Detailed information of component protein


Summary

Component ID T6CP000776
Component protein type TssM
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (1311 a.a.); Nucleotide sequence (3933 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25765
Coordinate (Strand) 5882205..5886137 (+)
NCBI ID WP_011269332.1
RefSeq NC_007005
Uniprot ID Q4ZLI3_PSEU2, A0A2V4Q8W7_PSESY
KEGG ID psb:Psyr_4962
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 2.4e-54 182..442
IcmF-related PF06761.14 1.5e-46 498..918
IcmF_C PF06744.14 2.9e-18 1183..1282
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-11 MKTLLRLLKSF
Transmembrane helix 12-31 WFLVPILWLISLAVCWFFAP
Outside 32-34 RIS
Transmembrane helix 35-57 WLQGYTLEAMAIISAFYLLLIVL
Inside 58-440 RQYRRIRTEHNLENLVQIEVDRSLKSTGEFRDQQVLRDRLKHAIAMLRTDRSAGGGGSSALYDLPWYLVIGMSAAGKTSLLTRSGLSASIASSANDTESGTQHCDWYFSPEAVMIDTAGRYLRDDQSASEFAAFLRMLKKQRSKAAVNGLVLVVSLPELLAASSAERNEMGARLVTRIEEYTDCLDANPPIYLMLTKTDQLPGFNQAFDGMDLNERQQPLGMTFGLNEINTESLRTVLDRKLANLQAHVSRHVDAQIIALGADANSALLNFPNYFAELSAILEQFLQHFARSNVNGTQLLLRGLYFTSALQTDKQLTPVYEDELSESFVLRPAKPYGAVEEEEEEEEHRKISDRSYFITDTFRRVIFPDRDLTLYQSRHGRDR
Transmembrane helix 441-463 SIGPIVIAAALFAGLAFIGWQAL
Outside 464-1310 SFQKNREWLANISQQLRELEQSPDRAQRLASGQGLELLRNQLATIEKYRTKGVPLQLSGGLYRGDDIYFATQTAYLQQLRTQALEPITLKLQLQMREFNEFAKTMDPQYGFGAAPAGSDSKTGKARAQGRKLLDRGARNVAAGRPTSLSSIPRSTGDVTNRLTGAARNTASQTRSEAIAALRNPATANDAAELSATTGGLSLSEEMLGRLDERQVASIIESYNALKLYLILTQPTTHPETEFVNAALPVAWADISSEANPISDDVIEDNSPVYVQLLKNGDAPAMPRNEQLIDETRKSLKFFMISSSLVDREYLRLQLESSRQFPAIGLNDLVPMPGRQLLYGSEAVPAIFTRQGWEEFVKPELIKLVSGNLRNESDWVLDGEGGDSVVQKANFVREFMSRYKRDYTKVWYTMIDSVGVRRFSDMANATQQLSLLSDVRNSPVKLLLASVNDNTQWDVPAVREAQQTGVKRDDGFWGKVTGIFDNKEAAASVLVSPLPAVDDGSLAKRFEPVSRVFATQNAEGADSTIMDRYLAALRKLKVRMNNIQRSQDVGKSSKQLISETLEGQPSEVTNVRNYVETTVDTSQGGLSTSLQSLFSLPIQFAWETLRDPAGEQIAKAWAKQVAKPWEQVMAHRYPIAADSRNEASVKDLQRFVDPESGLLPNFKRNEIGNLSDGEGLGMGSGAKAAPLVNPKMVSNIDKASSLGEVIASLSDRDNGFEIMLEPSAYFTDIIFTLDGQEQHYRNGKTSWSRFSWPGTTTAPGARLDVVTLSGERITVFDYTGRWGLLRMNDSARVADLDGIQQRFSWNTAKGPVSLVVRNYGGVKLTDLANVKALSALNATDGRTK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9655 - -


  Protein sequence: 1311 a.a.    

