Detailed information of component protein


Summary

Component ID T6CP000372
Component protein type TssC
T6SS ID (Type) T6SS00044 experimental (Type i5)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome II
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] [Article in Chinese] [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2]. Wei Sheng Wu Xue Bao. 2012 Aug 4;52(8):954-61. PMID: 23173431
[6] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385
[7] Yu Y, Fang L, Zhang Y, et al. VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 2015 Mar 2;6:168. doi: 10.3389/fmicb.2015.00168. eCollection 2015. PMID: 25784905
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VP_RS20130
Coordinate (Strand) 1088870..1090381 (-)
NCBI ID WP_005480259.1
RefSeq NC_004605
Uniprot ID A0A7T1BNE2_VIBPH
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 2e-106 67..376
VipB_2 PF18945.2 1.2e-41 388..497
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-503 MTKIDALNFLDPEELTHEFTPMGLAEFDSHWLTQFVECSDSILAARIWLEGTQSKDFASFRHSIVSTINTIDTLLQKQLNEIIHHPDFQKLECSWVGVRYLCEQVDKPSSDSVKIKVLSATWNEVSKDALKAIEFDQSALFKLLYQNEYGMAGGEPFGVVVGDYQLRYDPRTNYFDRDISVLSKISQSAAAAFSPFVMSASPEIFGVNTFAELSSTRDVAAQFEQIDYVKWRQLRDNDDTKFIGLTAPNVLFRQPYKSDGSRNDQFEFQENIDDSNNDLLWGSGAFCFAAVSIRAFQEHGWFTHMRGIKQGDYSQGIIVAPTRNKTRINGKNDRDRCPLNLKVSERKEQELSDCGFIPISPVPETDMVGMTSNVSLYKPKLYEEKHVATNAKLTSMLQYTMCVSRIAHYVKVMGRDKIGGYQDAASLEREFQTWLHQYTTASDEASDELRAKYPLNEAKIKVREKRDTPGHFYSVIHLRPHFQLDQMVSSIKLITELSPEHLV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9968 - -


  Protein sequence: 504 a.a.    

>T6CP000372 NC_004605:c1090381-1088870 [Vibrio parahaemolyticus RIMD 2210633] [TssC]
MTKIDALNFLDPEELTHEFTPMGLAEFDSHWLTQFVECSDSILAARIWLEGTQSKDFASFRHSIVSTINTIDTLLQKQLN
EIIHHPDFQKLECSWVGVRYLCEQVDKPSSDSVKIKVLSATWNEVSKDALKAIEFDQSALFKLLYQNEYGMAGGEPFGVV
VGDYQLRYDPRTNYFDRDISVLSKISQSAAAAFSPFVMSASPEIFGVNTFAELSSTRDVAAQFEQIDYVKWRQLRDNDDT
KFIGLTAPNVLFRQPYKSDGSRNDQFEFQENIDDSNNDLLWGSGAFCFAAVSIRAFQEHGWFTHMRGIKQGDYSQGIIVA
PTRNKTRINGKNDRDRCPLNLKVSERKEQELSDCGFIPISPVPETDMVGMTSNVSLYKPKLYEEKHVATNAKLTSMLQYT
MCVSRIAHYVKVMGRDKIGGYQDAASLEREFQTWLHQYTTASDEASDELRAKYPLNEAKIKVREKRDTPGHFYSVIHLRP
HFQLDQMVSSIKLITELSPEHLV*

  Nucleotide sequence: 1512 bp    

>T6CP000372 NC_004605:c1090381-1088870 [Vibrio parahaemolyticus RIMD 2210633] [TssC]
ATGACGAAAATAGATGCATTGAACTTTCTAGACCCAGAGGAACTCACACACGAGTTTACACCGATGGGCCTGGCTGAGTT
TGACAGTCATTGGTTGACTCAATTTGTTGAATGTTCAGATTCAATACTTGCGGCACGAATTTGGTTGGAAGGCACGCAAT
CTAAAGATTTTGCGAGTTTTCGCCATAGCATTGTGAGCACGATTAATACCATTGACACACTATTACAGAAGCAGCTCAAT
GAGATCATTCATCATCCAGATTTTCAGAAATTAGAGTGCAGTTGGGTAGGCGTTCGATATTTATGTGAACAAGTTGATAA
ACCATCGTCTGACTCCGTAAAAATTAAAGTTCTATCTGCAACATGGAATGAAGTAAGTAAAGATGCGCTCAAGGCTATCG
AGTTCGATCAGTCTGCCTTGTTTAAATTGCTCTATCAAAATGAATATGGAATGGCCGGAGGAGAACCCTTTGGTGTGGTT
GTTGGTGATTATCAATTACGTTATGACCCGAGAACAAATTATTTTGATAGAGATATCTCTGTGCTAAGCAAGATATCTCA
GTCTGCTGCTGCGGCATTTAGTCCATTTGTAATGTCTGCGTCTCCTGAAATATTCGGTGTGAACACATTTGCCGAACTTT
CGTCGACTCGAGACGTTGCGGCACAATTTGAACAAATTGATTATGTCAAATGGAGACAGTTGCGAGATAACGATGATACC
AAGTTTATTGGCTTAACCGCTCCTAACGTGCTTTTTCGACAACCCTACAAAAGTGATGGTTCTCGAAACGATCAATTTGA
ATTTCAAGAGAATATTGACGATTCAAATAACGATTTATTGTGGGGAAGTGGAGCCTTCTGTTTTGCTGCTGTTTCGATTA
GAGCATTCCAAGAGCACGGTTGGTTTACTCACATGCGAGGAATAAAACAGGGGGATTATAGTCAAGGCATTATTGTTGCT
CCTACAAGAAACAAAACGCGCATTAATGGCAAAAATGACAGAGATAGATGCCCTTTAAATCTAAAAGTCTCTGAGCGTAA
AGAGCAAGAACTGTCTGATTGTGGGTTCATTCCGATTTCACCAGTTCCTGAAACAGACATGGTCGGCATGACTTCCAATG
TTTCGTTGTATAAGCCAAAACTGTACGAAGAGAAGCATGTTGCGACTAATGCAAAACTCACCTCAATGCTCCAATACACG
ATGTGTGTATCGCGTATAGCACACTACGTCAAAGTTATGGGGCGAGACAAGATAGGTGGCTATCAGGACGCGGCATCGCT
CGAGAGGGAGTTTCAGACTTGGTTACATCAATATACAACGGCTTCAGACGAAGCTTCGGATGAACTCAGAGCTAAGTACC
CATTGAACGAAGCGAAGATCAAAGTTAGAGAGAAACGCGATACGCCAGGGCACTTCTACTCGGTGATACATTTACGCCCT
CATTTTCAGCTCGACCAAATGGTCTCAAGTATCAAATTAATTACGGAATTATCACCAGAACATTTGGTGTAA