Detailed information of component protein


Summary

Component ID T6CP011367
Component protein type PAAR
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (1410 a.a.); Nucleotide sequence (4230 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25885
Coordinate (Strand) 5911246..5915475 (+)
NCBI ID WP_011269352.1
RefSeq NC_007005
Uniprot ID Q4ZLF9_PSEU2, A0A2V4PP94_PSESY
KEGG ID psb:Psyr_4986
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF6531 PF20148.1 4.1e-21 232..305
RHS PF03527.16 6.6e-19 1173..1206
PAAR_motif PF05488.15 2.6e-16 73..138
RHS_repeat PF05593.16 2.8e-07 859..894
RHS_repeat PF05593.16 6.2e-05 527..562
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-14 MFEAARFGDEISHT
Transmembrane helix 15-37 GALGGFLIGAVLGIALIATVAIA
Inside 38-41 TFTC
Transmembrane helix 42-64 GFGVALLAGLAAGVGGSLLTAAG
Outside 65-1409 EAIGSMFSSPSGTIITASPNVYINNRKAAHVEKSIGACDKHPGPVKIAEGSTNVFINSVAAARKGDKLTCGATISGGSDNVFIGGGRYRYLPVDDEIPGWLRTTVDVLMAVAGAAGGIAQLIKAGTQAGMKAVLPCALKFTAGFVAGEVGSRYVVEPVMRSAIGGLSGNPVDTTTGRKLIPDEIDFSLPGLMPIEWSRFYASNLNVDSVLGKGWVLPWEQSLRKRGSFLYLTDNQGRTVPFVDIAPGQRIYNPHEQVYLVCTQGGHYLLQTLDNIFFYFGEVPDDNKPVPLDRIENALGHFLHFTRTEQGTLTDISATGGIRVHLHYDEVTTRLTSVKRIVDNEAVETLVQYHYDSNGQLAEVFNRNGDSVRRFSYTDGVMTRHSNALGLTCEYRWEIIDGQPRVVEHQTSDGEHFHFRYDREARTTWVTDVLGRELEIHYNKDHRVTSSRDYSGDHYVIEIDDTGNMTGLTLPDGNQLAFEYDEFARLLKETDPLGRSIRYQYHHLTTLITQVDYPDGSTWKANYDAKGNLVAEIDALGHKTEYLNGEDGLPHTIVDPTLKSKYLWWNNLAQVQRFQDCSGKSTYYRYDERHHLVAVTDALNNTTTLERKPDGEVLRINHPDGTHETFTYNELGQVLTHTDGKGQTTQLLRNGRGLPKWRQDAKGQTITYEYDKAIRLVALTNENDATYRFAYDNADRLIEEKRIDNLTRRFSYNLGGHLTQVEEIGYGDKGEQPRRSTHLERDPIGRLLAKLNDDARQDYAYDDGDRLLSIERKPTDTGRKLGITAEKIDFAYDLLGRLVKETTPQGALAYEYDPLSNLTTLTLPTGQHLNHLYYGSGHLHQMNLDGQLISDMERDDLHREIYRTQGKLTSCFGYDSMGRKAWQYATTLPAEKLSQIQNPLIKPERYVEHAYNPIHRRYEYDPAGELSRTLDKLRGETQYEYEANGQLLARNTGRVVDGEEFRYDAAANRLNFNTSRFDHVKDNRLRQWANHEYKYDAWGNLIEKVVGIVRWQTFTYDCENRLVKTETMADTQVESTSSYQYDSLGRRVAKQSEIKGHTEHKRFLWQGLRMLREENPGQSSLYIYEPGSYAPLARVDEKEGEVENKVYYFHTDQIGTPLEMTDAEGQIVWQAKYRAWGAVEKLVVNEVEQNLRFQGQYFDVETGLHYNTFRYYDPEIGRFITQDPIGLLGGYNLYQYAPSAIGWIDPQGLATLFELGTYGGLNGSTHVGDGLQAHELLRHEYLVQQNLAEKSTRLPGNPSIALDLDHHTRGPMKDTRGIGGTHWHEAQIRESQGLGRNEFASSVKRELDITSGALRKSGVPKSRVKELRRMAKKFHSSLNGCG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9775 - -


  Protein sequence: 1410 a.a.    