>T6CP000776 NC_007005:5882205-5886137 [Pseudomonas syringae pv. syringae B728a] [TssM]
MKTLLRLLKSFWFLVPILWLISLAVCWFFAPRISWLQGYTLEAMAIISAFYLLLIVLRQYRRIRTEHNLENLVQIEVDRS
LKSTGEFRDQQVLRDRLKHAIAMLRTDRSAGGGGSSALYDLPWYLVIGMSAAGKTSLLTRSGLSASIASSANDTESGTQH
CDWYFSPEAVMIDTAGRYLRDDQSASEFAAFLRMLKKQRSKAAVNGLVLVVSLPELLAASSAERNEMGARLVTRIEEYTD
CLDANPPIYLMLTKTDQLPGFNQAFDGMDLNERQQPLGMTFGLNEINTESLRTVLDRKLANLQAHVSRHVDAQIIALGAD
ANSALLNFPNYFAELSAILEQFLQHFARSNVNGTQLLLRGLYFTSALQTDKQLTPVYEDELSESFVLRPAKPYGAVEEEE
EEEEHRKISDRSYFITDTFRRVIFPDRDLTLYQSRHGRDRSIGPIVIAAALFAGLAFIGWQALSFQKNREWLANISQQLR
ELEQSPDRAQRLASGQGLELLRNQLATIEKYRTKGVPLQLSGGLYRGDDIYFATQTAYLQQLRTQALEPITLKLQLQMRE
FNEFAKTMDPQYGFGAAPAGSDSKTGKARAQGRKLLDRGARNVAAGRPTSLSSIPRSTGDVTNRLTGAARNTASQTRSEA
IAALRNPATANDAAELSATTGGLSLSEEMLGRLDERQVASIIESYNALKLYLILTQPTTHPETEFVNAALPVAWADISSE
ANPISDDVIEDNSPVYVQLLKNGDAPAMPRNEQLIDETRKSLKFFMISSSLVDREYLRLQLESSRQFPAIGLNDLVPMPG
RQLLYGSEAVPAIFTRQGWEEFVKPELIKLVSGNLRNESDWVLDGEGGDSVVQKANFVREFMSRYKRDYTKVWYTMIDSV
GVRRFSDMANATQQLSLLSDVRNSPVKLLLASVNDNTQWDVPAVREAQQTGVKRDDGFWGKVTGIFDNKEAAASVLVSPL
PAVDDGSLAKRFEPVSRVFATQNAEGADSTIMDRYLAALRKLKVRMNNIQRSQDVGKSSKQLISETLEGQPSEVTNVRNY
VETTVDTSQGGLSTSLQSLFSLPIQFAWETLRDPAGEQIAKAWAKQVAKPWEQVMAHRYPIAADSRNEASVKDLQRFVDP
ESGLLPNFKRNEIGNLSDGEGLGMGSGAKAAPLVNPKMVSNIDKASSLGEVIASLSDRDNGFEIMLEPSAYFTDIIFTLD
GQEQHYRNGKTSWSRFSWPGTTTAPGARLDVVTLSGERITVFDYTGRWGLLRMNDSARVADLDGIQQRFSWNTAKGPVSL
VVRNYGGVKLTDLANVKALSALNATDGRTK*

  Nucleotide sequence: 3933 bp    

>T6CP000776 NC_007005:5882205-5886137 [Pseudomonas syringae pv. syringae B728a] [TssM]
ATGAAGACATTACTGCGCCTGTTAAAAAGTTTCTGGTTCCTGGTCCCGATACTGTGGCTGATCAGCCTGGCTGTCTGCTG
GTTTTTCGCCCCAAGGATCAGTTGGCTGCAAGGCTACACGCTCGAAGCGATGGCGATCATCAGCGCCTTTTACCTGCTGC
TGATCGTACTGCGCCAATACCGCCGGATTCGCACCGAACACAACCTCGAGAATCTGGTTCAGATCGAAGTTGACCGCTCG
CTGAAGTCCACTGGCGAGTTCCGTGATCAGCAAGTGCTGCGCGACCGCCTCAAGCATGCCATTGCCATGCTGCGCACCGA
