Detailed information of component protein


Summary

Component ID T6CP000780
Component protein type TssI
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (959 a.a.); Nucleotide sequence (2877 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25825
Coordinate (Strand) 5898317..5901193 (+)
NCBI ID WP_011269341.1
RefSeq NC_007005
Uniprot ID Q4ZLH1_PSEU2, A0A2V4PQS3_PSESY
KEGG ID psb:Psyr_4974
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 4.5e-78 42..349
DUF2345 PF10106.11 4.4e-52 675..823
T6SS_Vgr PF13296.8 6.6e-40 513..620
Phage_base_V PF04717.14 8e-12 427..494
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-958 MNSILDAAMNAFSGPLSQAGRLLELNTPQGQELFALRAHAVERLSHVARYTVDVINQTTDFDPEKLIGQAVQLSIRLDDGSLSPRHGWVESVRYLGSDGGLEDWQLVFAPWFSLLEYRIDCRIWQEMSLPDILAQVFQHYSQARANYRFDLQRDYPLLSYVTQFNETDANFVQRWCEQEGLFWYVEHKVDSHCIVFTDNIDSLPTLNPASLRFHTQQAADLKDSITQWSPGARLLNGLVQWRSSEYRSHAQPQITHSMALPAASAPPDLERYQYRGQYAWQNLGRGEWLARVQIEQDESAARRIQGQGGARQMQPGHGFELTQHPLYELKPLQDRQFLIVAVEFFVQSNLPIGKTRSSPPGSLKTQLDALQTQASLPAGDEGFYLNRFETQRLDIPYRSAFTHQKPAHPGPQTAVVVTPGGHEVFTDNLNRVCVRFHWDRLVKDGELGSCWLRMMQSSSGQDWGSVNVPRAGEEVVVTFLDNDIDRPLIMGQVYGGHKPAWHSSGLMSGYKSQEIQGSGYNQWVMDDSTGQVRTQIHSSHGHSQLNLGYLIDQQGNSRGALRGAGFELRTDAYGALRAQQGLYLSTWPRNKAQGGQIDTQEAQQHLSNAQARVKALSEIAEQHNALPMPGGIESLDQLNADTQANYGSGGESPVSSSPQQRNGGDTKAAIMSGGRGQTSGYKKPLLVASSPADIATVTPQNTHIHSGKQLTLSTGEDTNLASGKSLLVSVAQSISLFAQAAGAKLFAAKGKVEIQAQNDSIELTAKESVRITSTARTIEIAAQEEILLTSGAAYIRIKGGNIEIHAPGTIDVKGVKKTFDGPAQLNRDNPAWPTDSIKQKLTAYAGLSSAAGYQAWAGQPYTIFADGAEVKKGVMDASGQIAIDHHATTSQYRIDLSNGVSYDLPVAADYKGDPKNAELANRGLHRHESASHPDVKPSDNRSLFRNRYDQLSNPESEA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9480 - -


  Protein sequence: 959 a.a.    

