Detailed information of component protein


Summary

Component ID T6CP000375
Component protein type TssM
T6SS ID (Type) T6SS00044 experimental (Type i5)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome II
Sequence Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] [Article in Chinese] [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2]. Wei Sheng Wu Xue Bao. 2012 Aug 4;52(8):954-61. PMID: 23173431
[6] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385
[7] Yu Y, Fang L, Zhang Y, et al. VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 2015 Mar 2;6:168. doi: 10.3389/fmicb.2015.00168. eCollection 2015. PMID: 25784905
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VP_RS20160
Coordinate (Strand) 1095070..1098588 (-)
NCBI ID WP_005463801.1
RefSeq NC_004605
Uniprot ID Q87HC6_VIBPA
KEGG ID vpa:VPA1039
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 8.5e-86 193..450
IcmF-related PF06761.14 3.7e-76 504..802
IcmF_C PF06744.14 3.6e-18 1049..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MSIKEIGRVFTQ
Transmembrane helix 13-32 RWFLGLVGVAACSIFIWVVG
Outside 33-46 PLITVAGYEPLKSD
Transmembrane helix 47-64 FQRLVTILVIVFAWALIN
Inside 65-445 LTKQHKQKVREDESIQTLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLTESLGADSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHARSIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAHMLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGRLPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRT
Transmembrane helix 446-468 GIYVGCVGVLVGASALWFLSYQW
Outside 469-1172 NSKLIVDTNSQVNHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESLVSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATAISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQKEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWMTNVVRPYQRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6736 - -


  Protein sequence: 1173 a.a.    

>T6CP000375 NC_004605:c1098588-1095070 [Vibrio parahaemolyticus RIMD 2210633] [TssM]
MSIKEIGRVFTQRWFLGLVGVAACSIFIWVVGPLITVAGYEPLKSDFQRLVTILVIVFAWALINLTKQHKQKVREDESIQ
TLLEVDSQSDKEAASEIDVMRDRIEQAIKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLTESLGADSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTVSLASVMSQTRTERSLHAR
SIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAH
MLRILETEDDLEKRTLIFEFPKQLRVLQANLDEFLSEIFAQNTFEEPALIRGVFLLSSVQEGIPVDRLMSESTNGLGLGR
LPLATNVNSSHSYFVKNLFERVIFKEQLLGTVNRHYQKQSGWMRTGIYVGCVGVLVGASALWFLSYQWNSKLIVDTNSQV
NHIEAMIGAESLDFESDVISAVDTLDKIMMLPLGKNSKYGHSDAVKKFGLYQGDKVSQAANNAYSDALSQHFATLLSESL
VSEMEANKQHREYLYETLKTYLMLFNPEKYQQEEVIAWFNFYFERQYPGELNKELRERLLVHTKNLLENDEKGFSMDATA
ISAAREVLTQMSLPERAYQRMKMQFAKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KGLMEENWVYGDDLKAHEIDHDSAIQGVQARYYQDYVNEWKTLIEDIQLKQAPSLALATEQSRVLSGVERPIESLLRAIQ
KEVGLSKVTLSENQKAATEVAGKVAKVKFSNTADKLDMYLPEENGFNVALPGKEVESHFTEILRLSEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKDLLPKPFNQWLGELSQESVKFAESGSKDHLNQLWMTNVVRPY
QRTIAGRYPFEPNATKEVRLKDFQRFFGYGGTLDSFFQEYLEPFVDTSKSRWRLEKEIGVRPETLAVFQRAKRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGDQDLVYKHGPARSKELRWPSGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRNDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPQTL*

