Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00086  |
| T6SS type | Type i4b |
| Strain | Pseudomonas syringae pv. syringae B728a |
| Replicon (RefSeq) | chromosome (NC_007005) |
| Location | 5866174..5915475 |
| Function | effector translocation; adherence |
| Comment | - |
| References | [1] Sarris PF et al (2010) In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 11(6):795-804. [PudMed:21091602] [2] Boyer F et al (2009) Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 0.488888889. [PudMed:19284603] [3] Records AR et al (2010) Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 192(14):3584-96. [PudMed:20472799] [4] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | PAAR | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 2 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PSYR_RS25695 | 5863519..5863959 | + | 441 | WP_003401931.1 | hypothetical protein | ||
| PSYR_RS25700 | 5864093..5866027 | - | 1935 | WP_164920524.1 | RecQ family ATP-dependent DNA helicase | ||
| PSYR_RS25705 | 5866174..5866995 | + | 822 | WP_011269326.1 | hypothetical protein | ||
| PSYR_RS25710 | 5867626..5869797 | - | 2172 | WP_011269327.1 | M48 family metalloprotease | ||
| PSYR_RS25715 | 5870498..5871247 | + | 750 | WP_003401916.1 | hypothetical protein | ||
| T6CP000767 | TssB | PSYR_RS25720 (tssB) | 5871317..5871850 | + | 534 | WP_003318241.1 | type VI secretion system contractile sheath small subunit |
| T6CP000768 | TssC | PSYR_RS25725 (tssC) | 5871865..5873367 | + | 1503 | WP_003343625.1 | type VI secretion system contractile sheath large subunit |
| T6CP000769 | TssE | PSYR_RS25730 (tssE) | 5873395..5873877 | + | 483 | WP_003318239.1 | type VI secretion system baseplate subunit TssE |
| T6CP000770 | TssF | PSYR_RS25735 (tssF) | 5873877..5875709 | + | 1833 | WP_003343627.1 | type VI secretion system baseplate subunit TssF |
| T6CP000771 | TssG | PSYR_RS25740 (tssG) | 5875673..5876743 | + | 1071 | WP_011269329.1 | type VI secretion system baseplate subunit TssG |
| T6CP000772 | TssH | PSYR_RS25745 (tssH) | 5876740..5879343 | + | 2604 | WP_011269330.1 | type VI secretion system ATPase TssH |
| T6CP000773 | TssJ | PSYR_RS25750 (tssJ) | 5879369..5880124 | + | 756 | WP_011269331.1 | type VI secretion system lipoprotein TssJ |
| T6CP000774 | TssK | PSYR_RS25755 (tssK) | 5880121..5881464 | + | 1344 | WP_003343637.1 | type VI secretion system baseplate subunit TssK |
| T6CP000775 | TssL | PSYR_RS25760 (icmH) | 5881461..5882174 | + | 714 | WP_003318805.1 | type IVB secretion system protein IcmH/DotU |
| T6CP000776 | TssM | PSYR_RS25765 | 5882205..5886137 | + | 3933 | WP_011269332.1 | hypothetical protein |
| PSYR_RS25770 (tagF) | 5886169..5887092 | + | 924 | WP_003401903.1 | type VI secretion system-associated protein TagF | ||
| T6CP000777 | TssM | PSYR_RS25775 (tssM) | 5887089..5889581 | + | 2493 | WP_011269333.1 | type VI secretion system membrane subunit TssM |
| T6CP000778 | TssD | PSYR_RS25780 | 5889737..5890255 | + | 519 | WP_003318810.1 | type VI secretion system tube protein Hcp |
| T6CP000779 | TssA | PSYR_RS25785 (tssA) | 5890348..5891442 | + | 1095 | WP_003343647.1 | type VI secretion system protein TssA |
| PSYR_RS25790 | 5891620..5892045 | + | 426 | WP_011269334.1 | DUF2628 domain-containing protein | ||
| PSYR_RS25795 | 5892198..5892530 | + | 333 | WP_011269335.1 | hypothetical protein | ||
