210633 chromosome (NC_004605) 1081544..1082023 (-) 28900882

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector