Detailed information of component protein


Summary

Component ID T6CP000373
Component protein type TssC
T6SS ID (Type) T6SS00044 experimental (Type i5)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome II
Sequence Protein sequence (498 a.a.); Nucleotide sequence (1494 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] [Article in Chinese] [VPA1045 and VPA1049 of Vibrio parahaemolyticus regulate translocation of Hcp2]. Wei Sheng Wu Xue Bao. 2012 Aug 4;52(8):954-61. PMID: 23173431
[6] Wang L, Zhou D, Mao P, et al. Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. PLoS One. 2013 Aug 15;8(8):e73363. doi: 10.1371/journal.pone.0073363. eCollection 2013. PMID: 23977385
[7] Yu Y, Fang L, Zhang Y, et al. VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages. Front Microbiol. 2015 Mar 2;6:168. doi: 10.3389/fmicb.2015.00168. eCollection 2015. PMID: 25784905
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) VP_RS20135
Coordinate (Strand) 1090437..1091930 (-)
NCBI ID WP_005463811.1
RefSeq NC_004605
Uniprot ID S5ITZ3_VIBPH, Q87HD1_VIBPA, A0A0D1RGP9_VIBPH
KEGG ID vpa:VPA1034, vpf:M634_23090
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 1.8e-129 64..372
VipB_2 PF18945.2 1.1e-43 383..492

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-497 MTTQVEKQLDPTVGEQTTSFLEQAIGATKQTEASRAEELIKTLTEEAMKGTVSWNKNLTVTFREAINLLDRQISEQLSEVMHHPELQKLEGSWRGLNYLVMNSETSSTLKIRMISITKKELHKDLSKAVEFDQSQIFKKVYESEFGSAGGEPYGALIGDYEFTNHPEDIESLRLMSNVAASGFSPFLSAASPALFGFDEWTELSKPRDLDKVFESLEYAQWRSFRESADSRFVSLTMPKVLARLPYGQATSPVEAFGFEEFDVDPVSGIAVNADHNDYCWMNSSYVLGVKLTDAFSKYGFCTAIRGAEGGGRVDNLPTHFFMSDDGDPDMKCPTEIGITDRREAELGKLGFLPLCHYKNTNYAVFFGAQTCQKPANHESPEVAANAAISARLPYMMATSRFAHYLKVMARDKIGSFMEAEDVESWLNRWILGYVNASEGGGQEIRAKYPLADARVQVKEIPGSPGSYNAVAWLKPWLQMEELTTSLRLVAKIPQSGG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9904 - -



  Protein sequence: 498 a.a.    

>T6CP000373 NC_004605:c1091930-1090437 [Vibrio parahaemolyticus RIMD 2210633] [TssC]
MTTQVEKQLDPTVGEQTTSFLEQAIGATKQTEASRAEELIKTLTEEAMKGTVSWNKNLTVTFREAINLLDRQISEQLSEV
MHHPELQKLEGSWRGLNYLVMNSETSSTLKIRMISITKKELHKDLSKAVEFDQSQIFKKVYESEFGSAGGEPYGALIGDY
EFTNHPEDIESLRLMSNVAASGFSPFLSAASPALFGFDEWTELSKPRDLDKVFESLEYAQWRSFRESADSRFVSLTMPKV
LARLPYGQATSPVEAFGFEEFDVDPVSGIAVNADHNDYCWMNSSYVLGVKLTDAFSKYGFCTAIRGAEGGGRVDNLPTHF
FMSDDGDPDMKCPTEIGITDRREAELGKLGFLPLCHYKNTNYAVFFGAQTCQKPANHESPEVAANAAISARLPYMMATSR
FAHYLKVMARDKIGSFMEAEDVESWLNRWILGYVNASEGGGQEIRAKYPLADARVQVKEIPGSPGSYNAVAWLKPWLQME
ELTTSLRLVAKIPQSGG*

  Nucleotide sequence: 1494 bp    

>T6CP000373 NC_004605:c1091930-1090437 [Vibrio parahaemolyticus RIMD 2210633] [TssC]
ATGACGACTCAAGTTGAAAAACAACTCGATCCTACGGTGGGTGAACAAACCACTTCATTTCTTGAACAGGCTATTGGTGC
GACGAAACAAACAGAAGCATCGAGAGCAGAAGAGCTGATCAAGACACTGACTGAAGAAGCGATGAAGGGGACGGTTTCTT
GGAATAAAAACCTAACCGTAACTTTCCGTGAAGCGATTAATTTGCTTGATAGACAGATTTCAGAGCAGCTTTCTGAAGTT
ATGCACCATCCTGAGCTACAAAAGTTAGAAGGCTCATGGCGTGGGCTGAATTACTTAGTCATGAATTCTGAGACAAGTTC
TACGCTGAAAATTCGCATGATCAGCATCACGAAGAAAGAGCTTCATAAAGACTTAAGTAAAGCGGTTGAATTCGATCAAA
GTCAAATCTTTAAAAAAGTGTACGAATCTGAATTTGGTAGCGCTGGTGGTGAACCGTATGGTGCTTTAATTGGTGACTAC
GAGTTTACAAACCATCCAGAAGATATTGAGTCTTTGCGTTTAATGTCGAATGTTGCTGCTTCGGGTTTCTCACCATTCTT
GTCTGCTGCTTCTCCTGCGCTATTTGGTTTTGATGAGTGGACAGAACTATCTAAACCGCGCGATTTAGATAAAGTCTTTG
AGTCGTTGGAATATGCGCAATGGCGCTCTTTCCGTGAAAGTGCAGATTCTCGTTTTGTAAGCTTAACGATGCCTAAAGTA
TTAGCTCGCTTACCATACGGCCAAGCTACCTCTCCTGTAGAAGCGTTTGGCTTTGAAGAGTTTGATGTGGATCCTGTGTC
GGGTATCGCCGTTAATGCTGATCACAATGATTATTGTTGGATGAACTCATCGTACGTGCTTGGCGTCAAACTGACGGATG
CATTTTCTAAGTATGGTTTCTGTACTGCTATTCGTGGCGCAGAGGGTGGTGGGCGAGTTGATAACCTACCAACACACTTC
TTCATGAGTGATGATGGTGACCCAGATATGAAGTGCCCAACGGAAATTGGCATTACAGACCGCCGTGAAGCGGAATTGGG
TAAACTGGGTTTCTTACCATTGTGTCACTACAAAAATACGAACTATGCCGTGTTCTTTGGTGCTCAAACTTGTCAAAAAC
CAGCAAATCATGAATCTCCAGAAGTGGCAGCAAACGCGGCAATTTCAGCAAGACTTCCTTACATGATGGCAACCTCTCGT
TTTGCACATTATTTGAAAGTAATGGCTCGAGACAAGATCGGTAGCTTTATGGAAGCAGAAGACGTTGAATCTTGGTTGAA
CCGTTGGATTCTTGGCTATGTGAACGCTTCTGAAGGTGGCGGTCAAGAAATTCGTGCGAAATACCCTCTTGCCGACGCTC
GCGTACAAGTTAAGGAAATTCCAGGTTCTCCTGGTTCTTACAACGCGGTTGCTTGGCTCAAGCCATGGCTTCAAATGGAA
GAGCTAACCACGTCTTTAAGACTCGTTGCTAAAATTCCTCAGTCGGGTGGCTAA