>T6CP011367 NC_007005:5911246-5915475 [Pseudomonas syringae pv. syringae B728a] [PAAR]
MFEAARFGDEISHTGALGGFLIGAVLGIALIATVAIATFTCGFGVALLAGLAAGVGGSLLTAAGEAIGSMFSSPSGTIIT
ASPNVYINNRKAAHVEKSIGACDKHPGPVKIAEGSTNVFINSVAAARKGDKLTCGATISGGSDNVFIGGGRYRYLPVDDE
IPGWLRTTVDVLMAVAGAAGGIAQLIKAGTQAGMKAVLPCALKFTAGFVAGEVGSRYVVEPVMRSAIGGLSGNPVDTTTG
RKLIPDEIDFSLPGLMPIEWSRFYASNLNVDSVLGKGWVLPWEQSLRKRGSFLYLTDNQGRTVPFVDIAPGQRIYNPHEQ
VYLVCTQGGHYLLQTLDNIFFYFGEVPDDNKPVPLDRIENALGHFLHFTRTEQGTLTDISATGGIRVHLHYDEVTTRLTS
VKRIVDNEAVETLVQYHYDSNGQLAEVFNRNGDSVRRFSYTDGVMTRHSNALGLTCEYRWEIIDGQPRVVEHQTSDGEHF
HFRYDREARTTWVTDVLGRELEIHYNKDHRVTSSRDYSGDHYVIEIDDTGNMTGLTLPDGNQLAFEYDEFARLLKETDPL
GRSIRYQYHHLTTLITQVDYPDGSTWKANYDAKGNLVAEIDALGHKTEYLNGEDGLPHTIVDPTLKSKYLWWNNLAQVQR
FQDCSGKSTYYRYDERHHLVAVTDALNNTTTLERKPDGEVLRINHPDGTHETFTYNELGQVLTHTDGKGQTTQLLRNGRG
LPKWRQDAKGQTITYEYDKAIRLVALTNENDATYRFAYDNADRLIEEKRIDNLTRRFSYNLGGHLTQVEEIGYGDKGEQP
RRSTHLERDPIGRLLAKLNDDARQDYAYDDGDRLLSIERKPTDTGRKLGITAEKIDFAYDLLGRLVKETTPQGALAYEYD
PLSNLTTLTLPTGQHLNHLYYGSGHLHQMNLDGQLISDMERDDLHREIYRTQGKLTSCFGYDSMGRKAWQYATTLPAEKL
SQIQNPLIKPERYVEHAYNPIHRRYEYDPAGELSRTLDKLRGETQYEYEANGQLLARNTGRVVDGEEFRYDAAANRLNFN
TSRFDHVKDNRLRQWANHEYKYDAWGNLIEKVVGIVRWQTFTYDCENRLVKTETMADTQVESTSSYQYDSLGRRVAKQSE
IKGHTEHKRFLWQGLRMLREENPGQSSLYIYEPGSYAPLARVDEKEGEVENKVYYFHTDQIGTPLEMTDAEGQIVWQAKY
RAWGAVEKLVVNEVEQNLRFQGQYFDVETGLHYNTFRYYDPEIGRFITQDPIGLLGGYNLYQYAPSAIGWIDPQGLATLF
ELGTYGGLNGSTHVGDGLQAHELLRHEYLVQQNLAEKSTRLPGNPSIALDLDHHTRGPMKDTRGIGGTHWHEAQIRESQG
LGRNEFASSVKRELDITSGALRKSGVPKSRVKELRRMAKKFHSSLNGCG*

  Nucleotide sequence: 4230 bp    

>T6CP011367 NC_007005:5911246-5915475 [Pseudomonas syringae pv. syringae B728a] [PAAR]
ATGTTCGAGGCCGCGCGCTTTGGTGACGAAATATCGCATACCGGCGCCCTGGGGGGCTTCCTGATCGGGGCTGTGCTCGG
TATTGCGCTCATTGCGACGGTGGCGATCGCAACCTTCACGTGCGGGTTCGGCGTGGCTCTTCTGGCAGGACTTGCAGCAG
GCGTCGGAGGTAGCCTGCTCACCGCCGCAGGAGAGGCGATAGGGAGTATGTTTTCCTCCCCTTCCGGCACGATCATCACG
GCCTCACCCAATGTGTACATCAACAACCGCAAGGCAGCGCATGTTGAAAAAAGCATTGGTGCCTGCGACAAACATCCAGG
