Detailed information of component protein


Summary

Component ID T6CP000768
Component protein type TssC
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (501 a.a.); Nucleotide sequence (1503 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25725
Coordinate (Strand) 5871865..5873367 (+)
NCBI ID WP_003343625.1
RefSeq NC_007005
Uniprot ID A0A7V8LV91_PSESX, A0A3M3A3J5_PSEA0, A0A2G4DBQ6_PSEA0, A0A0N0F569_PSEA0, A0A0P9GQT7_PSESX, A0A0L8IJR5_PSESX, A0A0P9SWG4_PSESX, Q4ZLJ1_PSEU2, A0A2V3Q7D3_9PSED, A0A3M4Y0M8_9PSED, A0A0N1JAR8_PSEA0, A0A554GUZ4_9PSED, A0A0P9NNF1_PSESX, A0A0P9SHH2_PSESX, A0A0P9LRR1_PSEA0, Q48Q73_PSE14, A0A3M5FGP9_PSESS, A0A2K4WN16_PSESX, A0A0P9R1U5_PSESG, A0A0P9WIX5_PSESH, A0A2S4J473_PSESY, A0A0N8T1J5_PSEAJ, A0A0P9WJ08_PSEA0, A0A0Q0B472_PSESS, A0A0P9VYQ0_PSESS, A0A0P9QQ73_9PSED, A0A0P9SWK9_PSEAV, A0A2V4Q250_PSESJ, A0A0N0FV96_PSEYM, A0A3M5WR22_9PSED, A0A0Q0C4S6_PSEAP, A0A0N0VQU9_PSEAQ, E7PH89_PSESG
KEGG ID psb:Psyr_4954, psp:PSPPH_0133
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 5.5e-142 73..374
VipB_2 PF18945.2 2.2e-44 384..494
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-500 MTDKQPVTQQDSALVEVENFAPQSSLLDSIISESRVARSETERTRTRDLIGELVAQVLEGEMTPSKDLIAVLDARIAEIDSMLSEQMNEIMHAREFQQLEASWRGLKYQVDQTETSTTLKIHLLNASKKDLVRDLKASSEFDQSALFKKIYEEEYGTFGGAPFGMLLGDYEFNRSPEDMYLLEEISHVAAAAHAPFISAASAELFGWDSFTDMAGPRDLAKIFDTVEYAKWKSFRASEDSRYVGLTLPHVLGRLPYGPDTTPVEEFNFVESVDGRDHNKYLWMNAAYALGTRVTDAFSRYGWCVAIRGVEGGGLVEGLPTHTFKTDDGEIALKCPTEIAITDRREKELSDLGFIPLVHCKGTDYAAFFGTQSTQKQKQYNTDIANANARLSAQLQYIFATSRIAHYMKAIMRDKIGSFASRKDVELFLNKWLSSYVLLDDTASQEAKAKFPLREARAEVFEVPGKPGVYKAVTYLRPHYQLDELTASLRLVAELPQSTRG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9892 - -


  Protein sequence: 501 a.a.    

>T6CP000768 NC_007005:5871865-5873367 [Pseudomonas syringae pv. syringae B728a] [TssC]
MTDKQPVTQQDSALVEVENFAPQSSLLDSIISESRVARSETERTRTRDLIGELVAQVLEGEMTPSKDLIAVLDARIAEID
SMLSEQMNEIMHAREFQQLEASWRGLKYQVDQTETSTTLKIHLLNASKKDLVRDLKASSEFDQSALFKKIYEEEYGTFGG
APFGMLLGDYEFNRSPEDMYLLEEISHVAAAAHAPFISAASAELFGWDSFTDMAGPRDLAKIFDTVEYAKWKSFRASEDS
RYVGLTLPHVLGRLPYGPDTTPVEEFNFVESVDGRDHNKYLWMNAAYALGTRVTDAFSRYGWCVAIRGVEGGGLVEGLPT
HTFKTDDGEIALKCPTEIAITDRREKELSDLGFIPLVHCKGTDYAAFFGTQSTQKQKQYNTDIANANARLSAQLQYIFAT
SRIAHYMKAIMRDKIGSFASRKDVELFLNKWLSSYVLLDDTASQEAKAKFPLREARAEVFEVPGKPGVYKAVTYLRPHYQ
LDELTASLRLVAELPQSTRG*

