Detailed information of component protein


Summary

Component ID T6CP000368
Component protein type TssH
T6SS ID (Type) T6SS00044 experimental (Type i5)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome II
Sequence Protein sequence (856 a.a.); Nucleotide sequence (2568 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] [Article in Chinese] [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2]. Wei Sheng Wu Xue Bao. 2012 Aug 4;52(8):954-61. PMID: 23173431
[6] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385
[7] Yu Y, Fang L, Zhang Y, et al. VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 2015 Mar 2;6:168. doi: 10.3389/fmicb.2015.00168. eCollection 2015. PMID: 25784905
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VP_RS20105
Coordinate (Strand) 1082229..1084796 (-)
NCBI ID WP_005480296.1
RefSeq NC_004605
Uniprot ID Q87HD7_VIBPA
KEGG ID vpa:VPA1028
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 1.3e-38 588..753
AAA_lid_9 PF17871.3 7.6e-27 353..444
ClpB_D2-small PF10431.11 7.7e-10 760..833
AAA PF00004.31 2e-09 213..326
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-855 MASLSLSALVERLSPQAKETLEQAAAMASSRTHHSIELTHWLLTIVQHPSGIYSEVFEAFDIDERKVEEQLQAQLEKYKTGCQTVPGLSAHTVTIMQNALMCATIEFHEEQVNCIHLLFAYLSDDTLVNMMNAISAELDKVEPSRLVVMKEHVGDGGNNTSKATSSSNSALNQFTTDLTEQAKAGKIDPVIGRDNEIRLMIDILLRRRQNNPILTGEAGVGKTAVIEGLAQKIVDGDVPPALHDVAIKSLDLALLQAGAGMKGEFENRLKNLIREVNESSQPIILFIDEAHGLIGGGGQAGQGDAANILKPALARGELRTIAATTWAEYKKYFEKDAALTRRFQVVKVDEPTPELAVDMLRGLVNIMGQHHDVYITSQALNAAVQLSARYINGRQLPDKAVSVLDTACSRVALSQSATPGALDSKKKKLNIKEAEFQQLTKEAQLGVSHDELLNNIRYEIDELSNQVIELEAIWEQEKQWVQEAKSLRARVIEENADCDKERLQEIESNLALNAQPLVFSHVNESLIAEVISDWTGIPLGKLQDDEIEAVLNLKDSLCERVVGQDHALEIMSEVIKTASAQLTDETKPNGVFLLTGPSGVGKTESALAIAEKVYGSEDNVTTINMSEFKEEHKVSLLLGSPPGYVGYGEGGVLTEAVRRKPYSVILLDEMEKAHPGVQDIFYQVFDKGSIKDGEGRDIDFKNTIIIMTANTGTETTMSLYADPDLAPEPEALKEALRDDLLTDFKPAFLGRVNVLPYLPLGKEVLTEITKLKLNKVSNRIRKHYSAGLYFDEQLINDIVDNSNESGSGARVVQTTIENNLLPKISHEILNAILNGKTFDEINVRGSIKELEVSLI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9788 - -


  Protein sequence: 856 a.a.    

>T6CP000368 NC_004605:c1084796-1082229 [Vibrio parahaemolyticus RIMD 2210633] [TssH]
MASLSLSALVERLSPQAKETLEQAAAMASSRTHHSIELTHWLLTIVQHPSGIYSEVFEAFDIDERKVEEQLQAQLEKYKT
GCQTVPGLSAHTVTIMQNALMCATIEFHEEQVNCIHLLFAYLSDDTLVNMMNAISAELDKVEPSRLVVMKEHVGDGGNNT
SKATSSSNSALNQFTTDLTEQAKAGKIDPVIGRDNEIRLMIDILLRRRQNNPILTGEAGVGKTAVIEGLAQKIVDGDVPP
ALHDVAIKSLDLALLQAGAGMKGEFENRLKNLIREVNESSQPIILFIDEAHGLIGGGGQAGQGDAANILKPALARGELRT
IAATTWAEYKKYFEKDAALTRRFQVVKVDEPTPELAVDMLRGLVNIMGQHHDVYITSQALNAAVQLSARYINGRQLPDKA
VSVLDTACSRVALSQSATPGALDSKKKKLNIKEAEFQQLTKEAQLGVSHDELLNNIRYEIDELSNQVIELEAIWEQEKQW
VQEAKSLRARVIEENADCDKERLQEIESNLALNAQPLVFSHVNESLIAEVISDWTGIPLGKLQDDEIEAVLNLKDSLCER
VVGQDHALEIMSEVIKTASAQLTDETKPNGVFLLTGPSGVGKTESALAIAEKVYGSEDNVTTINMSEFKEEHKVSLLLGS
PPGYVGYGEGGVLTEAVRRKPYSVILLDEMEKAHPGVQDIFYQVFDKGSIKDGEGRDIDFKNTIIIMTANTGTETTMSLY
ADPDLAPEPEALKEALRDDLLTDFKPAFLGRVNVLPYLPLGKEVLTEITKLKLNKVSNRIRKHYSAGLYFDEQLINDIVD
NSNESGSGARVVQTTIENNLLPKISHEILNAILNGKTFDEINVRGSIKELEVSLI*