>T6CP000780 NC_007005:5898317-5901193 [Pseudomonas syringae pv. syringae B728a] [TssI]
MNSILDAAMNAFSGPLSQAGRLLELNTPQGQELFALRAHAVERLSHVARYTVDVINQTTDFDPEKLIGQAVQLSIRLDDG
SLSPRHGWVESVRYLGSDGGLEDWQLVFAPWFSLLEYRIDCRIWQEMSLPDILAQVFQHYSQARANYRFDLQRDYPLLSY
VTQFNETDANFVQRWCEQEGLFWYVEHKVDSHCIVFTDNIDSLPTLNPASLRFHTQQAADLKDSITQWSPGARLLNGLVQ
WRSSEYRSHAQPQITHSMALPAASAPPDLERYQYRGQYAWQNLGRGEWLARVQIEQDESAARRIQGQGGARQMQPGHGFE
LTQHPLYELKPLQDRQFLIVAVEFFVQSNLPIGKTRSSPPGSLKTQLDALQTQASLPAGDEGFYLNRFETQRLDIPYRSA
FTHQKPAHPGPQTAVVVTPGGHEVFTDNLNRVCVRFHWDRLVKDGELGSCWLRMMQSSSGQDWGSVNVPRAGEEVVVTFL
DNDIDRPLIMGQVYGGHKPAWHSSGLMSGYKSQEIQGSGYNQWVMDDSTGQVRTQIHSSHGHSQLNLGYLIDQQGNSRGA
LRGAGFELRTDAYGALRAQQGLYLSTWPRNKAQGGQIDTQEAQQHLSNAQARVKALSEIAEQHNALPMPGGIESLDQLNA
DTQANYGSGGESPVSSSPQQRNGGDTKAAIMSGGRGQTSGYKKPLLVASSPADIATVTPQNTHIHSGKQLTLSTGEDTNL
ASGKSLLVSVAQSISLFAQAAGAKLFAAKGKVEIQAQNDSIELTAKESVRITSTARTIEIAAQEEILLTSGAAYIRIKGG
NIEIHAPGTIDVKGVKKTFDGPAQLNRDNPAWPTDSIKQKLTAYAGLSSAAGYQAWAGQPYTIFADGAEVKKGVMDASGQ
IAIDHHATTSQYRIDLSNGVSYDLPVAADYKGDPKNAELANRGLHRHESASHPDVKPSDNRSLFRNRYDQLSNPESEA*

