Detailed information of regulator


Summary

Regulator ID REG0151 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (665 a.a.); Nucleotide sequence (1995 bp)
Description Results of the qRT-PCR showing icmF transcript levels were 3.3-fold lower in the B728a ladS mutant than in wild-type B728a.
Reference
External database links

Locus tag (Gene) Psyr_4339 (ladS)
Coordinates (Strand) 5191591..5193585 (-)
NCBI ID 639463044
RefSeq NC_007005
Uniprot ID UPI0000395496
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
T6SS transcription↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
7TMR-DISM_7TMPF07695.136.8e-52184..386
7TMR-DISMED2PF07696.138.6e-3932..171
HATPase_cPF02518.285.3e-22530..644
HisKAPF00512.276.3e-12419..483
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-182MRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVELTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSEGAVQAPLYLWSTHAYMDAQPTK
Transmembrane helix183-205IYVFGLIYGVLLGMLVYNLFIYL
Inside206-211SVREVD
Transmembrane helix212-234YLYYLLYVASFGLYQMSINGVAI
Outside235-248EYLWPDSPWWANAS
Transmembrane helix249-268TPFLISLATLFACQFSRSFL
Inside269-279GTAQLSRWLDR
Transmembrane helix280-302LLLAVIGAAVLVMGMALFLSYGP
Outside303-306ALRG
Transmembrane helix307-329AAQLVMAGALTIYLAGIVAVIKG
Inside330-335ERVGRY
Transmembrane helix336-358FVLAWSVFMVSGLIFGLMLLGYL
Outside359-361PNT
Transmembrane helix362-384FLTMYASHIGTVLEMAFLSMALA
Inside385-664DRINHARRQQAQTLLAAGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDMMSMINGILTLTELQAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFSGHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTLSVPMLMVVPGAVRRAPELKAQWGI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
SP(Sec/SPI)0.9958pos: 18-19. SHA-VE.MRYLLIILALWLPLQSHA


  Protein sequence of regulator: 665 a.a.    .

>REG0151 NC_007005:c5193585-5191591 [Pseudomonas syringae pv. syringae B728a]
MRYLLIILALWLPLQSHAVEFDENTRFLPLGRAIQVFEDPTGEATIESVSAAAHASAFKPVPPGTFNAGYSRSAFWLKVE
LTYRPTDASIHNDWLLELAYPPMDHIDFYGPDADGQPTRAWHTGDMLPFSSRQFAQNNYLFQLDLPPGQTRTLYMRIRSE
GAVQAPLYLWSTHAYMDAQPTKIYVFGLIYGVLLGMLVYNLFIYLSVREVDYLYYLLYVASFGLYQMSINGVAIEYLWPD
SPWWANASTPFLISLATLFACQFSRSFLGTAQLSRWLDRLLLAVIGAAVLVMGMALFLSYGPALRGAAQLVMAGALTIYL
AGIVAVIKGERVGRYFVLAWSVFMVSGLIFGLMLLGYLPNTFLTMYASHIGTVLEMAFLSMALADRINHARRQQAQTLLA
AGQDLERLNQQLANSNRLKDEFLATLTHELRTPMNGVIGSLEVMQTLDMDDEVEMYQQTAASSARDMMSMINGILTLTEL
QAGVLYADCEAFSPKDLADRLRERFSGAAQSKGLILTLDLDDKLPPSLRGDAGKLYQCIECLVDNAIKFTRKGAVQVRFS
GHPQDLERLYLRVEVMDSGIGLSCLDEAGLYQHFFQVDGSMTREYGGLGIGLAICRKLIELQGGELSHRSEPGKGSCFTL
SVPMLMVVPGAVRRAPELKAQWGI*

  Nucleotide sequence of regulator: 1995 bp    .

