Detailed information of regulator
Summary
| Regulator ID | REG0115  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Transcription regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00044 (Type i5) |
| Strain | Vibrio parahaemolyticus RIMD 2210633 |
| Replicon | chromosome |
| Sequence | Protein sequence (320 a.a.); Nucleotide sequence (960 bp) |
| Description | CalR activates the promoter activities of T6SS2 genes,and antagonise H-NS. |
| Reference |
External database links
| Locus tag (Gene) | VP0350 (CalR/LeuO) |
| Coordinates (Strand) | 344763..345722 (+) |
| NCBI ID | 28897124 |
| RefSeq | NC_004603 |
| Uniprot ID | Q87SS2_VIBPA |
| KEGG ID | vpa:VP0350 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| VPA1027 (hcp2) promotor | ↓ | tgcttcacggtccattgctgtacccgtgtaagaagaaatttcacgaccaacactccaatctacagatagtagtgtaattaaatccttatattgttccgctgtcgcttcacctttaatatctgcgtatttaagatacgtattagactgcatgctaatctcctagagcattattaattgacatatttcgtattaattaaattatctgaataatgataattaatgattgatgtatcaaaaacttcattttaaatacgctcctttatatctttaaattagatttaaattaagcgatatttataactaataatagttattatgaagcaactctcaattattatttacagagttaaacttgttaaataagtgagacttctagttctttaattgaacctctaacattaatttcatcgaacgtcttaccattaagaattgcattcaatatttcatgagaaattttaggaagtaaattattttcaatggtggtttgaactacacgagcaccacttcctgactcattactattgtctacaatgtcattaattagttgttcatcaaaatacaaccctgccgaataatgttttcggatacggttagatactttgttgagttttaacttagttatttccgttaggacttcttttccgagaggcaagtaaggtaata |
| VPA1043 (Fha) promotor | ↓ | tgagcgtccgaaggttacctttgcatgctcatcacttgattcaaacacaaactcactctctatttccggagtaaatttgtggaaactcgttatggtcaataccaatttcatgaaaaacctcgccaaagacctgctaaatatttatatatcgatttttataattagatttgaagctttaattattaacattaagattaatagagttcttatagctgttataaaaactattgacggcataatgaatcatttttttatcaaagcaatcatgaattgttgaacaattttaaatatccaaccaggttcaaatttttgacactcaaactttcacccgtcaaaaaaccgatgttagtttggccgagaaaatctaaccgaagtatttttcatttagcaggaatatatatattaaccccatcatttatatggagtccttgtgatggaagaaaaaaataaagacaaatctataaatagcagcgatagcggagataaaacaaaaattgtaaagactcaaaaaagagaaaatgcaattgaacccgctaaaaacgttaataaacaagactccgctgaaaatataaatgaaacggatacaaaactgtctcaaaaagaaaacacaataccacctatcaacaaa |
| VP1133 (H-NS) | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LysR_substrate | PF03466.22 | 1.1e-23 | 119..307 |
| HTH_1 | PF00126.29 | 5.2e-18 | 25..83 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-319 | MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPKIMAKINQQAPNVQLHLDAEFDRLLSERMRYQEIDFVIDYARFDEQGFSSTEIFKDELVVIASKTHPRIQGGVSAEQLINEKHAKLSKVHGQRSFSEQAYRELDCQAAYEGSSLSNLLYVVSQSELVTIAPRWMAENAVNSDQLQILDFPFANKEISGFLSWHESSEKDKGHIWLRDQLMVTCGEVVASK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9881 | - | - |
Protein sequence of regulator: 320 a.a. .
>REG0115 NC_004603:344763-345722 [Vibrio parahaemolyticus RIMD 2210633]
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPT
QRARQLFGPIRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPKIMAKINQQAPNVQLHLDAEFDRLLSERMRY
QEIDFVIDYARFDEQGFSSTEIFKDELVVIASKTHPRIQGGVSAEQLINEKHAKLSKVHGQRSFSEQAYRELDCQAAYEG
SSLSNLLYVVSQSELVTIAPRWMAENAVNSDQLQILDFPFANKEISGFLSWHESSEKDKGHIWLRDQLMVTCGEVVASK*
MLEKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPT
QRARQLFGPIRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPKIMAKINQQAPNVQLHLDAEFDRLLSERMRY
QEIDFVIDYARFDEQGFSSTEIFKDELVVIASKTHPRIQGGVSAEQLINEKHAKLSKVHGQRSFSEQAYRELDCQAAYEG
SSLSNLLYVVSQSELVTIAPRWMAENAVNSDQLQILDFPFANKEISGFLSWHESSEKDKGHIWLRDQLMVTCGEVVASK*
Nucleotide sequence of regulator: 960 bp .
