Detailed information of regulator


Summary

Regulator ID REG0152 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (930 a.a.); Nucleotide sequence (2790 bp)
Description Results of the qRT-PCR studies of the B728a ΔretS strain exhibited a 2.8-fold increase in icmF transcript levels compared to those of wild-type B728a. The secretion of Hcp-VSV by the B728a ΔretS strain was reduced to nearly wild-type levels (2.5% of total Hcp-VSV in the culture) when intact retS was present in trans.
Reference
External database links

Locus tag (Gene) Psyr_4408 (retS)
Coordinates (Strand) 5270882..5273671 (+)
NCBI ID 489494774
RefSeq NC_007005
Uniprot ID Q4ZN34_PSEU2, A0A0Q0AN12_PSESX, A0A132FMG9_PSESY
KEGG ID psb:Psyr_4408
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
T6SS transcription↑-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
Response_regPF00072.264e-22792..906
Response_regPF00072.263.4e-19643..759
HisKAPF00512.275.4e-18397..461
7TMR-DISMED2PF07696.131.5e-1731..158
7TMR-DISM_7TMPF07695.132.2e-17172..372
HATPase_cPF02518.281.7e-13508..623
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Inside1-2VR
Transmembrane helix3-25WLRIAIASTVGLLTLLFMLSAQA
Outside26-168EHGAGWSTLLDEKAHLTLDEIRSARYQNQFSPIELERVTAADRDSALWLHYRLQPTQHEQLLRIFAPDLSSADMYVMDGDRQIDHVRTGNDVPIEDQRLPSNDFLLPVPQSSAPLDIYLRLVSAQKMRPSITLEPAIASAANE
Transmembrane helix169-186TQPLLFGLLFGALSMLIV
Inside187-198QNLTRYGHTRSR
Transmembrane helix199-221SNLWLAACESLLGLSALLLLNLL
Outside222-235RPIDPWNIAQTPSA
Transmembrane helix236-258HLALLLAAVAGLIYTYCFFVHRN
Inside259-264ARKLDR
Transmembrane helix265-282LLLGNAVLMGIGALLVLF
Outside283-285DEK
Transmembrane helix286-308LPINFLTFGLVSLTTLSILAVSM
Inside309-319WHWQKGYRSAR
Transmembrane helix320-342LFVLGMITFNIGYMVVLPGLLWL
Outside343-347SLVPP
Transmembrane helix348-370QWLILALLSVFCISGVLMSLALS
Inside371-929ERHRSITEDRFSISRDQAASTAEINAKAEFLAKISHEIRTPMNGVLGMTELLLGTPLSVKQRDYVQTIHSAGNELLTLINEILDITKLESGQIELDDVQFDIGALVEDCLNIFRAKAEQQQVELISQIQPQVPRVINGDPTRLRQTLLSLLENALKKTDEGEILLAVSLESSKSGNTRLRISVQDSGESLSDEEREELLHAELHSRNFLASSRLGGHLGLVIARQLIVLMDGEFGIQNTGAQGNTLWLNLPLDAKLLEQPTIDLDSPLKDARVLVVDDNDTCRKVLVQQCSAWGLNVSAVASGKEALALLRTKAHLRDYFDVVLLDQNMPGMTGMQLAAKIKEDPSLNHDILLIMLTGISNAPSKIIARNSGIKRILAKPVAGYTLKTTLADELTQLNKGVVSNKPGPVVTPPVNVPGDFRILVAEDNSISTKVIRGMLGKLNLNPDTASNGEEALRAMKAQRYDLVLMDCEMPILDGFSATEQLRAWEVGNQRVRTPVVALTAHILTEHKDRARQAGMDGHMAKPIELSQLRELVEHWVAHRDKARGLTSDGEHYRQN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
SP(Sec/SPI)0.9394pos: 25-26. AQA-EH.VRWLRIAIASTVGLLTLLFMLSAQA


  Protein sequence of regulator: 930 a.a.    .

