SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0111
Regulator type	protein
Regulatory pathway (from literatures)	QS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111)
Related T6SS	T6SS00043 (Type i1); T6SS00044 (Type i5)
Strain	Vibrio parahaemolyticus RIMD 2210633
Replicon	chromosome
Sequence	Protein sequence (180 a.a.); Nucleotide sequence (540 bp)
Description	AphA appears to negatively regulate the transcription of T6SS2 in an indirect manner.; AphA appears to negatively regulate the transcription of VPA1027-1024, VPA1043-1028, and VPA1044-1046 in an indirect manner.
Reference	[1] Zhang Y, Gao H, Osei-Adjei G, et al. Transcriptional Regulation of the Type VI Secretion System 1 Genes by Quorum Sensing and ToxR in Vibrio parahaemolyticus. Front Microbiol. 2017 Oct 16;8:2005. doi: 10.3389/fmicb.2017.02005. eCollection 2017. PMID: 29085350 [2] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385

External database links

Locus tag (Gene)	VP2762 (AphA)
Coordinates (Strand)	2929909..2930448 (+)
NCBI ID	28899536
RefSeq	NC_004603
Uniprot ID	Q87L53_VIBPA
KEGG ID	vpa:VP2762
KEGG pathway (determined by BlastKOALA)	map02024; map05111
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS1 transcript	↑	-
T6SS2 (VPA1027-1024, VPA1043-1028,VPA1044-1046) transcripts	↑	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
PadR	PF03551.16	1.2e-15	7..80
Vir_act_alpha_C	PF10400.11	4.6e-15	92..176

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-179	MSLPHVILTVLSTRDATGYDITKEFSASIGYFWKASHQQVYRELNKMAEKELVTCVLEPQEGKPDRKVYSITDAGRSALGEWFDQPTAHPTVRDEFSAKLMACAVQPSTPFRDQLAELVEESRKLVSHYKEIEAAYYATPSTLDKQARLERLTLRRNLLLRETWITWAEEVLTELEVIG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9045	-	-

Protein sequence of regulator: 180 a.a. .

                                    >REG0111 NC_004603:2929909-2930448 [Vibrio parahaemolyticus RIMD 2210633]
MSLPHVILTVLSTRDATGYDITKEFSASIGYFWKASHQQVYRELNKMAEKELVTCVLEPQEGKPDRKVYSITDAGRSALG
EWFDQPTAHPTVRDEFSAKLMACAVQPSTPFRDQLAELVEESRKLVSHYKEIEAAYYATPSTLDKQARLERLTLRRNLLL
RETWITWAEEVLTELEVIG*

                                 

Nucleotide sequence of regulator: 540 bp .

                                    >REG0111 NC_004603:2929909-2930448 [Vibrio parahaemolyticus RIMD 2210633]
ATGTCATTACCACACGTAATCTTAACTGTTCTTAGCACTCGCGACGCAACCGGTTACGATATCACTAAAGAATTCTCTGC
TAGCATCGGTTACTTCTGGAAAGCAAGCCACCAACAAGTTTACCGTGAACTAAATAAAATGGCCGAGAAAGAGCTGGTTA
CTTGCGTACTAGAACCTCAAGAAGGCAAACCAGACCGTAAGGTTTACTCCATCACAGATGCAGGCCGTAGCGCTCTTGGA
GAATGGTTTGATCAACCAACTGCACACCCAACCGTTCGTGATGAATTTTCAGCGAAACTTATGGCTTGTGCTGTTCAACC
ATCAACACCTTTCCGTGATCAACTAGCAGAACTTGTTGAAGAGTCTCGTAAACTGGTTTCTCACTACAAAGAAATTGAAG
CTGCTTACTACGCAACGCCTTCAACTTTGGACAAACAAGCTCGTTTAGAGCGCCTAACGCTTCGTCGTAACCTACTGTTA
CGTGAAACGTGGATCACTTGGGCAGAAGAAGTCCTAACAGAACTTGAAGTGATTGGTTAA

                                