Detailed information of component protein


Summary

Component ID T6CP000777
Component protein type TssM
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (831 a.a.); Nucleotide sequence (2493 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25775
Coordinate (Strand) 5887089..5889581 (+)
NCBI ID WP_011269333.1
RefSeq NC_007005
Uniprot ID Q4ZLI1_PSEU2, A0A2V4QDX2_PSESY
KEGG ID psb:Psyr_4964
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 5.9e-71 188..444
OmpA PF00691.22 2e-13 715..808
IcmF-related PF06761.14 5.7e-13 510..672
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MNKIKYYFLRYQ
Transmembrane helix 13-35 PYVLGVCFFLAIFIVWSIGRALG
Outside 36-38 FSS
Transmembrane helix 39-61 MNSLLTGIGLFLLLSAAYVLLLY
Inside 62-442 RGVSQHQNLEGLLRDDADQAVLNASPADREEVSLLRERLLQSLERLQSNKPNGSNSKDALYALPWYLVVGQPAAGKSTMLYQSGLNFPYAEREGARVAGLGGTRNCDWFFSSEAVLLDTAGRYMDDQEEAGKWRAFLQLLRQHRQRRPLNGLIVTVSIHDILQSSVEDQERIAKRLRERIQETHALLEVRLPVYLVFTKCDLVPGFVPFYRQLDDVARGEVMGKTFPHKGFEQADWGQRFGVAMDELINYWKQIANQQLVLQDIQVTRQDTAVYRFPLELEALKPRLLLFVDALLRANPYQSAEMLRGFYFTSALDADQPEMGGHTRQVTERFQLESEQEAVDGNVQPHPLFINSLFRKVIIPDQHLVALYTTNHRERRRK
Transmembrane helix 443-465 AAWIGGASLVALLLCSLWGWSYW
Outside 466-830 NNKDNIQSISSELAQAAAEDSKATGQYTEWQTLDRMRFWTSLYYDRHHDEGVPLRLRLGLYRGYDVEPLLRTRYFNRLETVMLKPTADNLTRSLYLLTTIKVYQRNAKDLMPVTGVDSVEPKALPPDNRAQSIAAFGKATLDTYVMLSRAQREKADPAFLKAKMPDYWYPAIARQTGKTITANAEAGSNQDYEYASRQINFYSDQIHELDVPRILDNAFLTSSSRNYINSLLTQSLKAIETITLESDTLFAFGRADFQSLKTEGQNQLSAIAGKLLSTPNIGKIVIAGHADQLGDPQSNMQVSKQRAQTIKTYLVGKGVPAELVDAIGEGSEKPLVKCDMQQPRAQLIQCLEPNRRVEIEVRALN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9920 - -


  Protein sequence: 831 a.a.    

>T6CP000777 NC_007005:5887089-5889581 [Pseudomonas syringae pv. syringae B728a] [TssM]
MNKIKYYFLRYQPYVLGVCFFLAIFIVWSIGRALGFSSMNSLLTGIGLFLLLSAAYVLLLYRGVSQHQNLEGLLRDDADQ
AVLNASPADREEVSLLRERLLQSLERLQSNKPNGSNSKDALYALPWYLVVGQPAAGKSTMLYQSGLNFPYAEREGARVAG
LGGTRNCDWFFSSEAVLLDTAGRYMDDQEEAGKWRAFLQLLRQHRQRRPLNGLIVTVSIHDILQSSVEDQERIAKRLRER
IQETHALLEVRLPVYLVFTKCDLVPGFVPFYRQLDDVARGEVMGKTFPHKGFEQADWGQRFGVAMDELINYWKQIANQQL
VLQDIQVTRQDTAVYRFPLELEALKPRLLLFVDALLRANPYQSAEMLRGFYFTSALDADQPEMGGHTRQVTERFQLESEQ
EAVDGNVQPHPLFINSLFRKVIIPDQHLVALYTTNHRERRRKAAWIGGASLVALLLCSLWGWSYWNNKDNIQSISSELAQ
AAAEDSKATGQYTEWQTLDRMRFWTSLYYDRHHDEGVPLRLRLGLYRGYDVEPLLRTRYFNRLETVMLKPTADNLTRSLY
LLTTIKVYQRNAKDLMPVTGVDSVEPKALPPDNRAQSIAAFGKATLDTYVMLSRAQREKADPAFLKAKMPDYWYPAIARQ
TGKTITANAEAGSNQDYEYASRQINFYSDQIHELDVPRILDNAFLTSSSRNYINSLLTQSLKAIETITLESDTLFAFGRA
DFQSLKTEGQNQLSAIAGKLLSTPNIGKIVIAGHADQLGDPQSNMQVSKQRAQTIKTYLVGKGVPAELVDAIGEGSEKPL
VKCDMQQPRAQLIQCLEPNRRVEIEVRALN*

