Detailed information of regulator


Summary

Regulator ID REG0148 experimental
Regulator type protein
Regulatory pathway (from literatures) (p)ppGpp regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Purine metabolism (map00230)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (748 a.a.); Nucleotide sequence (2244 bp)
Description 28 putative T6SS-associated genes of PstDC3000 and 25 T6SS-related genes of PssB728a were down-regulated in the (p)ppGpp0PstDC3000 and (p)ppGpp0PssB728a mutants, respectively.
Reference
External database links

Locus tag (Gene) PSYR_RS19090 (relA)
Coordinates (Strand) 4380642..4382885 (-)
NCBI ID 499587809
RefSeq NC_007005
Uniprot ID Q4ZQ45_PSEU2, A0A2V3QJ80_9PSED, A0A108ULB7_PSESY
KEGG ID psb:Psyr_3695
KEGG pathway (determined by BlastKOALA) map01100; map00230
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
T6SS transcripts↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
HD_4PF13328.83.5e-4440..202
RelA_SpoTPF04607.199.9e-38262..370
TGSPF02824.237.9e-22416..475
ACT_4PF13291.88.2e-17667..743
DUF5913PF19296.12.7e-08494..637
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-747MVQVRANQPINTDGSINLDAWLDHIVSVDLVLDREVMKKACEFAREAEQTDKAHKNHWAEGTSSFQTGLEIAEILADLKLDQDSLVAAVIYRGVREGLIALPDVEQRFGATVAKLIDGVLRMAAISASLSPRQSLVLGSQAQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKNADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFISDVMSQLDNELQATGVTADISGRAKHIYSIWRKMQRKGLAFSQIYDVRALRVLVPEMRDCYTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAELGVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDIGGLADQLRVDIEPDRVYVFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGTPSRDWLNSNLGYITTSRARAKIVHWFKLQARDQNVAAGRTLIERELARLALPQVDFDKLADKANHKTADDMFAALGAGDLRLAQLVNLAQQQVEPDRVNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLGGREPERIIQVSWGPVPVLTYPVDIIIRAYDRSGLLRDVTQVLLNERINVLAVNTRSNKEDNTALMSLTIEIPGLDALGRLLGRISQLPNIIETRRNRTP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9966--


  Protein sequence of regulator: 748 a.a.    .

>REG0148 NC_007005:c4382885-4380642 [Pseudomonas syringae pv. syringae B728a]
MVQVRANQPINTDGSINLDAWLDHIVSVDLVLDREVMKKACEFAREAEQTDKAHKNHWAEGTSSFQTGLEIAEILADLKL
DQDSLVAAVIYRGVREGLIALPDVEQRFGATVAKLIDGVLRMAAISASLSPRQSLVLGSQAQVENLRKMLVAMVDDVRVA
LIKLAERTCAIRAVKNADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
SDVMSQLDNELQATGVTADISGRAKHIYSIWRKMQRKGLAFSQIYDVRALRVLVPEMRDCYTALGIVHTLWRHIPKEFDD
YIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAELGVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDI
GGLADQLRVDIEPDRVYVFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNSNLGYITTSRARAKIVHWFKLQARDQNVAAGRTLIERELARLALPQVDFDKLADKANHKTADDMFAALGAGDL
RLAQLVNLAQQQVEPDRVNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQD
CASVLQLGGREPERIIQVSWGPVPVLTYPVDIIIRAYDRSGLLRDVTQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP*

  Nucleotide sequence of regulator: 2244 bp    .

