Detailed information of component protein


Summary

Component ID T6CP000773
Component protein type TssJ
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (252 a.a.); Nucleotide sequence (756 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25750
Coordinate (Strand) 5879369..5880124 (+)
NCBI ID WP_011269331.1
RefSeq NC_007005
Uniprot ID Q4ZLI6_PSEU2, A0A2V4PQ74_PSESY
KEGG ID psb:Psyr_4959
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 2.5e-28 56..177
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-251 MNKRLQGNCSSKPALARCLAVLAMMMALSACGVTDRVAKRMDDTWAGDMMFGDNEKVILTTDGGNQLNPDEYGKPLSVVVRVYQLSSLERFESINADTLWEDPQKALGNTLVESRELILLPGMGQTDQWPLNPNASYVGIAAFFRTDVDSRWKVAFDANTLRKDGIWFSSDGVRVLVDNNYILAVRGVDVLNKLKTEEQFNKSNSIPEQPASKSMTERAQDAAVQQVQDSAGSAAKKAADSQFNSLLEGAK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9942 pos: 30-31. LSA-CG. MNKRLQGNCSSKPALARCLAVLAMMMALSA


  Protein sequence: 252 a.a.    

>T6CP000773 NC_007005:5879369-5880124 [Pseudomonas syringae pv. syringae B728a] [TssJ]
MNKRLQGNCSSKPALARCLAVLAMMMALSACGVTDRVAKRMDDTWAGDMMFGDNEKVILTTDGGNQLNPDEYGKPLSVVV
RVYQLSSLERFESINADTLWEDPQKALGNTLVESRELILLPGMGQTDQWPLNPNASYVGIAAFFRTDVDSRWKVAFDANT
LRKDGIWFSSDGVRVLVDNNYILAVRGVDVLNKLKTEEQFNKSNSIPEQPASKSMTERAQDAAVQQVQDSAGSAAKKAAD
SQFNSLLEGAK*

  Nucleotide sequence: 756 bp    

>T6CP000773 NC_007005:5879369-5880124 [Pseudomonas syringae pv. syringae B728a] [TssJ]
ATGAATAAAAGACTTCAAGGCAATTGTTCCTCGAAGCCAGCCTTGGCAAGGTGCCTGGCTGTTCTGGCAATGATGATGGC
TCTGTCCGCCTGCGGCGTGACAGACCGTGTCGCCAAGCGCATGGATGACACCTGGGCTGGCGACATGATGTTCGGTGACA
ATGAAAAGGTCATCCTGACGACTGACGGCGGCAACCAGCTCAACCCCGACGAGTACGGCAAACCGCTCTCGGTGGTGGTG
CGCGTTTATCAACTGTCCTCTCTGGAGCGCTTCGAGTCGATCAACGCCGACACTCTCTGGGAGGATCCGCAAAAGGCTCT
GGGCAACACGCTGGTCGAGAGCCGGGAGCTGATTCTGTTGCCTGGCATGGGCCAGACCGATCAGTGGCCGCTGAACCCGA
ATGCCTCGTATGTGGGCATCGCGGCGTTCTTCCGCACCGACGTCGACAGCCGCTGGAAGGTCGCGTTCGACGCCAACACG
CTGCGCAAGGACGGTATCTGGTTCTCGTCCGACGGCGTGCGTGTGCTGGTGGATAACAACTACATCCTCGCGGTGCGTGG
TGTGGACGTGCTGAACAAACTCAAGACTGAAGAGCAGTTCAACAAATCCAATTCGATTCCCGAACAACCCGCCAGTAAAA
GTATGACCGAGCGCGCGCAGGACGCAGCTGTCCAGCAGGTTCAGGACTCGGCCGGCAGTGCTGCCAAAAAGGCAGCTGAC
TCACAATTCAATTCTCTGTTGGAAGGCGCCAAATGA