Detailed information of regulator
Summary
| Regulator ID | REG0145  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-HK |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00077  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (615 a.a.); Nucleotide sequence (1845 bp) |
| Description | Low Molecular Weight Thiols Upregulate T6SS1 Expression through the VirA Sensor.Use Burkholderia pseudomallei K96243 gene description as Burkholderia pseudomallei strain KHW gene annotation. |
| Reference | [1] Wong J, Chen Y, Gan YH. Host Cytosolic Glutathione Sensing by a Membrane Histidine Kinase Activates the Type VI Secretion System in an Intracellular Bacterium. Cell Host Microbe. 2015 Jul 8;18(1):38-48. doi: 10.1016/j.chom.2015.06.002. Epub 2015 Jun 18. PMID: 26094804 [2] Chen Y, Wong J, Sun GW, et al. Regulation of type VI secretion system during Burkholderia pseudomallei infection. Infect Immun. 2011 Aug;79(8):3064-73. doi: 10.1128/IAI.05148-11. Epub 2011 Jun 13. PMID: 21670170 |
External database links
| Locus tag (Gene) | BPSS1495 (virA) |
| Coordinates (Strand) | 2038711..2040555 (-) |
| NCBI ID | 53722517 |
| RefSeq | NC_006351 |
| Uniprot ID | Q63K70_BURPS |
| KEGG ID | bps:BPSS1495 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| hcp1 expression | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| HATPase_c | PF02518.28 | 3.1e-18 | 454..592 |
| MASE1 | PF05231.16 | 5.2e-14 | 63..323 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-32 | MHGNSIDAGPPARARPARITRTARAAPRRADR |
| Transmembrane helix | 33-52 | AFAPGGALNAAGLLLLLFYA |
| Outside | 53-66 | NHLLSRPGACTSLN |
| Transmembrane helix | 67-86 | HTLSAVVWPAPVLGIALLLH |
| Inside | 87-92 | CRRPLA |
| Transmembrane helix | 93-115 | WWLGAGSLFATLLAAGSLDWVPW |
| Outside | 116-118 | HVD |
| Transmembrane helix | 119-141 | ALFAAVNVVEAIAGAWLARRYLI |
| Inside | 142-153 | AGGGVDTLRGFA |
| Transmembrane helix | 154-176 | MFVLLLPLGMAAMHATLAAAILS |
| Outside | 177-190 | VWMNDREWLSEWSR |
| Transmembrane helix | 191-213 | AYAANALALLVLLPALLTWNARA |
| Inside | 214-224 | CGATWSRSRNQ |
| Transmembrane helix | 225-247 | LPVLATFGSLCFASIWGYQAEVA |
| Outside | 248-261 | RALLALSLSWAALE |
| Transmembrane helix | 262-284 | GGLVLASQLNALSAIALIAMTLG |
| Inside | 285-296 | GRGPYAWHHDGH |
| Transmembrane helix | 297-319 | GVWALQVDLIGVAILSMCIAIAT |
| Outside | 320-614 | GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8347 | - | - |
Protein sequence of regulator: 615 a.a. .
>REG0145 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243]
MHGNSIDAGPPARARPARITRTARAAPRRADRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGATWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT*
MHGNSIDAGPPARARPARITRTARAAPRRADRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGATWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEASMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGVGSCFRFTLPAAWGTGAPGAMDAGAPGAVQERT*
Nucleotide sequence of regulator: 1845 bp .
>REG0145 NC_006351:c2040555-2038711 [Burkholderia pseudomallei K96243]
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCGCCGCGCC
GCGCCGCGCCGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTGTGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCGT
GCGGCGCGACGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTCGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTTGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ACCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAACGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGTCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGTGGGCAGTTGCTTTCG
GTTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCGCCGCGCC
GCGCCGCGCCGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTGTGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCGT
GCGGCGCGACGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTCGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTTGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ACCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAACGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGTCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGTGGGCAGTTGCTTTCG
GTTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA