Detailed information of regulator
|Regulatory pathway (from literatures)||TCS-RR|
|KEGG pathway (determined by BlastKOALA)||Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)|
|Related T6SS||T6SS00043 (Type i1); T6SS00044 (Type i5)|
|Strain||Vibrio parahaemolyticus RIMD 2210633|
|Sequence||Protein sequence (215 a.a.); Nucleotide sequence (645 bp)|
|Description||A ChIP‐qPCR confirmed by examination of the selected candidates from ChIP‐Seq that UvrY at least binds to the promoter regions of three ncRNA (CsrB1, CsrB2, and CsrB3),and the gene of potential T6SS1 regulator VP1407.Deletion of uvrY significantly reduced CsrB expression level in vitro when bacteria were cultured in LB medium at 23℃, suggesting that UvrY positively control CrsBs in vitro. Western blot analysis showed that the expression and secretion of effectors from 34 T6SS1 (Hcp1), and T6SS2 (Hcp2)75 were significantly decreased in ΔuvrY, and these defects were fully recovered by a complementation of ΔuvrY with WT copy of uvrY.|
External database links
|Locus tag (Gene)||VP_RS09470 (UvrY)|
|Coordinates (Strand)||2037468..2038112 (-)|
|Uniprot ID||S5IWM2_VIBPH, Q87NC3_VIBPA, A0A0T7E7L8_9VIBR, A0A0G9M1V6_VIBAL, D0WZF4_VIBAL|
|KEGG ID||vpa:VP1945, vpf:M634_11955|
|KEGG pathway (determined by BlastKOALA)||map02020; map05111; map02025; map02026|
|Target||Regulation (↓/↑)||Target sequence|
Pfam domain hit(s) of regulator
|Domain||Pfam ID||E-value||Aligned region|
- Transmembrane helices are predicted using TMHMM 2.0 software.
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
|Prediction||Probability||Cleavage site||Signal peptide sequence|
Protein sequence of regulator: 215 a.a. .
Nucleotide sequence of regulator: 645 bp .