  Nucleotide sequence: 3519 bp    

>T6CP000375 NC_004605:c1098588-1095070 [Vibrio parahaemolyticus RIMD 2210633] [TssM]
ATGAGTATTAAAGAAATAGGCAGAGTATTTACCCAAAGATGGTTTCTTGGTTTAGTCGGTGTAGCCGCGTGCTCCATTTT
TATTTGGGTTGTGGGTCCTCTGATCACGGTTGCAGGTTACGAGCCGCTTAAAAGTGATTTTCAACGTTTAGTTACGATCT
TAGTGATTGTTTTTGCTTGGGCGCTTATCAACTTAACAAAGCAGCACAAACAGAAAGTTAGAGAAGACGAAAGTATTCAG
ACGTTGCTTGAAGTAGACAGCCAATCAGACAAAGAAGCCGCCAGTGAAATTGACGTAATGCGCGATCGTATAGAGCAAGC
GATCAAAGTTGTGACAAAAACGCATAAAGGAAAGCGTAGTTTGTACGATTTACCTTGGTACGTTCTGATTGGTCCTCCTG
GAACAGGTAAAACCACGGTTCTAAAGCAATCTGGCTTGGAATTTCCGTTAACTGAGAGCTTAGGAGCTGACTCTATAGCT
GGTGTAGGTGGAACACGTCACTGTGATTGGTGGTTTGCTAATAAAGCAGTACTTATTGATACAGCAGGACGTTACACCAC
GCAAGATAGTCAAGAAAAGGTTGACTCTAAAGCTTGGCACGGTTTCTTAGGGTTGCTTAAGAAATACCGCACTCAAAGGC
CTATCAATGGCGCTATCGTAACAGTCAGCCTAGCAAGTGTGATGTCTCAAACTCGTACGGAGCGTAGCTTACATGCCAGA
TCAATAAAATCTCGCCTTCAGGAACTCAAAAATCAGTTGGGTATGCAATTCCCAATCTATGTTCTGCTGACGAAAATGGA
TCTGGTTGCTGGCTTTAATGAATTTTTTGCTGATTTAAGTAAAGAAGAGCGTGAGGAACTTTTTGGATTTATGTTCCCAC
GAGAAGTTGATGACGAACGTGGTGTTATTTCTCTATTCAATAAAGAGTTTCACGGCATGTTGGAGCGGCTCGATGCGCAC
ATGTTACGTATATTAGAAACTGAAGATGACTTAGAAAAGCGCACGCTAATATTCGAATTTCCAAAACAGCTGCGAGTTTT
ACAAGCAAACTTAGATGAGTTTTTGTCTGAGATATTTGCGCAGAACACGTTTGAAGAGCCAGCCCTTATTCGCGGTGTTT
TCTTGCTTAGCTCTGTACAAGAAGGTATTCCTGTTGATCGACTGATGAGTGAATCAACTAATGGATTGGGACTAGGGAGG
TTGCCTTTAGCGACAAACGTTAACAGTTCGCATAGCTATTTTGTTAAAAATCTTTTTGAGCGAGTCATCTTTAAAGAGCA
ATTACTAGGTACTGTTAACCGCCACTATCAAAAGCAATCTGGATGGATGCGTACGGGCATATACGTGGGTTGTGTTGGTG
TTCTTGTTGGTGCTTCTGCTCTTTGGTTCCTGAGTTATCAATGGAATTCAAAGTTAATTGTCGATACCAATAGCCAAGTA
AATCATATTGAAGCGATGATAGGTGCAGAGAGCCTTGATTTTGAATCGGACGTTATCAGTGCTGTAGACACGTTAGACAA
GATCATGATGTTGCCACTAGGGAAAAATAGTAAATATGGTCATTCTGATGCAGTGAAAAAGTTTGGTCTGTATCAAGGTG
ACAAAGTTTCTCAAGCAGCAAATAACGCGTATTCAGATGCTCTTTCTCAACACTTTGCTACTCTGCTGTCAGAGAGTTTG