GCCGGTCAAGATTGCCGAGGGTTCGACCAACGTCTTCATCAACAGTGTGGCGGCGGCGCGCAAAGGCGACAAGCTGACCT
GTGGCGCGACCATCTCCGGCGGCTCCGACAACGTATTCATCGGCGGCGGCAGGTATCGCTATCTGCCAGTGGACGACGAA
ATTCCAGGCTGGCTGCGCACTACCGTCGATGTGCTGATGGCCGTGGCGGGTGCGGCAGGTGGTATCGCCCAGTTGATCAA
GGCCGGAACGCAGGCAGGCATGAAAGCAGTGCTGCCCTGCGCCCTGAAGTTCACCGCAGGTTTCGTGGCCGGCGAAGTGG
GCAGTCGCTACGTGGTTGAGCCTGTAATGCGCAGTGCGATCGGCGGCCTGAGCGGCAATCCGGTCGACACCACGACTGGC
CGCAAGCTGATCCCGGATGAGATCGACTTCAGCCTGCCAGGCCTGATGCCCATCGAATGGTCGCGTTTTTACGCCAGCAA
TCTGAATGTCGACAGCGTACTCGGCAAAGGCTGGGTGCTGCCGTGGGAGCAAAGCCTGCGCAAGCGCGGCAGCTTCCTCT
ACCTTACGGACAACCAGGGCCGCACCGTACCGTTCGTGGACATAGCGCCCGGCCAGCGCATCTACAACCCTCACGAACAG
GTCTATCTGGTCTGCACGCAGGGTGGGCACTACCTGCTGCAGACGCTGGACAACATCTTCTTCTACTTCGGCGAAGTGCC
TGACGACAACAAGCCGGTCCCGCTGGATCGTATCGAAAACGCACTCGGTCACTTCCTGCATTTCACCCGCACTGAACAAG
GCACGCTGACCGACATCAGCGCGACGGGCGGGATCCGCGTGCACCTGCATTACGACGAAGTGACCACGCGCCTGACCTCG
GTCAAGCGCATCGTCGACAACGAGGCGGTCGAAACGCTGGTTCAATACCACTACGACAGTAATGGCCAGCTCGCTGAGGT
GTTCAACCGCAACGGTGATTCAGTACGCCGTTTCAGTTACACCGATGGCGTCATGACCCGCCACAGCAACGCACTGGGCC
TGACCTGCGAGTACCGCTGGGAAATCATCGACGGCCAGCCGCGTGTCGTCGAACACCAGACCAGCGATGGTGAGCATTTC
CACTTCCGCTACGACCGCGAGGCCCGCACCACCTGGGTCACCGACGTACTGGGCCGCGAGCTGGAAATTCATTACAACAA
GGATCATCGCGTCACCTCGAGCCGCGACTACAGTGGCGATCACTACGTCATCGAGATCGACGACACCGGCAACATGACCG
GGCTGACCCTGCCCGATGGCAACCAGCTGGCATTCGAGTACGACGAGTTCGCCCGATTGCTCAAGGAGACCGATCCGCTG
GGCCGCAGTATTCGCTACCAGTACCACCACCTGACGACGCTGATCACTCAGGTCGACTACCCGGATGGCAGCACCTGGAA
GGCCAACTACGATGCCAAAGGCAATCTCGTGGCCGAGATCGATGCGCTGGGTCACAAGACCGAGTACCTCAACGGTGAGG
ACGGCCTGCCGCACACCATCGTCGACCCGACGCTCAAGTCCAAATACCTGTGGTGGAACAACCTGGCGCAAGTGCAGCGC
TTTCAGGATTGTTCGGGCAAAAGCACTTATTACCGCTATGACGAGCGCCACCACCTAGTGGCCGTCACCGATGCCCTGAA
CAACACCACCACGCTGGAACGCAAGCCCGACGGCGAAGTGCTGCGCATCAACCACCCGGACGGCACACACGAGACCTTTA
CCTACAACGAACTGGGCCAGGTCCTGACCCACACCGATGGCAAAGGCCAGACCACCCAGCTGCTGCGCAACGGCCGTGGC
CTGCCTAAATGGCGACAGGATGCCAAAGGGCAGACCATCACCTACGAATACGACAAAGCCATTCGCCTCGTCGCCCTGAC