>REG0152 NC_007005:5270882-5273671 [Pseudomonas syringae pv. syringae B728a]
VRWLRIAIASTVGLLTLLFMLSAQAEHGAGWSTLLDEKAHLTLDEIRSARYQNQFSPIELERVTAADRDSALWLHYRLQP
TQHEQLLRIFAPDLSSADMYVMDGDRQIDHVRTGNDVPIEDQRLPSNDFLLPVPQSSAPLDIYLRLVSAQKMRPSITLEP
AIASAANETQPLLFGLLFGALSMLIVQNLTRYGHTRSRSNLWLAACESLLGLSALLLLNLLRPIDPWNIAQTPSAHLALL
LAAVAGLIYTYCFFVHRNARKLDRLLLGNAVLMGIGALLVLFDEKLPINFLTFGLVSLTTLSILAVSMWHWQKGYRSARL
FVLGMITFNIGYMVVLPGLLWLSLVPPQWLILALLSVFCISGVLMSLALSERHRSITEDRFSISRDQAASTAEINAKAEF
LAKISHEIRTPMNGVLGMTELLLGTPLSVKQRDYVQTIHSAGNELLTLINEILDITKLESGQIELDDVQFDIGALVEDCL
NIFRAKAEQQQVELISQIQPQVPRVINGDPTRLRQTLLSLLENALKKTDEGEILLAVSLESSKSGNTRLRISVQDSGESL
SDEEREELLHAELHSRNFLASSRLGGHLGLVIARQLIVLMDGEFGIQNTGAQGNTLWLNLPLDAKLLEQPTIDLDSPLKD
ARVLVVDDNDTCRKVLVQQCSAWGLNVSAVASGKEALALLRTKAHLRDYFDVVLLDQNMPGMTGMQLAAKIKEDPSLNHD
ILLIMLTGISNAPSKIIARNSGIKRILAKPVAGYTLKTTLADELTQLNKGVVSNKPGPVVTPPVNVPGDFRILVAEDNSI
STKVIRGMLGKLNLNPDTASNGEEALRAMKAQRYDLVLMDCEMPILDGFSATEQLRAWEVGNQRVRTPVVALTAHILTEH
KDRARQAGMDGHMAKPIELSQLRELVEHWVAHRDKARGLTSDGEHYRQN*

  Nucleotide sequence of regulator: 2790 bp    .