GTCTCGGAAATGGAAGCGAATAAGCAGCATCGTGAATACTTATACGAGACGTTAAAGACGTATTTAATGTTGTTTAATCC
GGAAAAGTATCAACAAGAAGAGGTAATTGCTTGGTTCAACTTCTACTTTGAACGTCAGTATCCAGGAGAGTTAAACAAAG
AGCTACGTGAGCGTTTGCTAGTGCATACTAAAAACTTACTAGAGAACGACGAGAAAGGCTTCTCAATGGATGCGACTGCT
ATTAGTGCAGCACGAGAAGTTCTAACGCAAATGTCACTTCCAGAGCGTGCATATCAGCGTATGAAGATGCAGTTTGCTAA
AAGCCACGTTCCGTCTTTCCGTTTAACAGACGTGTTAGGACCTAAAGGTTTAGAACAATTTGAGCGAGCAAGTGGAAAAC
CTCTATCGCAAGGTATTTCCGGTTTTTATACCTACAACGGTTTTCACAGCATTTTCCAAATTCAGATTAATCGCACTGTC
AAAGGATTGATGGAAGAAAACTGGGTGTATGGGGATGATCTTAAAGCTCATGAAATTGATCATGATAGTGCTATTCAGGG
GGTTCAAGCCCGCTATTATCAAGATTATGTCAATGAGTGGAAAACTCTGATAGAGGATATTCAGCTTAAACAAGCTCCAA
GTTTGGCTTTAGCAACAGAGCAATCAAGAGTTTTATCCGGTGTAGAAAGACCAATTGAATCACTTCTGCGTGCAATACAA
AAGGAAGTGGGGTTATCGAAAGTAACGTTATCGGAAAATCAAAAGGCAGCAACGGAAGTCGCAGGAAAGGTTGCAAAAGT
GAAGTTTTCTAATACGGCAGACAAACTCGATATGTATTTGCCTGAAGAGAATGGATTTAATGTTGCTCTACCAGGTAAAG
AAGTTGAATCCCATTTCACCGAAATCTTACGACTTAGTGAACAAGACTTTGATGATATTCAGCAAGCGATGGTGAATTTG
CGTTCGTACTTATCTGATTTGTCTAGCTCTGGCAACAATCAAAAAATTGCTTACAAAAGCATATTGGATGGAACGGTAAC
TCAGGATGTTGCTGCCTCCTTTGCTAGAGCAAAAGATCTCTTACCAAAACCGTTTAATCAATGGTTGGGAGAGTTGTCAC
AGGAATCGGTTAAGTTTGCAGAAAGCGGCTCTAAAGATCATCTAAATCAACTTTGGATGACTAATGTAGTGCGTCCTTAT
CAGCGGACTATAGCAGGTAGATACCCATTTGAACCTAATGCAACGAAGGAAGTTCGTCTAAAGGACTTTCAACGATTCTT
CGGGTACGGTGGTACGTTGGACTCGTTCTTCCAAGAATATCTGGAGCCGTTTGTTGATACGTCTAAGAGTCGTTGGAGAC
TAGAAAAAGAAATCGGTGTAAGGCCCGAGACGCTTGCGGTTTTTCAAAGGGCAAAACGCATTAGACAATCGTTCTTTGAA
TCAGATAACTCGCTACGAGTCGAGTTTGGTATGAAACCCGTATATCTAGATCAACACATTACTCGATTTGTTCTAGAGCT
AGGTGATCAAGACTTGGTTTATAAGCACGGTCCTGCGAGATCGAAGGAACTTCGCTGGCCATCGGGTCAAGATCAGACGC
GTATTGTGTTTACACCGCCCGAATCAAAACGAGAAATTGCTCATACCTATGAAGGGGAATGGGGCATTTTCAAACTATTG
GACCAATCACTCAAGGCTCGACCAGAGTCCCGCAACGACAACATTGTCATGATCGATTTGAAGGGGAACAAGGTTCAGCT
TGAGTTGATTCCATCTAGTGCGATCAACCCATTTTGGTCTAATGAGATGGAGAGGTTCAGATGTCCGCAAACCTTGTAA