  Nucleotide sequence: 2493 bp    

>T6CP000777 NC_007005:5887089-5889581 [Pseudomonas syringae pv. syringae B728a] [TssM]
ATGAACAAGATCAAATACTACTTTTTGCGCTACCAGCCCTACGTTCTAGGGGTCTGCTTTTTCCTGGCGATCTTCATCGT
CTGGAGCATCGGCCGTGCCCTGGGCTTCAGCTCGATGAACAGTCTGCTGACGGGGATCGGCCTGTTTCTGCTGTTGTCGG
CAGCCTATGTGCTGTTGCTGTACCGAGGTGTATCACAGCACCAGAACCTGGAAGGCCTGCTGCGTGACGACGCCGATCAG
GCGGTGCTCAACGCCAGCCCGGCAGACCGTGAAGAAGTCAGCCTGCTGCGCGAGCGCCTGCTGCAAAGCCTCGAGCGCCT
GCAGAGCAACAAACCGAACGGCAGCAACTCCAAGGACGCGCTCTACGCCCTGCCCTGGTATCTGGTGGTCGGTCAGCCCG
CAGCGGGCAAAAGCACCATGCTCTATCAGTCGGGCCTCAACTTCCCCTACGCAGAGCGCGAAGGCGCACGCGTGGCCGGG
TTGGGTGGCACGCGCAATTGTGACTGGTTCTTCAGTTCGGAAGCCGTGCTGCTGGACACGGCCGGTCGCTACATGGACGA
TCAGGAAGAAGCCGGCAAATGGCGCGCTTTTCTGCAATTGCTGCGTCAGCACCGCCAGCGCAGACCGCTCAACGGCCTGA
TTGTCACCGTCAGCATTCATGACATCCTGCAGTCCTCGGTGGAGGACCAGGAGCGCATCGCCAAACGTCTGCGCGAGCGC
ATCCAGGAAACCCACGCGCTGCTGGAAGTGCGTCTGCCGGTGTATCTGGTGTTCACCAAGTGCGACCTGGTCCCGGGCTT
CGTGCCGTTCTATCGCCAGCTTGACGATGTGGCGCGTGGCGAAGTGATGGGCAAGACCTTCCCGCACAAGGGCTTCGAGC
AAGCCGACTGGGGCCAGCGCTTCGGTGTGGCGATGGATGAACTGATCAATTACTGGAAACAGATCGCCAACCAGCAACTG
GTGCTTCAGGACATCCAGGTCACTCGTCAGGACACCGCGGTGTATCGCTTCCCGCTGGAGCTGGAAGCCTTGAAACCGCG
CCTGCTCCTGTTCGTCGATGCTCTGCTGCGCGCCAACCCGTATCAAAGCGCCGAGATGCTGCGCGGTTTCTACTTCACCA
GCGCACTGGACGCCGATCAGCCCGAGATGGGCGGACACACCCGCCAGGTCACTGAGCGATTCCAGCTGGAAAGCGAGCAG
GAAGCTGTCGATGGCAATGTCCAGCCGCACCCGCTGTTCATCAACAGCCTGTTCCGCAAGGTAATCATCCCCGATCAGCA
TCTGGTGGCGCTGTACACCACCAACCACCGCGAGCGGCGCCGCAAGGCCGCCTGGATCGGTGGCGCAAGCCTTGTGGCGT
TGCTGCTGTGCAGTCTGTGGGGCTGGTCGTACTGGAACAACAAGGACAACATCCAGTCCATCTCCAGCGAACTCGCTCAG
GCCGCAGCCGAGGACAGCAAGGCCACCGGGCAGTACACCGAGTGGCAAACGCTGGATCGCATGCGCTTCTGGACTTCGCT
TTACTACGATCGTCATCACGACGAAGGCGTACCACTGCGCTTGCGTCTGGGTCTCTACCGGGGCTATGACGTCGAGCCGC
TGCTGCGCACGCGTTACTTCAACCGTCTGGAAACGGTGATGCTCAAACCGACCGCAGACAACCTGACGCGCTCGCTTTAC
CTGCTGACCACCATCAAGGTCTACCAGCGTAATGCCAAGGACCTGATGCCGGTCACCGGCGTCGACAGTGTCGAGCCCAA
GGCGCTGCCGCCGGATAACCGCGCGCAATCCATCGCCGCCTTCGGCAAGGCCACGCTGGACACTTACGTGATGTTGTCGC
GCGCCCAGCGTGAAAAGGCCGACCCGGCGTTCCTGAAAGCGAAGATGCCTGATTACTGGTACCCGGCCATCGCGCGTCAG
ACTGGCAAGACCATCACCGCGAATGCCGAGGCAGGCTCCAATCAGGATTACGAGTACGCCAGCCGACAGATCAACTTCTA
CAGCGATCAGATTCACGAGCTGGACGTGCCACGGATTCTCGACAACGCATTTCTGACATCCAGCTCGCGTAATTACATCA
ACAGCCTGTTGACGCAGTCGTTGAAGGCTATCGAAACGATCACGCTGGAATCGGACACGCTGTTTGCCTTTGGTCGTGCC
GATTTCCAGAGCCTTAAAACCGAAGGTCAAAATCAGTTGAGCGCGATCGCCGGCAAGCTGTTGAGTACACCGAACATCGG
CAAGATCGTGATTGCAGGTCACGCCGACCAGTTGGGCGACCCGCAAAGCAACATGCAGGTGTCCAAACAGCGTGCGCAGA
CCATCAAGACTTACCTGGTCGGCAAGGGCGTGCCCGCCGAGCTGGTGGACGCGATCGGTGAAGGCAGCGAGAAGCCTCTG
GTCAAATGCGACATGCAGCAACCCAGAGCGCAGTTGATTCAGTGCCTTGAGCCGAATCGACGCGTGGAGATCGAAGTACG
CGCGCTCAATTGA