  Nucleotide sequence: 1503 bp    

>T6CP000768 NC_007005:5871865-5873367 [Pseudomonas syringae pv. syringae B728a] [TssC]
ATGACCGATAAGCAACCCGTGACTCAGCAGGACAGCGCTCTGGTTGAAGTGGAAAACTTCGCCCCGCAATCTTCCTTGCT
CGACAGCATCATTTCCGAAAGCCGCGTTGCACGCTCCGAAACCGAGCGCACCCGTACCCGCGATCTGATCGGCGAACTGG
TGGCCCAGGTACTTGAAGGCGAGATGACGCCGAGCAAGGACCTGATTGCCGTGCTCGACGCGCGCATCGCCGAGATCGAT
TCGATGCTCTCCGAGCAAATGAACGAAATCATGCACGCCCGTGAATTCCAGCAGCTGGAAGCATCCTGGCGCGGCCTGAA
GTATCAGGTTGACCAGACCGAGACCAGCACCACGCTGAAGATCCACCTGCTCAACGCGTCGAAAAAAGACCTCGTGCGTG
ACCTCAAGGCATCGTCCGAATTCGACCAGAGCGCACTGTTCAAGAAGATCTACGAAGAAGAGTACGGCACCTTCGGTGGT
GCGCCGTTCGGCATGCTGCTGGGTGACTACGAGTTCAACCGCAGCCCGGAAGACATGTACCTGCTGGAAGAGATCTCCCA
CGTGGCAGCTGCCGCCCACGCGCCGTTCATTTCGGCTGCATCGGCTGAACTGTTCGGCTGGGACTCGTTCACCGACATGG
CCGGCCCTCGCGACCTGGCGAAGATCTTCGACACCGTCGAATACGCCAAGTGGAAGTCGTTCCGCGCCTCCGAAGACTCC
CGCTATGTCGGCCTGACCCTGCCGCACGTGCTGGGTCGCCTGCCGTATGGCCCGGATACCACGCCTGTCGAAGAATTCAA
CTTCGTCGAAAGCGTCGATGGCCGTGACCACAACAAATACCTGTGGATGAACGCTGCCTACGCGCTGGGCACTCGTGTGA
CCGACGCATTCTCCCGCTACGGCTGGTGCGTGGCGATCCGTGGTGTGGAAGGCGGTGGTCTGGTCGAAGGCCTGCCGACT
CATACCTTCAAGACCGATGACGGTGAAATCGCGCTCAAATGCCCGACTGAAATCGCCATCACCGACCGTCGCGAAAAAGA
GCTCTCGGACCTCGGCTTCATTCCGCTGGTGCACTGCAAAGGTACCGACTACGCCGCGTTCTTCGGCACCCAGTCGACCC
AGAAGCAGAAGCAGTACAACACCGACATTGCCAACGCCAACGCACGTCTGTCGGCGCAGCTGCAATACATCTTCGCGACC
TCGCGTATCGCTCACTACATGAAAGCGATCATGCGCGACAAGATCGGCAGTTTTGCATCGCGCAAGGATGTCGAGCTGTT
CCTCAACAAGTGGCTGTCCAGCTACGTGTTGCTGGATGACACCGCCAGCCAGGAAGCCAAGGCCAAGTTCCCGCTGCGCG
AGGCGCGTGCCGAAGTGTTCGAAGTCCCTGGCAAGCCTGGCGTCTACAAGGCCGTGACTTACCTGCGTCCGCATTACCAA
CTGGATGAACTGACGGCATCGCTGCGTCTGGTCGCCGAATTGCCGCAATCGACACGCGGCTAA