  Nucleotide sequence: 2877 bp    

>T6CP000780 NC_007005:5898317-5901193 [Pseudomonas syringae pv. syringae B728a] [TssI]
ATGAATTCGATACTCGACGCTGCCATGAACGCCTTCAGCGGACCGCTCAGTCAGGCCGGTCGCCTGCTGGAATTGAACAC
GCCGCAAGGTCAGGAGCTGTTTGCTCTGCGCGCGCATGCCGTGGAACGTCTGAGCCACGTGGCGCGCTACACGGTCGACG
TGATCAATCAGACCACCGACTTCGACCCGGAGAAGCTGATTGGTCAGGCAGTGCAACTGAGCATTCGCCTCGACGACGGC
AGCCTGAGCCCTCGGCATGGCTGGGTCGAGAGTGTGCGTTATCTCGGCAGCGATGGCGGGCTCGAGGATTGGCAGCTGGT
CTTTGCGCCGTGGTTCAGCCTGCTGGAATACCGTATTGATTGCCGAATCTGGCAAGAAATGAGCTTGCCGGACATCCTTG
CTCAGGTGTTTCAGCACTACAGCCAGGCCCGCGCCAACTACCGCTTCGACCTGCAACGCGACTACCCGCTGCTGTCATAC
GTGACGCAGTTCAACGAGACCGACGCCAACTTCGTGCAGCGCTGGTGCGAGCAGGAAGGCCTTTTCTGGTACGTGGAGCA
CAAGGTCGACAGTCATTGCATCGTGTTTACCGACAACATTGACTCTCTGCCCACACTGAACCCCGCAAGCCTGCGTTTCC
ATACCCAGCAGGCCGCCGACCTGAAAGACAGCATCACCCAATGGAGCCCCGGCGCCCGCCTGCTCAATGGCCTGGTGCAG
TGGCGCAGCAGCGAATACCGCAGCCATGCGCAGCCACAGATCACTCACTCCATGGCACTGCCTGCCGCATCCGCGCCTCC
TGACCTCGAGCGTTACCAATACCGTGGCCAGTATGCATGGCAGAACCTCGGCCGCGGCGAATGGCTCGCGCGCGTGCAGA
TCGAACAGGATGAGTCGGCGGCCCGTCGCATTCAGGGTCAGGGCGGGGCTCGTCAGATGCAGCCCGGTCATGGCTTTGAG
CTGACCCAACATCCGCTTTACGAGCTCAAGCCGCTCCAGGACCGTCAGTTCCTGATCGTCGCCGTGGAGTTCTTTGTGCA
GAGCAACCTGCCAATCGGCAAGACGCGATCAAGCCCACCCGGGAGCCTGAAAACACAGTTGGATGCACTGCAGACGCAAG
CTTCGCTACCGGCGGGCGATGAAGGCTTCTACCTGAATCGCTTTGAAACACAGCGTCTGGATATTCCCTATCGCAGCGCG
TTTACCCATCAAAAACCGGCGCACCCCGGCCCGCAGACAGCAGTCGTCGTGACACCCGGCGGGCATGAGGTTTTCACCGA
TAACCTGAACCGGGTTTGCGTTCGCTTTCACTGGGATCGTCTGGTGAAGGATGGAGAGCTTGGTTCGTGCTGGCTGCGCA
TGATGCAGTCGAGCAGCGGTCAGGACTGGGGCAGCGTCAATGTGCCGCGCGCTGGCGAAGAGGTAGTCGTCACCTTTCTT
GATAACGATATCGACCGTCCGCTGATCATGGGACAGGTCTACGGCGGACATAAGCCTGCGTGGCATTCCAGCGGCCTGAT
GAGTGGCTATAAAAGTCAGGAGATTCAGGGGAGCGGCTATAACCAGTGGGTGATGGACGATTCCACCGGCCAGGTCAGAA
CCCAGATCCACAGTAGCCATGGGCATAGTCAGCTGAACCTCGGTTACCTGATCGACCAACAGGGCAATTCACGAGGCGCA
CTGCGCGGTGCCGGTTTCGAGCTGCGGACTGACGCCTACGGTGCCTTGCGTGCTCAGCAAGGTCTGTACTTGAGCACTTG
GCCGCGCAACAAGGCGCAGGGCGGGCAGATTGATACCCAGGAAGCTCAACAGCATCTGAGCAACGCTCAGGCACGAGTGA
AGGCATTGTCGGAAATTGCCGAGCAGCACAATGCGCTGCCCATGCCGGGCGGTATTGAAAGTCTCGACCAGCTCAACGCC
GATACCCAGGCCAATTACGGCAGCGGCGGCGAAAGCCCGGTCAGCAGCAGTCCGCAGCAACGTAACGGCGGTGATACCAA
GGCGGCGATCATGAGCGGCGGTCGTGGGCAGACGTCAGGCTATAAAAAGCCCTTGCTGGTCGCATCCTCGCCCGCCGACA
TCGCGACCGTCACCCCGCAAAATACTCACATCCACAGCGGTAAACAGTTAACGCTTTCAACCGGTGAAGACACCAATCTG
GCCAGCGGCAAGTCACTATTGGTGAGTGTCGCGCAAAGCATCAGTCTGTTCGCCCAGGCGGCAGGCGCCAAACTGTTTGC
AGCAAAGGGCAAAGTTGAAATACAGGCGCAAAACGACAGCATTGAACTGACCGCGAAGGAAAGCGTGCGCATCACCTCTA
CGGCTCGCACTATCGAGATCGCAGCGCAGGAAGAAATCCTGCTGACCAGCGGCGCAGCGTACATCCGCATCAAGGGTGGC
AATATCGAGATTCATGCTCCCGGAACCATCGACGTAAAAGGCGTGAAAAAGACTTTCGACGGCCCTGCGCAGCTCAATCG
GGATAATCCGGCGTGGCCGACCGACTCGATCAAACAAAAGCTTACAGCCTACGCCGGGCTTAGCAGTGCGGCGGGCTATC
AGGCATGGGCAGGTCAGCCGTATACCATCTTTGCAGACGGGGCCGAGGTCAAAAAAGGAGTGATGGACGCCAGCGGACAG
ATCGCCATCGACCATCACGCTACAACCAGCCAGTACCGTATTGATCTGAGTAACGGCGTCAGCTACGACCTGCCAGTAGC
GGCCGATTACAAAGGTGATCCCAAAAATGCGGAACTGGCCAACCGCGGGCTTCACCGCCACGAAAGCGCTTCGCACCCAG
ACGTCAAACCGAGCGACAATCGCAGTCTGTTCCGTAACAGGTATGACCAGTTGAGCAATCCAGAGTCGGAGGCGTGA