TCGTTCGGCAGGCGGCGGCGGCTCCTCGGCGCTCTACGATCTGCCCTGGTATCTGGTCATCGGCATGTCCGCAGCGGGCA
AGACCTCGCTGCTGACCCGCTCCGGCCTGTCGGCCAGCATTGCCAGCAGCGCCAATGACACGGAAAGCGGCACCCAGCAC
TGCGACTGGTACTTCAGCCCTGAAGCCGTGATGATCGACACCGCTGGCCGCTACCTTCGCGATGATCAGTCCGCCAGCGA
ATTCGCGGCGTTCCTGCGCATGCTCAAAAAGCAGCGCAGCAAGGCCGCCGTCAATGGTCTGGTGCTGGTGGTCAGCCTGC
CGGAACTGTTGGCCGCTTCTTCCGCCGAACGTAACGAAATGGGTGCGCGCCTGGTCACGCGCATCGAGGAATACACCGAT
TGCCTGGACGCCAATCCACCGATCTACCTGATGCTGACCAAGACTGACCAACTGCCCGGCTTCAACCAGGCCTTCGACGG
CATGGACCTGAACGAGCGCCAGCAGCCGCTGGGCATGACGTTCGGGCTGAACGAGATCAACACCGAAAGCCTGCGCACGG
TGCTCGATCGCAAGCTGGCGAACCTGCAGGCGCATGTCAGTCGCCATGTCGATGCACAGATCATCGCGCTGGGTGCCGAT
GCCAACAGCGCCCTGCTGAACTTCCCCAACTACTTTGCCGAGCTGTCGGCGATACTCGAGCAGTTCCTGCAGCATTTCGC
GCGCAGTAACGTCAACGGTACTCAGTTGTTGCTGCGCGGCCTGTATTTCACCAGCGCCCTGCAAACCGACAAGCAACTGA
CACCGGTGTATGAAGACGAGCTTTCAGAGTCGTTCGTCCTGCGTCCGGCGAAGCCATACGGTGCAGTGGAGGAAGAGGAA
GAGGAGGAAGAACACCGGAAAATCAGTGATCGCAGTTACTTCATCACCGACACCTTCCGCCGCGTCATTTTCCCTGACCG
TGACCTGACCCTGTACCAGTCACGGCATGGCCGCGACCGCTCTATTGGCCCGATCGTCATCGCTGCGGCGCTGTTCGCCG
GGCTGGCCTTCATCGGCTGGCAAGCCCTGTCGTTCCAGAAAAACCGCGAGTGGCTGGCCAACATCAGCCAGCAGTTGCGC
GAACTGGAGCAGTCCCCGGACCGGGCACAACGCCTTGCCTCGGGCCAAGGCCTGGAGCTGCTGCGCAATCAGCTGGCAAC
CATCGAGAAATACCGCACCAAGGGCGTACCGCTGCAACTCAGCGGCGGCCTGTACCGTGGCGACGATATCTACTTCGCCA
CCCAGACCGCCTACCTGCAGCAACTGCGTACCCAGGCGCTTGAGCCGATCACGCTGAAACTGCAATTGCAGATGCGTGAA
TTCAACGAGTTCGCCAAAACCATGGACCCGCAGTATGGCTTCGGGGCCGCTCCCGCCGGTTCCGACAGCAAAACCGGCAA
GGCGCGTGCGCAAGGCCGGAAACTGCTGGACCGTGGCGCTCGCAACGTCGCGGCAGGCCGTCCGACCAGCCTGAGCAGCA
TTCCGCGCAGCACCGGCGACGTGACCAATCGCCTGACCGGCGCCGCGAGAAACACGGCCAGCCAGACACGCAGCGAGGCC
ATCGCCGCACTGCGTAACCCGGCCACCGCAAACGATGCGGCCGAGTTGTCGGCCACCACGGGCGGTCTGTCGCTGTCGGA
AGAGATGCTCGGACGACTCGATGAACGGCAGGTGGCATCCATCATCGAGTCATACAACGCTCTCAAGCTTTATCTGATTC
