Detailed information of regulator


Summary

Regulator ID REG0151 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (665 a.a.); Nucleotide sequence (1995 bp)
Description Results of the qRT-PCR showing icmF transcript levels were 3.3-fold lower in the B728a ladS mutant than in wild-type B728a.
Reference
External database links

Locus tag (Gene) Psyr_4339 (ladS)
Coordinates (Strand) 5191591..5193585 (-)
NCBI ID 639463044
RefSeq NC_007005
Uniprot ID UPI0000395496
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcription ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
7TMR-DISM_7TM PF07695.13 6.8e-52 184..386
7TMR-DISMED2 PF07696.13 8.6e-39 32..171
HATPase_c PF02518.28 5.3e-22 530..644
HisKA PF00512.27 6.3e-12 419..483
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-182 MRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSEGAVQAPLYLWSTHAYMDAQPTK
Transmembrane helix 183-205 IYVFGLIYGVLLGMLVYNLFIYL
Inside 206-211 SVREVD
Transmembrane helix 212-234 YLYYLLYVASFGLYQMSINGVAI
Outside 235-248 EYLWPDSPWWANAS
Transmembrane helix 249-268 TPFLISLATLFACQFSRSFL
Inside 269-279 GTAQLSRWLDR
Transmembrane helix 280-302 LLLAVIGAAVLVMGMALFLSYGP
Outside 303-306 ALRG
Transmembrane helix 307-329 AAQLVMAGALTIYLAGIVAVIKG
Inside 330-335 ERVGRY
Transmembrane helix 336-358 FVLAWSVFMVSGLIFGLMLLGYL
Outside 359-361 PNT
Transmembrane helix 362-384 FLTMYASHIGTVLEMAFLSMALA
Inside 385-664 DRINHARRQQAQTLLAAGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDMMSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTLSVPMLMVVPGAVRRAPELKAQWGI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9958 pos: 18-19. SHA-VE. MRYLLIILALWLPLQSHA


  Protein sequence of regulator: 665 a.a.    .

>REG0151 NC_007005:c5193585-5191591 [Pseudomonas syringae pv. syringae B728a]
MRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVE
LTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSE
GAVQAPLYLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQMSINGVAIEYLWPD
SPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGAAVLVMGMALFLSYGPALRGAAQLVMAGALTIYL
AGIVAVIKGERVGRYFVLAWSVFMVSGLIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRINHARRQQAQTLLA
AGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDMMSMINGILTLTEL
QAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFS
GHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTL
SVPMLMVVPGAVRRAPELKAQWGI*

  Nucleotide sequence of regulator: 1995 bp    .

>REG0151 NC_007005:c5193585-5191591 [Pseudomonas syringae pv. syringae B728a]
ATGCGCTATCTGTTGATCATTCTGGCTCTTTGGCTGCCGTTGCAGAGTCATGCAGTCGAGTTTGATGAAAACACTCGTTT
TCTGCCGCTGGGCCGTGCCATTCAGGTATTCGAGGACCCCACCGGCGAGGCGACCATCGAAAGCGTCAGCGCTGCCGCTC
ATGCCAGTGCCTTTAAGCCCGTGCCTCCCGGAACCTTCAACGCCGGCTATTCGCGCTCTGCCTTCTGGCTGAAGGTCGAG
CTGACCTATCGCCCCACAGATGCCAGCATCCATAACGACTGGCTGCTGGAACTGGCCTATCCGCCAATGGACCACATTGA
CTTCTACGGCCCGGATGCCGACGGGCAGCCCACTCGCGCCTGGCATACCGGCGATATGCTGCCGTTCTCCAGCCGTCAGT
TCGCCCAGAACAACTACCTCTTCCAGCTGGACCTGCCGCCTGGCCAGACGCGAACCCTGTACATGCGTATTCGCAGCGAA
GGCGCTGTTCAGGCACCGCTCTACCTCTGGTCCACCCACGCCTATATGGATGCGCAGCCCACCAAGATTTACGTCTTCGG
TCTGATCTACGGCGTGCTGCTGGGCATGCTGGTCTACAACCTGTTTATCTACCTCAGCGTTCGTGAGGTGGATTACCTGT
ATTACCTGCTGTATGTCGCCTCGTTCGGGCTTTACCAGATGTCGATCAACGGCGTTGCGATCGAATACCTATGGCCTGAC
AGCCCGTGGTGGGCGAATGCGTCGACCCCCTTCCTGATATCGCTGGCGACGTTATTTGCGTGCCAGTTTTCGCGCAGCTT
CCTGGGCACGGCACAACTGAGCCGCTGGCTGGACCGGCTGTTGCTGGCGGTCATCGGCGCTGCCGTTCTGGTCATGGGTA
TGGCCTTGTTCCTCAGCTACGGGCCAGCCTTGCGCGGCGCCGCTCAACTGGTCATGGCAGGCGCCCTGACGATCTACCTG
GCGGGTATCGTTGCGGTCATCAAAGGCGAGCGGGTCGGGCGCTATTTTGTGCTCGCCTGGTCGGTGTTCATGGTCAGCGG
ACTGATCTTCGGCTTGATGCTGCTTGGCTATCTGCCTAATACCTTCCTGACCATGTACGCCTCGCACATCGGTACGGTGC
TGGAAATGGCGTTTCTGTCGATGGCGCTGGCCGACCGTATCAACCACGCCCGCAGGCAGCAGGCGCAAACCTTGCTGGCT
GCCGGTCAGGATCTGGAGCGTCTCAACCAGCAACTGGCCAACAGCAACCGGCTTAAGGATGAGTTTCTGGCCACGCTCAC
CCATGAGCTGCGCACGCCGATGAACGGCGTGATCGGGTCGCTGGAAGTCATGCAGACGCTCGACATGGACGACGAAGTCG
AGATGTATCAGCAAACCGCCGCCAGTTCGGCACGCGACATGATGAGCATGATCAACGGCATCCTGACCCTCACCGAGCTG
CAGGCCGGGGTGCTGTATGCCGACTGCGAGGCGTTTTCGCCCAAGGATCTGGCCGACAGGCTGCGTGAACGCTTCAGCGG
TGCGGCTCAGTCCAAGGGCCTGATATTGACACTTGATCTGGACGATAAACTGCCGCCGAGCCTTCGTGGCGACGCGGGCA
AGCTCTACCAGTGCATCGAGTGCCTGGTGGACAACGCGATCAAATTCACCCGAAAAGGCGCAGTGCAGGTGCGTTTCAGT
GGTCATCCGCAAGACCTGGAGCGCCTGTACCTGCGGGTCGAAGTGATGGACAGCGGTATCGGTTTGAGCTGCCTTGACGA
AGCCGGGCTGTATCAGCATTTCTTTCAGGTCGACGGCTCCATGACCCGTGAGTACGGTGGGCTGGGCATCGGTCTGGCGA
TCTGCCGGAAACTGATCGAGTTGCAGGGCGGCGAGCTGAGTCATCGTTCCGAACCCGGCAAAGGCAGCTGTTTTACGCTG
AGTGTCCCGATGCTGATGGTCGTACCGGGTGCAGTGCGCAGGGCGCCTGAACTCAAGGCTCAATGGGGCATATAA