>REG0115 NC_004603:344763-345722 [Vibrio parahaemolyticus RIMD 2210633]
ATGTTAGAGAAGAAAGATGCGATGAGCGCAATTGCTAGCTACCGTATGGAAAGCACTTTGCGTGGTGTAGATTTGAACCT
ACTGACTGTATTTGATGCTGTGATGCAAGAGCAAAATATCACTCGTGCAGCACACAACTTAGGCATGTCTCAACCGGCAG
TAAGTAATGCCGTGGCACGTCTAAAAGTGATGTTTAACGATGAACTGTTCATGCGTCAGGGCCGTGGTATTCAACCAACT
CAACGTGCGCGTCAACTGTTTGGCCCTATCCGCCAAGCATTGCAACTGATCCGTAACGAATTGCCAAGCTCGGTATTCCA
ACCAGAGTCGTCAACTCGTCTATTCAAGCTGGCGATTTGCAGCCCATGTGACATGCGCTTTGCTCCAAAAATCATGGCGA
AGATTAATCAACAAGCGCCAAACGTTCAGCTACATCTAGACGCGGAATTTGACCGTCTTCTTTCTGAGCGCATGCGTTAC
CAAGAAATCGACTTCGTGATCGATTACGCTCGTTTTGATGAGCAAGGTTTCTCAAGCACAGAAATTTTCAAAGATGAATT
GGTGGTTATCGCTTCGAAAACGCACCCACGTATTCAAGGTGGCGTAAGCGCCGAGCAGCTAATTAACGAAAAGCACGCGA
AACTGTCTAAAGTTCACGGTCAACGTAGCTTCTCTGAGCAGGCGTACCGCGAGCTTGACTGCCAAGCTGCGTACGAAGGT
TCAAGCCTAAGCAACTTGCTTTACGTCGTTAGCCAATCTGAGTTAGTGACTATCGCACCACGCTGGATGGCGGAAAATGC
GGTGAACAGTGACCAACTGCAGATTCTAGACTTCCCATTTGCGAACAAAGAAATCAGCGGCTTCCTTAGCTGGCATGAAT
CAAGTGAAAAAGACAAAGGCCACATTTGGCTACGTGACCAGCTAATGGTGACGTGTGGTGAAGTGGTCGCATCAAAATAA
ATGTTAGAGAAGAAAGATGCGATGAGCGCAATTGCTAGCTACCGTATGGAAAGCACTTTGCGTGGTGTAGATTTGAACCT
ACTGACTGTATTTGATGCTGTGATGCAAGAGCAAAATATCACTCGTGCAGCACACAACTTAGGCATGTCTCAACCGGCAG
TAAGTAATGCCGTGGCACGTCTAAAAGTGATGTTTAACGATGAACTGTTCATGCGTCAGGGCCGTGGTATTCAACCAACT
CAACGTGCGCGTCAACTGTTTGGCCCTATCCGCCAAGCATTGCAACTGATCCGTAACGAATTGCCAAGCTCGGTATTCCA
ACCAGAGTCGTCAACTCGTCTATTCAAGCTGGCGATTTGCAGCCCATGTGACATGCGCTTTGCTCCAAAAATCATGGCGA
AGATTAATCAACAAGCGCCAAACGTTCAGCTACATCTAGACGCGGAATTTGACCGTCTTCTTTCTGAGCGCATGCGTTAC
CAAGAAATCGACTTCGTGATCGATTACGCTCGTTTTGATGAGCAAGGTTTCTCAAGCACAGAAATTTTCAAAGATGAATT
GGTGGTTATCGCTTCGAAAACGCACCCACGTATTCAAGGTGGCGTAAGCGCCGAGCAGCTAATTAACGAAAAGCACGCGA
AACTGTCTAAAGTTCACGGTCAACGTAGCTTCTCTGAGCAGGCGTACCGCGAGCTTGACTGCCAAGCTGCGTACGAAGGT
TCAAGCCTAAGCAACTTGCTTTACGTCGTTAGCCAATCTGAGTTAGTGACTATCGCACCACGCTGGATGGCGGAAAATGC
GGTGAACAGTGACCAACTGCAGATTCTAGACTTCCCATTTGCGAACAAAGAAATCAGCGGCTTCCTTAGCTGGCATGAAT
CAAGTGAAAAAGACAAAGGCCACATTTGGCTACGTGACCAGCTAATGGTGACGTGTGGTGAAGTGGTCGCATCAAAATAA