TGACTCAGCCGACCACGCACCCGGAAACCGAGTTCGTCAACGCGGCGTTGCCGGTGGCCTGGGCGGATATTTCCAGCGAG
GCCAATCCGATCAGCGACGACGTCATCGAGGACAACTCGCCGGTCTATGTACAGTTGCTCAAGAACGGCGACGCGCCGGC
CATGCCGCGCAATGAGCAGTTGATCGACGAGACCCGCAAGAGCCTGAAGTTTTTCATGATCTCCAGCTCGCTGGTGGACC
GTGAATACCTGCGCCTGCAACTGGAATCCAGCCGTCAGTTCCCCGCTATCGGCCTGAATGATCTGGTGCCGATGCCGGGT
CGTCAGTTGCTGTACGGTTCGGAAGCCGTGCCTGCGATTTTCACCCGTCAGGGCTGGGAAGAGTTCGTGAAGCCCGAGCT
GATCAAGCTGGTGTCCGGCAATCTGCGCAACGAATCGGACTGGGTGCTCGACGGTGAAGGCGGCGACAGCGTGGTGCAGA
AAGCCAACTTCGTCCGTGAGTTCATGTCCCGTTACAAGCGTGACTACACGAAAGTCTGGTACACCATGATCGACAGCGTG
GGCGTACGTCGCTTCAGCGACATGGCCAACGCCACGCAGCAGTTGTCATTGCTCAGTGACGTGCGCAATTCGCCGGTCAA
GCTGCTGTTGGCCTCAGTCAACGACAACACGCAATGGGACGTCCCTGCAGTTCGCGAGGCGCAACAGACCGGGGTCAAGC
GCGACGATGGCTTCTGGGGCAAGGTCACCGGCATCTTCGACAACAAGGAAGCGGCTGCCAGCGTACTGGTCTCGCCGCTG
CCTGCAGTGGATGACGGCAGTCTGGCCAAGCGCTTCGAGCCTGTATCGCGGGTTTTCGCCACACAGAACGCCGAAGGCGC
CGACAGCACCATCATGGATCGCTACCTGGCAGCGCTGCGTAAGCTCAAAGTGCGGATGAACAACATCCAGCGCTCGCAGG
ATGTCGGCAAGAGCAGCAAGCAGTTGATCAGCGAAACCCTCGAAGGGCAGCCGAGCGAAGTCACCAACGTGCGTAACTAC
GTTGAAACCACGGTCGATACCAGTCAGGGCGGGCTTTCCACTTCGCTGCAAAGCCTGTTCAGCCTGCCGATTCAGTTCGC
CTGGGAAACGCTGCGTGATCCGGCTGGCGAACAGATCGCCAAAGCCTGGGCCAAACAGGTCGCCAAGCCCTGGGAACAGG
TCATGGCTCACCGGTACCCGATTGCTGCGGACAGCCGCAACGAGGCCTCGGTGAAAGACCTGCAGCGTTTCGTCGACCCG
GAGTCGGGCCTGCTGCCCAACTTCAAACGCAATGAAATCGGCAACCTGTCCGATGGCGAAGGGCTCGGCATGGGCAGTGG
TGCCAAGGCGGCGCCGCTGGTCAACCCGAAAATGGTCAGCAACATCGACAAGGCCAGTTCGCTGGGCGAAGTCATCGCCA
GCCTCTCGGACCGCGACAACGGCTTCGAGATCATGCTTGAGCCGTCGGCCTACTTCACCGATATCATCTTCACGCTGGAT
GGCCAGGAGCAGCACTACCGCAACGGCAAGACCAGCTGGAGCCGCTTCTCGTGGCCTGGCACTACCACAGCGCCAGGCGC
ACGCCTGGATGTGGTCACCCTGTCAGGCGAACGCATTACCGTGTTCGACTACACCGGCCGCTGGGGCCTGCTGCGCATGA
ACGACAGCGCACGCGTCGCTGACCTCGACGGCATCCAGCAGCGATTCAGCTGGAACACGGCCAAAGGGCCGGTCAGTCTG
GTGGTACGCAACTATGGCGGCGTGAAGCTGACCGACCTGGCCAACGTCAAGGCGCTGAGCGCGCTTAACGCAACCGATGG
GCGGACCAAGTGA