Detailed information of component protein


Summary

Component ID T6CP000772
Component protein type TssH
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (868 a.a.); Nucleotide sequence (2604 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25745
Coordinate (Strand) 5876740..5879343 (+)
NCBI ID WP_011269330.1
RefSeq NC_007005
Uniprot ID Q4ZLI7_PSEU2, A0A2V4QYK0_PSESY, A0A3M5WR44_9PSED
KEGG ID psb:Psyr_4958
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 4.9e-42 598..765
AAA_lid_9 PF17871.3 4.1e-30 365..463
ClpB_D2-small PF10431.11 2e-12 775..845
AAA PF00004.31 1.3e-08 225..338
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-867 VNLKSLFAKLNETSRTATESAAALCLSENHYDVEVEHLLLQLLDNNDNDLPVILRHYDVVPDRLQAQLVTALGTFKKGNTRTPALSPHITRMIEQAWVLASIEFGQSQIRTGHLVQALLDDDALRRVVIGSAPELEKINADDLRLNMSALVEGSPETKLSKPLANADASTGATTKGNGKTPALDQYTVNLTQSARDGRIDPVLGREFEVRQMVDILTRRRQNNPILTGEAGVGKTAVVEGLALRIVQGDVPSVLKGVAIHTLDLGLLQAGAGVKGEFENRLKSVIEETKRSLHPIILFIDEAHTLIGSGGQAGQNDAANLLKPALARGELRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPDEDKAIHMLRGLLAKMQEHHKVTVMDEALVQAVRLSNRYITGRQLPDKAVSVLDTACARVALGQSAQPGALEDCKRHIDNLNAEITVLEQETAKGSDHARRLTALHEELQAEQETRESLEQQWKRELELVEKLGALSVPQSGEPDAGQIAAVRAELASVQGEQPLVHALVDGGTIGEVISGWTGIPLGKMLRDEIDTVQSLPALLGERVLGQDHALEEIGKRIKISRARMEDPNKPIGVFLLLGPSGVGKTETALALADTLYGGERNVITINMSEYQEAHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVLELFFQVFDKGVLDDGEGREINFRNTVIILTSNTGTDRIMQWCLNTEQKPTPDDIVEGLRDELNQVFKPAFLGRLTIVPYYPVQDAILERIVGLKLERIRKRFERNHQAVLRYDEALIKAIASRCTEVDSGARNIDNILSKTLMPELAQRVLERMAQDKPIQSLTIDLGSDGDFAYTLT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9864 - -


  Protein sequence: 868 a.a.    

>T6CP000772 NC_007005:5876740-5879343 [Pseudomonas syringae pv. syringae B728a] [TssH]
VNLKSLFAKLNETSRTATESAAALCLSENHYDVEVEHLLLQLLDNNDNDLPVILRHYDVVPDRLQAQLVTALGTFKKGNT
RTPALSPHITRMIEQAWVLASIEFGQSQIRTGHLVQALLDDDALRRVVIGSAPELEKINADDLRLNMSALVEGSPETKLS
KPLANADASTGATTKGNGKTPALDQYTVNLTQSARDGRIDPVLGREFEVRQMVDILTRRRQNNPILTGEAGVGKTAVVEG
LALRIVQGDVPSVLKGVAIHTLDLGLLQAGAGVKGEFENRLKSVIEETKRSLHPIILFIDEAHTLIGSGGQAGQNDAANL
LKPALARGELRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPDEDKAIHMLRGLLAKMQEHHKVTVMDEALVQAVRLSN
RYITGRQLPDKAVSVLDTACARVALGQSAQPGALEDCKRHIDNLNAEITVLEQETAKGSDHARRLTALHEELQAEQETRE
SLEQQWKRELELVEKLGALSVPQSGEPDAGQIAAVRAELASVQGEQPLVHALVDGGTIGEVISGWTGIPLGKMLRDEIDT
VQSLPALLGERVLGQDHALEEIGKRIKISRARMEDPNKPIGVFLLLGPSGVGKTETALALADTLYGGERNVITINMSEYQ
EAHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVLELFFQVFDKGVLDDGEGREINFRNTVIILTS
NTGTDRIMQWCLNTEQKPTPDDIVEGLRDELNQVFKPAFLGRLTIVPYYPVQDAILERIVGLKLERIRKRFERNHQAVLR
YDEALIKAIASRCTEVDSGARNIDNILSKTLMPELAQRVLERMAQDKPIQSLTIDLGSDGDFAYTLT*

