Detailed information of component protein


Summary

Component ID T6CP000376
Component protein type TssL
T6SS ID (Type) T6SS00044 experimental (Type i5)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome II
Sequence Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] [Article in Chinese] [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2]. Wei Sheng Wu Xue Bao. 2012 Aug 4;52(8):954-61. PMID: 23173431
[6] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385
[7] Yu Y, Fang L, Zhang Y, et al. VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 2015 Mar 2;6:168. doi: 10.3389/fmicb.2015.00168. eCollection 2015. PMID: 25784905
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VP_RS20165
Coordinate (Strand) 1098604..1099896 (-)
NCBI ID WP_005463799.1
RefSeq NC_004605
Uniprot ID UPI0001564399
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.1e-55 50..252
OmpA PF00691.22 4.7e-13 322..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-229 MEQTIVKPTPGGRAAVSKAQPQRSADSTVVISKNPELVNNDSVVAYGDNPLLAEANGLLSIIGQIRATATHSDPLFLKETLAQKLRDYENRLRQHDVDLETIDTARYCLCCSLDEAVLNTNWGSQSFWTHDSLLSSFYASSQGGEAFFKHLDSCLAHPESHLDLLELMYVCLSLGFIGQYRLEKNGLEAHRRLRKQVVSVLKSHGRGVQQELSNKVEQHILAGAQVSER
Transmembrane helix 230-252 APLWVVCSVTSALLVCIFMYFSY
Inside 253-430 ELNKASNQTFAQLVNLIQPTPAVSNPMVESKSAPIAERISMYLATEIGKDLVTVEALQDRVRISLKAQDLFESGSASVVAYIQPVISKVARTLEATQGKIIITGHTDDRPIFTSKYPSNWHLSLARATSLSEQLISNSALKGRVIPEGLGDARPLVENDSEKNRAMNRRIEIDLIVGN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9378 - -


  Protein sequence: 431 a.a.    

>T6CP000376 NC_004605:c1099896-1098604 [Vibrio parahaemolyticus RIMD 2210633] [TssL]
MEQTIVKPTPGGRAAVSKAQPQRSADSTVVISKNPELVNNDSVVAYGDNPLLAEANGLLSIIGQIRATATHSDPLFLKET
LAQKLRDYENRLRQHDVDLETIDTARYCLCCSLDEAVLNTNWGSQSFWTHDSLLSSFYASSQGGEAFFKHLDSCLAHPES
HLDLLELMYVCLSLGFIGQYRLEKNGLEAHRRLRKQVVSVLKSHGRGVQQELSNKVEQHILAGAQVSERAPLWVVCSVTS
ALLVCIFMYFSYELNKASNQTFAQLVNLIQPTPAVSNPMVESKSAPIAERISMYLATEIGKDLVTVEALQDRVRISLKAQ
DLFESGSASVVAYIQPVISKVARTLEATQGKIIITGHTDDRPIFTSKYPSNWHLSLARATSLSEQLISNSALKGRVIPEG
LGDARPLVENDSEKNRAMNRRIEIDLIVGN*

  Nucleotide sequence: 1293 bp    

>T6CP000376 NC_004605:c1099896-1098604 [Vibrio parahaemolyticus RIMD 2210633] [TssL]
ATGGAACAGACGATAGTAAAACCTACTCCTGGCGGAAGAGCGGCTGTATCTAAAGCTCAGCCTCAAAGATCAGCAGACAG
TACTGTTGTGATATCCAAAAACCCTGAGTTGGTTAATAACGATTCAGTTGTTGCTTATGGAGATAATCCACTATTGGCAG
AAGCTAATGGTCTACTTTCGATTATTGGACAAATCAGAGCAACAGCTACTCATTCCGATCCTTTGTTTCTTAAAGAAACG
CTCGCGCAAAAACTTAGGGATTATGAGAATCGTCTGCGTCAACATGATGTTGACTTGGAAACCATTGATACCGCACGTTA
TTGTCTGTGTTGTTCTTTAGACGAAGCAGTATTGAATACCAATTGGGGAAGTCAATCGTTTTGGACACATGATTCTCTGT
TGAGTTCTTTTTACGCGAGCAGCCAAGGTGGAGAGGCTTTTTTTAAGCATCTAGATAGCTGTTTGGCTCATCCGGAATCC
CACCTAGATTTACTAGAGCTAATGTACGTATGCTTATCTTTGGGTTTTATAGGTCAGTATCGTTTAGAAAAAAATGGGCT
TGAAGCTCACCGTAGATTGAGAAAACAAGTTGTATCAGTGCTTAAGTCACATGGTCGCGGAGTACAGCAAGAGCTTAGTA
ACAAAGTAGAACAACATATATTGGCAGGTGCTCAGGTTTCGGAACGTGCTCCTCTATGGGTGGTTTGTTCGGTAACTTCC
GCACTGCTTGTTTGTATTTTTATGTACTTCAGCTACGAGTTGAACAAAGCTTCCAACCAAACTTTTGCTCAATTGGTAAA
CCTGATTCAACCAACCCCAGCAGTTAGTAATCCTATGGTTGAGTCAAAGTCAGCACCTATTGCCGAAAGAATATCTATGT
ATTTGGCAACGGAGATTGGCAAGGATCTTGTCACTGTGGAAGCGTTGCAAGATAGAGTTCGTATTTCTCTTAAAGCTCAA
GACCTATTTGAATCTGGTAGTGCGAGCGTTGTGGCTTACATACAGCCAGTTATTTCTAAAGTTGCACGCACCTTAGAAGC
GACACAAGGGAAGATCATAATTACCGGGCATACCGATGACCGTCCGATATTTACCAGCAAGTACCCATCCAATTGGCACT
TGTCTTTAGCTCGGGCAACTTCTCTTTCCGAACAGTTAATCTCCAACAGTGCACTTAAAGGAAGAGTGATTCCTGAAGGG
TTGGGTGATGCGAGACCTTTGGTAGAAAATGATAGTGAAAAGAACCGAGCAATGAATCGTCGAATTGAAATCGATCTTAT
TGTGGGCAACTAA