>REG0148 NC_007005:c4382885-4380642 [Pseudomonas syringae pv. syringae B728a]
ATGGTACAGGTGAGAGCGAACCAGCCGATAAACACAGACGGCAGTATCAATCTCGACGCGTGGCTCGATCATATTGTCAG
CGTCGACCTGGTCCTGGACCGGGAAGTCATGAAAAAGGCCTGTGAATTTGCCCGGGAAGCCGAGCAGACCGACAAGGCTC
ACAAGAATCATTGGGCAGAGGGCACCTCCAGTTTCCAGACCGGTCTGGAAATCGCGGAGATCCTGGCTGACCTCAAGCTG
GATCAGGACTCGCTGGTCGCTGCGGTCATTTACCGTGGCGTGCGTGAAGGTCTCATTGCGCTGCCGGACGTCGAGCAGCG
CTTTGGCGCAACGGTCGCCAAACTCATCGACGGCGTGCTGCGCATGGCCGCGATCAGCGCCAGCCTCAGCCCTCGTCAGT
CATTGGTGCTCGGCAGTCAGGCGCAGGTGGAAAACCTGCGCAAGATGCTGGTGGCGATGGTCGACGACGTTCGCGTTGCG
CTGATCAAGCTGGCCGAACGTACCTGCGCGATTCGTGCGGTGAAGAATGCCGACGACGAAAAACGCAACCGTGTGGCTCG
CGAAGTCTTCGACATCTATGCGCCACTGGCCCACCGCCTGGGTATCGGGCATATCAAGTGGGAGCTGGAGGACCTGTCCT
TCCGCTACCTGGAGCCCGATCAATACAAGCAGATCGCCAAGCTGCTGCATGAACGCAGGCTGGACCGCGAGCGCTTCATC
AGCGACGTCATGTCTCAACTCGATAACGAGCTGCAGGCCACCGGCGTGACCGCTGACATCAGCGGTCGTGCCAAGCATAT
CTACTCCATCTGGCGCAAAATGCAGCGCAAGGGCCTGGCGTTCAGCCAGATCTATGACGTGCGTGCGTTGCGTGTGCTGG
TCCCGGAAATGCGCGACTGCTACACCGCGCTGGGTATCGTGCACACCTTGTGGCGGCATATTCCCAAAGAGTTCGACGAT
TACATCGCCAACCCCAAGGAAAACGGCTATCGCTCGCTGCACACCGCCGTGATCGGGCCGGAAGGCAAGGTGCTGGAAGT
GCAGATCCGCACCCACGCCATGCATGAAGAGGCCGAGCTGGGCGTTTGTGCGCACTGGCGCTATAAAGGCACCGACGTCA
AATCCGGCTCCAATCATTACGAAGAGAAGATTTCCTGGCTGCGTCAGGTGCTCGAATGGCATGAGGAGCTGGGCGACATC
GGCGGTCTGGCCGATCAGTTGCGGGTTGATATCGAGCCCGACCGCGTCTACGTCTTCACCCCGGACGGCCACGCCATCGA
CCTGCCCAAGGGCGCGACACCGCTGGACTTCGCCTATCGGGTGCACACCGAAATCGGCCATAACTGCCGGGGTGCGAAGA
TCAATGGCCGCATCGTACCGCTCAACTACAGCCTGCAGACCGGCGAGCAGGTCGAGATCATCACCAGCAAGCACGGTACG
CCGAGCCGCGACTGGCTGAACTCGAACCTGGGCTACATCACCACCTCGCGCGCGCGGGCCAAGATCGTTCACTGGTTCAA
GTTGCAGGCTCGCGACCAGAACGTCGCCGCCGGCAGAACCCTGATCGAACGCGAACTGGCGCGCCTGGCGTTACCGCAGG
TCGATTTCGACAAGCTGGCGGACAAGGCCAATCACAAGACCGCCGACGACATGTTTGCGGCATTGGGCGCGGGCGATCTG
CGTCTGGCGCAACTGGTCAATCTGGCTCAGCAGCAGGTTGAGCCTGATCGCGTCAATGAACAGCTGGAGCTGATCCCGCG
CAAGGCCACCGGCTACAAGCCCGGCAAGCGTGGCGATATTCAGATTCAGGGCGTCGGCAACCTGATGACGCAGATGGCCG
GCTGCTGCCAGCCGCTGCCGGGCGATGCGATCGTCGGTTATATCACTCAGGGGCGTGGCGTCAGCATTCACCGTCAGGAT
TGCGCTTCGGTGCTGCAATTGGGCGGTCGCGAGCCCGAGCGGATCATTCAGGTCAGTTGGGGGCCGGTGCCGGTACTCAC
CTATCCGGTGGACATCATCATCCGTGCCTACGACCGTTCCGGCTTGCTCCGGGACGTCACCCAGGTGCTGCTCAACGAGC
GGATCAACGTGCTGGCGGTCAATACCCGTTCGAACAAGGAAGACAACACAGCGCTCATGTCTCTGACCATCGAGATTCCG
GGTCTGGACGCGCTGGGGCGGTTGCTGGGGCGCATTTCCCAGCTGCCGAACATCATTGAAACGCGCAGGAACAGGACGCC
CTAG