>REG0151 NC_007005:c5193585-5191591 [Pseudomonas syringae pv. syringae B728a]
ATGCGCTATCTGTTGATCATTCTGGCTCTTTGGCTGCCGTTGCAGAGTCATGCAGTCGAGTTTGATGAAAACACTCGTTT
TCTGCCGCTGGGCCGTGCCATTCAGGTATTCGAGGACCCCACCGGCGAGGCGACCATCGAAAGCGTCAGCGCTGCCGCTC
ATGCCAGTGCCTTTAAGCCCGTGCCTCCCGGAACCTTCAACGCCGGCTATTCGCGCTCTGCCTTCTGGCTGAAGGTCGAG
CTGACCTATCGCCCCACAGATGCCAGCATCCATAACGACTGGCTGCTGGAACTGGCCTATCCGCCAATGGACCACATTGA
CTTCTACGGCCCGGATGCCGACGGGCAGCCCACTCGCGCCTGGCATACCGGCGATATGCTGCCGTTCTCCAGCCGTCAGT
TCGCCCAGAACAACTACCTCTTCCAGCTGGACCTGCCGCCTGGCCAGACGCGAACCCTGTACATGCGTATTCGCAGCGAA
GGCGCTGTTCAGGCACCGCTCTACCTCTGGTCCACCCACGCCTATATGGATGCGCAGCCCACCAAGATTTACGTCTTCGG
TCTGATCTACGGCGTGCTGCTGGGCATGCTGGTCTACAACCTGTTTATCTACCTCAGCGTTCGTGAGGTGGATTACCTGT
ATTACCTGCTGTATGTCGCCTCGTTCGGGCTTTACCAGATGTCGATCAACGGCGTTGCGATCGAATACCTATGGCCTGAC
AGCCCGTGGTGGGCGAATGCGTCGACCCCCTTCCTGATATCGCTGGCGACGTTATTTGCGTGCCAGTTTTCGCGCAGCTT
CCTGGGCACGGCACAACTGAGCCGCTGGCTGGACCGGCTGTTGCTGGCGGTCATCGGCGCTGCCGTTCTGGTCATGGGTA
TGGCCTTGTTCCTCAGCTACGGGCCAGCCTTGCGCGGCGCCGCTCAACTGGTCATGGCAGGCGCCCTGACGATCTACCTG
GCGGGTATCGTTGCGGTCATCAAAGGCGAGCGGGTCGGGCGCTATTTTGTGCTCGCCTGGTCGGTGTTCATGGTCAGCGG
ACTGATCTTCGGCTTGATGCTGCTTGGCTATCTGCCTAATACCTTCCTGACCATGTACGCCTCGCACATCGGTACGGTGC
TGGAAATGGCGTTTCTGTCGATGGCGCTGGCCGACCGTATCAACCACGCCCGCAGGCAGCAGGCGCAAACCTTGCTGGCT
GCCGGTCAGGATCTGGAGCGTCTCAACCAGCAACTGGCCAACAGCAACCGGCTTAAGGATGAGTTTCTGGCCACGCTCAC
CCATGAGCTGCGCACGCCGATGAACGGCGTGATCGGGTCGCTGGAAGTCATGCAGACGCTCGACATGGACGACGAAGTCG
AGATGTATCAGCAAACCGCCGCCAGTTCGGCACGCGACATGATGAGCATGATCAACGGCATCCTGACCCTCACCGAGCTG
CAGGCCGGGGTGCTGTATGCCGACTGCGAGGCGTTTTCGCCCAAGGATCTGGCCGACAGGCTGCGTGAACGCTTCAGCGG
TGCGGCTCAGTCCAAGGGCCTGATATTGACACTTGATCTGGACGATAAACTGCCGCCGAGCCTTCGTGGCGACGCGGGCA
AGCTCTACCAGTGCATCGAGTGCCTGGTGGACAACGCGATCAAATTCACCCGAAAAGGCGCAGTGCAGGTGCGTTTCAGT
GGTCATCCGCAAGACCTGGAGCGCCTGTACCTGCGGGTCGAAGTGATGGACAGCGGTATCGGTTTGAGCTGCCTTGACGA
AGCCGGGCTGTATCAGCATTTCTTTCAGGTCGACGGCTCCATGACCCGTGAGTACGGTGGGCTGGGCATCGGTCTGGCGA
TCTGCCGGAAACTGATCGAGTTGCAGGGCGGCGAGCTGAGTCATCGTTCCGAACCCGGCAAAGGCAGCTGTTTTACGCTG
AGTGTCCCGATGCTGATGGTCGTACCGGGTGCAGTGCGCAGGGCGCCTGAACTCAAGGCTCAATGGGGCATATAA