  Nucleotide sequence: 2604 bp    

>T6CP000772 NC_007005:5876740-5879343 [Pseudomonas syringae pv. syringae B728a] [TssH]
GTGAACCTGAAGTCCCTGTTCGCCAAGCTGAACGAAACGAGCCGCACTGCCACTGAAAGCGCTGCTGCGCTGTGCCTGTC
GGAGAACCATTACGACGTTGAAGTCGAGCACTTGCTGCTGCAACTGCTCGATAACAACGACAACGATCTGCCAGTGATCC
TGCGCCACTACGATGTGGTGCCTGATCGCCTGCAGGCACAGTTGGTCACTGCGCTGGGGACGTTCAAGAAAGGCAACACG
CGGACGCCTGCGCTCTCGCCGCACATCACGCGCATGATCGAGCAGGCTTGGGTGCTGGCATCGATCGAGTTCGGTCAGTC
ACAGATCCGTACCGGCCATCTTGTGCAGGCCTTGCTGGATGACGATGCACTGCGTCGCGTGGTCATCGGCTCGGCGCCGG
AGCTGGAGAAGATCAACGCTGACGACCTGCGCCTGAACATGAGCGCGCTGGTCGAGGGCAGCCCCGAGACCAAGCTGAGC
AAGCCACTGGCGAACGCCGACGCCAGCACGGGCGCCACCACCAAAGGTAACGGCAAGACGCCAGCCCTGGACCAGTACAC
CGTCAACCTGACCCAGAGCGCTCGCGATGGTCGCATTGACCCGGTGCTGGGACGCGAATTCGAAGTGCGGCAGATGGTCG
ACATCCTGACCCGCCGCCGCCAGAACAACCCGATCCTGACCGGTGAAGCCGGCGTCGGTAAAACAGCCGTGGTGGAAGGC
CTGGCGCTGCGCATTGTGCAGGGCGACGTGCCGTCGGTGCTCAAGGGCGTTGCCATTCACACGCTGGATCTGGGCCTGTT
GCAGGCCGGTGCCGGGGTCAAGGGCGAGTTCGAAAACCGCCTCAAGTCGGTCATCGAAGAAACCAAACGCAGCCTGCACC
CGATCATTCTGTTTATCGATGAAGCCCACACGCTGATCGGTTCGGGCGGGCAAGCCGGGCAGAACGATGCCGCCAACCTG
CTCAAGCCTGCCTTGGCACGCGGTGAGCTGCGCACCATCGCCGCCACAACCTGGGCCGAGTACAAAAAGTATTTCGAGAA
GGATGCCGCACTGGCTCGACGCTTCCAGGTGGTGAAAGTCGAAGAGCCTGACGAAGACAAGGCCATCCACATGCTTCGCG
GGTTGCTGGCGAAGATGCAGGAACACCACAAGGTCACGGTGATGGATGAAGCGCTGGTGCAGGCCGTGCGCCTGTCCAAC
CGCTACATCACCGGCCGCCAGTTGCCGGACAAGGCAGTCAGCGTACTCGACACCGCTTGCGCACGCGTTGCCCTGGGCCA
GTCGGCACAGCCGGGAGCGCTGGAGGACTGCAAGCGTCACATCGACAACCTGAACGCGGAAATCACCGTACTGGAGCAGG
AAACCGCCAAGGGCAGCGACCACGCTCGGCGTCTGACGGCCCTGCACGAAGAGCTGCAGGCCGAACAGGAGACCCGCGAG
TCGCTGGAGCAACAGTGGAAGCGCGAGCTGGAGCTGGTCGAGAAGCTTGGCGCACTCAGCGTTCCGCAAAGCGGCGAGCC
TGATGCCGGGCAGATTGCCGCCGTACGCGCCGAACTAGCCAGCGTGCAGGGCGAGCAACCGCTGGTCCACGCACTGGTCG
ATGGCGGCACGATTGGCGAAGTGATTTCCGGCTGGACCGGCATTCCGCTGGGCAAGATGCTGCGCGACGAAATCGACACC
GTGCAGAGCCTGCCCGCGCTGCTGGGCGAACGCGTACTGGGTCAGGACCATGCACTCGAAGAAATCGGCAAGCGCATCAA
GATTTCCCGTGCCCGCATGGAAGACCCGAACAAGCCGATCGGCGTGTTCCTGCTGCTGGGCCCGAGCGGCGTCGGCAAGA
CCGAAACGGCACTGGCCCTGGCTGACACGCTGTATGGCGGCGAACGTAACGTCATCACCATCAACATGTCCGAATACCAG
GAAGCCCACACCGTCTCCAGCCTGAAAGGCTCACCGCCTGGCTACGTCGGTTACGGCGAAGGTGGCGTGCTCACCGAAGC
CGTACGCCGCAAGCCTTATAGCGTGGTGCTGCTCGACGAGGTGGAGAAAGCCCACCCGGATGTGCTCGAGCTGTTTTTCC
AGGTCTTCGACAAAGGCGTGCTGGACGATGGCGAAGGTCGCGAGATCAACTTCCGCAACACCGTGATCATCCTCACCTCC
AACACCGGCACTGATCGCATCATGCAGTGGTGCCTGAACACAGAGCAGAAGCCGACCCCCGATGACATCGTGGAAGGCCT
GCGCGACGAGCTGAATCAGGTGTTCAAACCGGCCTTCCTGGGTCGCCTGACCATCGTGCCGTATTACCCGGTACAGGACG
CGATTCTGGAGCGCATCGTCGGCCTGAAGCTGGAGCGCATTCGCAAGCGTTTCGAGCGCAATCATCAGGCGGTGCTGCGT
TACGACGAAGCACTGATAAAGGCTATCGCGTCGCGCTGCACGGAAGTCGACAGCGGCGCGCGCAATATCGACAACATCCT
GTCCAAGACCCTCATGCCGGAGCTCGCTCAGCGGGTACTGGAGCGGATGGCGCAGGACAAGCCGATTCAGAGCCTGACCA
TCGACCTGGGCAGCGATGGCGACTTCGCTTACACCCTGACCTGA