CAACGAAAACGATGCGACCTACCGCTTTGCCTACGACAACGCGGACCGTCTGATCGAAGAAAAACGCATCGACAACCTCA
CTCGCCGTTTCAGCTACAACCTCGGCGGGCACCTGACACAGGTCGAGGAAATCGGCTACGGTGATAAAGGCGAACAACCA
CGGCGCAGCACCCATCTGGAGCGCGACCCGATTGGTCGCCTGCTGGCCAAGCTAAACGACGATGCGCGTCAGGACTACGC
CTACGACGACGGCGACCGGCTGCTGTCAATCGAGCGCAAGCCGACGGACACCGGCAGAAAACTGGGCATCACCGCTGAAA
AGATCGACTTCGCCTACGACCTGCTCGGCCGCCTCGTCAAAGAAACCACGCCTCAGGGCGCGCTGGCCTACGAGTACGAT
CCGCTCAGCAACCTGACCACCCTGACCCTGCCCACCGGTCAACACCTGAACCACCTGTATTACGGCAGCGGTCACCTGCA
CCAGATGAATCTGGATGGCCAGCTGATCAGCGACATGGAGCGCGACGATCTCCATCGCGAGATCTATCGCACCCAAGGCA
AGCTCACCAGTTGCTTCGGTTACGACAGCATGGGTCGCAAGGCCTGGCAGTACGCCACAACCTTGCCTGCGGAAAAGCTG
TCACAGATCCAGAACCCGCTCATAAAGCCAGAGCGCTACGTCGAACACGCCTACAACCCGATTCACCGCCGCTACGAATA
CGACCCGGCTGGCGAATTGAGCCGCACCCTCGACAAGCTGCGCGGCGAAACCCAGTACGAATACGAAGCCAACGGCCAGT
TGCTGGCGCGCAACACCGGTCGCGTGGTCGATGGCGAAGAGTTCCGCTACGACGCTGCGGCCAACCGCCTCAACTTCAAC
ACCAGCCGCTTCGACCACGTCAAAGACAACCGCCTCAGACAATGGGCGAACCACGAATACAAATACGACGCCTGGGGCAA
CCTGATCGAAAAAGTCGTCGGCATCGTGCGCTGGCAGACCTTCACCTACGACTGCGAAAACCGTCTGGTCAAAACCGAGA
CCATGGCTGACACGCAGGTGGAAAGCACCAGCAGCTACCAGTACGACAGTTTGGGCCGAAGAGTTGCCAAACAGTCCGAG
ATCAAAGGCCACACCGAACACAAACGCTTCCTCTGGCAAGGCTTGCGCATGCTGCGTGAGGAAAATCCAGGACAGAGCAG
CCTGTATATCTACGAGCCCGGCAGCTATGCGCCGCTGGCGCGCGTGGATGAAAAAGAAGGCGAGGTAGAGAACAAGGTTT
ATTACTTCCACACCGACCAGATCGGCACACCGCTGGAAATGACCGACGCCGAAGGGCAGATCGTCTGGCAGGCCAAGTAC
CGGGCGTGGGGGGCGGTTGAAAAGCTGGTGGTGAACGAGGTCGAGCAGAACCTGCGGTTTCAGGGGCAGTATTTTGATGT
CGAGACGGGGCTGCATTACAACACGTTTAGGTATTATGACCCGGAGATTGGGCGGTTTATAACGCAGGATCCGATTGGGT
TGTTGGGTGGGTATAACCTTTACCAATATGCTCCAAGTGCAATCGGCTGGATTGATCCGCAAGGTTTAGCTACGCTTTTT
GAATTAGGTACCTATGGAGGACTTAACGGGTCAACTCACGTTGGAGATGGTTTACAAGCTCACGAACTCCTCCGTCACGA
ATATCTAGTCCAGCAGAATCTTGCGGAGAAGAGTACCAGGCTTCCAGGAAATCCATCTATTGCGCTGGATCTAGATCATC
ACACTAGAGGCCCAATGAAAGATACAAGAGGTATTGGTGGTACTCATTGGCATGAGGCGCAAATACGTGAGAGCCAAGGT
TTGGGTAGGAATGAATTTGCATCATCTGTAAAGCGTGAGCTAGACATCACTTCAGGCGCGCTACGGAAGAGTGGGGTTCC
TAAGTCTAGAGTGAAAGAGTTAAGAAGAATGGCCAAGAAGTTTCATAGCAGTCTCAATGGGTGTGGGTAA