  Nucleotide sequence: 2568 bp    

>T6CP000368 NC_004605:c1084796-1082229 [Vibrio parahaemolyticus RIMD 2210633] [TssH]
ATGGCTTCATTATCACTTTCAGCGTTGGTCGAAAGATTGTCTCCGCAAGCGAAGGAAACGCTAGAGCAAGCGGCTGCAAT
GGCAAGTTCGCGTACACATCATAGTATAGAGTTGACCCACTGGTTGTTGACGATAGTTCAACATCCATCCGGTATTTATT
CAGAAGTGTTTGAAGCTTTTGATATTGATGAAAGAAAAGTCGAAGAACAACTGCAAGCTCAGCTAGAAAAATACAAAACG
GGATGCCAAACCGTCCCAGGCCTTTCTGCTCATACTGTAACCATTATGCAGAATGCTTTGATGTGCGCAACGATAGAGTT
TCATGAAGAACAAGTGAACTGTATCCACTTACTGTTTGCGTATCTTTCTGATGACACGCTAGTGAATATGATGAACGCTA
TTAGCGCGGAGCTAGACAAAGTCGAACCATCTCGCCTAGTCGTAATGAAAGAGCATGTGGGCGATGGTGGAAACAATACT
TCTAAGGCGACGAGCAGCTCGAACTCTGCATTGAATCAGTTCACAACGGATCTTACTGAGCAAGCTAAAGCTGGAAAAAT
AGATCCTGTTATCGGTCGCGACAATGAAATCCGCTTGATGATCGATATCTTATTGCGTCGCCGCCAAAACAATCCGATCT
TAACGGGTGAGGCAGGCGTAGGTAAAACTGCGGTAATTGAGGGGCTGGCGCAAAAAATAGTGGATGGGGATGTTCCTCCT
GCTTTACATGATGTTGCCATCAAATCACTCGATCTTGCTTTGCTTCAGGCGGGTGCGGGGATGAAAGGGGAGTTTGAAAA
TAGACTTAAAAATCTCATTCGAGAAGTGAACGAATCTTCGCAGCCAATCATTCTTTTTATCGATGAAGCTCATGGTCTAA
TCGGAGGTGGTGGTCAAGCGGGACAAGGTGATGCTGCTAATATATTGAAACCAGCGTTGGCTCGAGGAGAGTTACGGACC
ATCGCTGCAACTACTTGGGCTGAGTACAAAAAATATTTTGAAAAAGATGCTGCGTTAACGAGGCGCTTCCAGGTTGTGAA
AGTAGACGAGCCGACTCCTGAGTTAGCGGTAGATATGCTTAGAGGCTTGGTTAACATCATGGGGCAACATCATGATGTCT
ATATCACAAGCCAAGCACTGAATGCTGCGGTACAACTTTCTGCTCGCTACATTAACGGACGCCAACTGCCAGATAAAGCA
GTATCGGTATTAGATACAGCATGTTCGCGTGTTGCATTGAGCCAATCTGCCACGCCGGGCGCGTTGGATAGCAAAAAGAA
AAAGCTAAACATCAAAGAAGCTGAGTTTCAACAACTTACCAAAGAGGCTCAATTGGGCGTAAGTCACGATGAACTGCTTA
ATAATATTCGGTATGAGATCGATGAGTTATCTAATCAAGTCATAGAATTAGAGGCAATTTGGGAACAAGAAAAGCAGTGG
GTTCAAGAAGCTAAGTCGTTGAGAGCTAGGGTGATAGAAGAGAATGCCGACTGTGACAAAGAGCGATTACAGGAAATCGA
ATCGAACTTAGCGCTCAACGCCCAACCTTTAGTCTTTTCTCATGTGAATGAATCTTTGATTGCTGAGGTGATTTCTGATT
GGACAGGCATTCCACTTGGAAAATTGCAAGATGACGAAATTGAAGCGGTTCTTAACCTAAAAGATAGCTTGTGCGAGCGA
GTGGTGGGGCAAGATCATGCTCTAGAAATCATGTCCGAAGTGATCAAAACAGCAAGTGCCCAATTGACGGATGAAACCAA
ACCTAATGGCGTGTTTTTATTAACTGGCCCTAGTGGTGTCGGAAAAACGGAATCCGCTCTTGCTATCGCAGAGAAAGTTT
ATGGGAGCGAAGACAACGTAACCACGATAAATATGTCGGAATTCAAAGAAGAGCATAAGGTTTCATTGTTGTTAGGCTCT
CCACCTGGATATGTCGGATACGGTGAGGGGGGCGTTCTAACGGAAGCCGTAAGGCGTAAACCTTACAGTGTGATTCTGTT
GGATGAAATGGAAAAAGCGCACCCCGGTGTGCAAGATATTTTCTATCAAGTGTTTGATAAAGGCTCCATTAAAGATGGTG
AAGGTCGAGATATCGATTTTAAAAACACCATCATTATCATGACGGCGAATACTGGCACAGAAACCACAATGTCTCTCTAT
GCAGATCCAGATTTAGCGCCAGAACCGGAAGCTTTAAAAGAAGCGCTTAGAGATGACTTGCTAACAGATTTTAAACCTGC
ATTTTTGGGGCGGGTTAATGTATTACCTTACTTGCCTCTCGGAAAAGAAGTCCTAACGGAAATAACTAAGTTAAAACTCA
ACAAAGTATCTAACCGTATCCGAAAACATTATTCGGCAGGGTTGTATTTTGATGAACAACTAATTAATGACATTGTAGAC
AATAGTAATGAGTCAGGAAGTGGTGCTCGTGTAGTTCAAACCACCATTGAAAATAATTTACTTCCTAAAATTTCTCATGA
AATATTGAATGCAATTCTTAATGGTAAGACGTTCGATGAAATTAATGTTAGAGGTTCAATTAAAGAACTAGAAGTCTCAC
TTATTTAA