Detailed information of component protein


Summary

Component ID T6CP000775
Component protein type TssL
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (238 a.a.); Nucleotide sequence (714 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25760
Coordinate (Strand) 5881461..5882174 (+)
NCBI ID WP_003318805.1
RefSeq NC_007005
Uniprot ID A0A1H0KS23_9PSED, A0A0N8Q953_PSESX, A0A3M5FEE4_PSESX, A0A0P9XHP3_PSESX, A0A0P9PL68_9PSED, Q4ZLI4_PSEU2, A0A326SP38_9PSED, A0A2V3Q735_9PSED, A0A3M4XZI9_9PSED, A0A1G9X8E0_PSESX, A0A7Z6UTU6_PSESH, A0A2S4J459_PSESY, A0A2V4Q4W3_PSESJ, A0A3M5WRC7_9PSED, A0A0Q0CVB5_PSEAP
KEGG ID psb:Psyr_4961
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.4e-52 19..218
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-197 MTEAVMQGAATAATEKPTLKDLVRDFITMALIVRRGRQAISVTAFESSVDTFFANLERQARSANYSVEQVKDAQYALCAFLDESVLRSGDNDMRRHFEMEPLQFRYFGVHLAGEGFFEKIELLRADVKKNLDVLEVYHLCLALGFEGKFGLGQKDQLRYLANTLGQDIARYRKTPKALSPDWALPDQVSQMLRHEVP
Transmembrane helix 198-220 VWLYLVLIALVCLGVYLTLDWLL
Outside 221-237 GKDVAALAEQISQLFNA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9519 - -


  Protein sequence: 238 a.a.    

>T6CP000775 NC_007005:5881461-5882174 [Pseudomonas syringae pv. syringae B728a] [TssL]
MTEAVMQGAATAATEKPTLKDLVRDFITMALIVRRGRQAISVTAFESSVDTFFANLERQARSANYSVEQVKDAQYALCAF
LDESVLRSGDNDMRRHFEMEPLQFRYFGVHLAGEGFFEKIELLRADVKKNLDVLEVYHLCLALGFEGKFGLGQKDQLRYL
ANTLGQDIARYRKTPKALSPDWALPDQVSQMLRHEVPVWLYLVLIALVCLGVYLTLDWLLGKDVAALAEQISQLFNA*

  Nucleotide sequence: 714 bp    

>T6CP000775 NC_007005:5881461-5882174 [Pseudomonas syringae pv. syringae B728a] [TssL]
ATGACTGAAGCCGTGATGCAAGGTGCCGCTACGGCCGCAACCGAAAAACCTACGCTCAAAGACCTGGTGCGTGACTTCAT
CACCATGGCGCTGATTGTCCGTCGCGGCCGTCAGGCGATCTCGGTCACCGCCTTCGAATCCAGCGTCGATACTTTTTTCG
CCAACCTGGAGCGTCAGGCGCGCAGCGCCAACTACAGCGTCGAACAGGTCAAGGACGCCCAATACGCACTGTGCGCATTC
CTCGATGAGAGCGTGCTGCGTTCGGGCGACAACGACATGCGTCGCCACTTCGAAATGGAGCCGTTGCAGTTCCGTTATTT
CGGGGTTCACCTGGCGGGCGAAGGCTTCTTCGAGAAAATCGAACTGCTGCGCGCCGACGTGAAAAAGAACCTGGATGTGC
TCGAGGTGTATCACCTGTGCCTGGCCCTGGGTTTCGAAGGCAAATTCGGGCTGGGTCAGAAGGACCAGTTGCGCTACCTG
GCCAACACGCTGGGCCAGGACATCGCACGCTACCGCAAGACGCCAAAGGCGCTGTCGCCTGACTGGGCATTGCCTGACCA
GGTGTCGCAAATGCTGCGCCATGAAGTTCCGGTATGGCTTTACCTGGTGCTCATCGCCCTCGTATGCCTGGGTGTCTATC
TGACGCTTGACTGGTTGCTGGGCAAAGACGTGGCCGCGTTGGCCGAACAAATCAGCCAACTGTTCAATGCTTGA