| T6CP011366 | PAAR | PSYR_RS25800 | 5892619..5892873 | + | 255 | WP_003401893.1 | PAAR domain-containing protein |
| PSYR_RS25805 | 5892924..5894978 | + | 2055 | WP_011269337.1 | phospholipase | ||
| PSYR_RS25810 | 5894981..5896006 | + | 1026 | WP_164920525.1 | sel1 repeat family protein | ||
| PSYR_RS25815 | 5896106..5897137 | + | 1032 | WP_011269339.1 | sel1 repeat family protein | ||
| PSYR_RS25820 | 5897201..5898283 | + | 1083 | WP_011269340.1 | hypothetical protein | ||
| T6CP000780 | TssI | PSYR_RS25825 | 5898317..5901193 | + | 2877 | WP_011269341.1 | type VI secretion system tip protein VgrG |
| PSYR_RS25830 | 5901334..5902053 | + | 720 | WP_011269342.1 | hypothetical protein | ||
| PSYR_RS25835 | 5902118..5902672 | - | 555 | WP_011269343.1 | bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase | ||
| PSYR_RS25840 | 5902749..5903081 | - | 333 | WP_080267554.1 | ADP-ribosylglycohydrolase family protein | ||
| PSYR_RS25845 | 5903248..5904363 | - | 1116 | WP_164920526.1 | ADP-ribosylglycohydrolase family protein | ||
| PSYR_RS25850 | 5904412..5904711 | - | 300 | WP_011269345.1 | hypothetical protein | ||
| PSYR_RS25855 | 5905568..5905873 | - | 306 | WP_024642655.1 | hypothetical protein | ||
| PSYR_RS25860 | 5906460..5907503 | + | 1044 | WP_024659562.1 | hypothetical protein | ||
| PSYR_RS25865 | 5907500..5908228 | + | 729 | WP_011269349.1 | hypothetical protein | ||
| T6CP000781 | TssI | PSYR_RS25870 (tssI) | 5908301..5910145 | + | 1845 | WP_011269350.1 | type VI secretion system tip protein VgrG |
| PSYR_RS25875 | 5910157..5910738 | + | 582 | WP_011269351.1 | DcrB-related protein | ||
| PSYR_RS25880 | 5910773..5911240 | + | 468 | WP_003318825.1 | DUF1795 domain-containing protein | ||
| T6CP011367 | PAAR | PSYR_RS25885 | 5911246..5915475 | + | 4230 | WP_011269352.1 | RHS domain-containing protein |
| PSYR_RS25890 | 5915482..5916090 | + | 609 | WP_003397046.1 | DUF1963 domain-containing protein | ||
| PSYR_RS25895 | 5916361..5917695 | + | 1335 | WP_164920577.1 | RHS repeat-associated core domain-containing protein | ||
| PSYR_RS25900 | 5917683..5918153 | + | 471 | WP_003318831.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0148 |
PSYR_RS19090 (relA) | protein | (p)ppGpp regulatory pathway | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 4380642..4382885 (-) | 499587809 | T6SS transcripts (↓) |
| REG0149 |
PSYR_RS01070 (spoT) | protein | (p)ppGpp regulatory pathway | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 223004..225109 (-) | 489499092 | T6SS transcripts (↓) |
| REG0150 |
Psyr_3698 (gacS) | protein | TCS-HK | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 4385550..4388303 (+) | 499587812 | T6SS transcription (↓) |
| REG0151 |
Psyr_4339 (ladS) | protein | QS regulatory pathway | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 5191591..5193585 (-) | 639463044 | T6SS transcription (↓) |
| REG0152 |
Psyr_4408 (retS) | protein | QS regulatory pathway | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 5270882..5273671 (+) | 489494774 | T6SS transcription (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00961 |
PSYR_RS25780 | - | - | Pseudomonas syringae pv. syringae B728a | chromosome (NC_007005) | 5889737..5890255 (+) | 66048189 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|