>REG0152 NC_007005:5270882-5273671 [Pseudomonas syringae pv. syringae B728a]
GTGCGCTGGCTCAGGATTGCCATAGCCTCAACCGTCGGACTGCTGACTCTGCTGTTCATGCTTTCGGCACAGGCCGAGCA
TGGTGCAGGCTGGTCGACGCTGCTCGACGAAAAGGCCCACCTGACGCTGGATGAAATCCGCTCGGCGCGCTATCAGAATC
AATTCAGCCCCATCGAGCTGGAACGTGTCACGGCTGCGGATCGCGACAGCGCATTATGGCTGCACTACCGCCTGCAACCG
ACCCAGCACGAGCAATTGCTGCGCATCTTCGCCCCGGACCTGTCCAGCGCCGACATGTATGTCATGGACGGCGATCGACA
GATTGATCATGTACGCACGGGCAATGACGTACCGATTGAAGATCAGCGACTGCCGTCCAACGATTTCCTGCTGCCGGTTC
CGCAAAGCTCGGCCCCGCTGGATATCTACCTGAGGCTGGTCTCCGCGCAGAAAATGCGCCCCAGCATTACCCTTGAACCG
GCCATCGCAAGCGCCGCCAATGAGACTCAGCCATTGCTGTTCGGGCTGCTGTTTGGCGCACTGTCCATGCTGATCGTGCA
GAACCTGACGCGTTACGGGCACACCCGGTCGCGCAGCAATCTGTGGCTGGCCGCCTGCGAATCATTACTCGGGCTCAGTG
CCCTGTTGCTCCTCAACCTGCTGCGCCCGATCGACCCGTGGAATATCGCGCAAACCCCGAGCGCGCACCTGGCACTGTTG
CTGGCCGCTGTCGCAGGCCTGATCTACACCTACTGTTTCTTCGTCCACCGCAATGCCCGCAAGCTCGATCGCCTGTTGCT
GGGCAACGCAGTGCTCATGGGGATCGGCGCCCTGCTGGTGCTGTTCGACGAAAAGCTGCCGATCAATTTCCTGACGTTCG
GCCTGGTCTCGTTGACGACATTGAGCATTCTGGCGGTGTCGATGTGGCACTGGCAGAAGGGCTATCGCTCGGCACGCCTG
TTTGTCCTGGGGATGATCACCTTCAATATCGGCTACATGGTGGTCCTGCCCGGCCTGCTGTGGCTGAGCCTGGTGCCGCC
GCAATGGCTCATACTGGCGCTGCTCAGCGTGTTCTGCATCAGCGGTGTGCTGATGAGCCTGGCGCTCAGCGAACGCCATC
GCAGCATCACCGAAGACCGTTTCAGCATCAGCCGCGATCAGGCGGCCAGCACCGCCGAAATCAACGCCAAAGCCGAGTTT
CTGGCCAAGATCAGCCACGAGATTCGCACCCCGATGAATGGCGTGCTGGGCATGACCGAGCTGCTGCTCGGCACCCCGCT
GTCGGTCAAGCAGCGCGATTACGTGCAGACCATCCACAGTGCCGGTAACGAACTGCTGACGCTGATCAACGAGATTCTCG
ACATCACCAAGCTCGAGTCAGGGCAGATCGAACTGGACGACGTGCAGTTCGACATCGGCGCGCTGGTCGAGGACTGCCTG
AATATCTTCCGCGCCAAGGCCGAGCAGCAACAGGTCGAACTGATCAGCCAGATCCAGCCGCAGGTGCCACGTGTCATCAA
TGGTGATCCGACTCGCCTGCGCCAGACATTGCTGAGCCTGCTGGAAAATGCCCTGAAGAAGACCGACGAAGGGGAAATTC
TGCTGGCCGTATCCCTTGAGTCATCGAAAAGCGGCAATACGCGGCTGCGTATTTCGGTGCAGGACAGCGGCGAATCGCTT
TCCGACGAAGAGCGTGAAGAGCTGCTGCACGCCGAACTGCACAGCCGTAACTTCCTGGCCAGCAGCCGGCTCGGCGGCCA
CCTCGGGCTGGTGATCGCTCGTCAGTTGATCGTACTGATGGATGGCGAGTTCGGCATTCAGAACACGGGTGCGCAAGGTA
ATACGCTGTGGCTGAACCTGCCGCTGGACGCAAAGCTGCTTGAACAGCCGACCATCGATCTGGACAGCCCGCTCAAAGAC
GCCAGGGTGCTGGTGGTCGACGACAACGACACGTGCCGCAAAGTGCTGGTCCAGCAATGCAGCGCCTGGGGCCTGAATGT
CAGCGCCGTGGCGTCCGGCAAGGAGGCACTGGCCCTGCTCAGAACCAAGGCGCACCTGCGCGACTATTTCGATGTGGTCC
TGCTCGATCAGAACATGCCAGGCATGACCGGCATGCAACTGGCCGCCAAGATCAAGGAAGATCCAAGCCTGAATCACGAC
ATTCTGTTGATCATGCTGACCGGCATCAGCAATGCGCCCAGCAAGATCATCGCGCGCAACTCCGGCATCAAGCGCATTCT
GGCCAAACCGGTGGCAGGCTACACGCTCAAGACCACGCTGGCCGATGAACTGACTCAGTTGAACAAGGGTGTCGTGTCGA
ACAAGCCTGGCCCGGTCGTGACTCCGCCGGTCAACGTCCCTGGCGACTTCCGGATTCTGGTGGCCGAAGACAACAGCATT
TCGACCAAGGTCATCCGCGGCATGCTCGGCAAGCTCAACCTCAACCCCGATACCGCGAGTAATGGCGAAGAAGCGTTACG
TGCCATGAAGGCGCAGCGTTATGACCTGGTGTTGATGGACTGCGAGATGCCGATACTCGACGGCTTCTCGGCCACCGAGC
AATTGCGCGCCTGGGAAGTCGGCAATCAGCGTGTACGTACCCCGGTCGTTGCACTGACCGCGCATATTCTCACCGAGCAC
AAGGACCGCGCGCGCCAGGCCGGCATGGACGGCCACATGGCCAAGCCCATCGAATTGTCCCAGTTGCGCGAACTGGTCGA
GCACTGGGTGGCGCACCGTGACAAGGCCCGCGGGCTGACCTCGGACGGCGAGCACTATCGGCAAAACTGA