Detailed information of component protein


Summary

Component ID T6CP000774
Component protein type TssK
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (448 a.a.); Nucleotide sequence (1344 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25755
Coordinate (Strand) 5880121..5881464 (+)
NCBI ID WP_003343637.1
RefSeq NC_007005
Uniprot ID A0A0P9GBF9_PSESX, A0A0P9SWE9_PSESX, Q4ZLI5_PSEU2, A0A326SR37_9PSED, A0A2V3Q9I7_9PSED, A0A7Z6UTT4_PSESH, A0A2S4J4B0_PSESY, A0A2V4PFQ9_PSESJ, A0A3M5WRQ5_9PSED, A0A3M5WN99_PSEAP
KEGG ID psb:Psyr_4960
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 6.6e-132 19..445
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-447 MSKQSRVMWSEGMFLLPQHFQYQDEFHQHQLAESTLRSTPFHWGVQTLEVDVEALATGSLQLKRLKLVFPDGSLYDAPQHDPLPAARDLKNLLKGGEIKVYAALKLPEPFGLNYVEDGQEQKSARRFRKQFDTLPDLNEGDLENEITSLRLNVVMLVDGDSLDGYSYCPIARLARNSIGGFNLDAHFVHPTLHVGTHETLVGLGRRLISVLQAKSKALSGRRRERADQIAEFGSSDVTLFWLLNTVNRAYPQLAHLLAHPRLHPERLYLFLAELAGGLLTFSLDTQLTDIPDYDHQDPAASLVKLDELVRLLLENVIPNQCIVINLSQVRPSYWQGQLLDPRLTEADFYISVHADMPGSSLLELVPRAFKVGSPEDIEVVVNSAMPGVTLNHSTRLPNAIPVRLDNHYFSIEPHGRVYERMMEAQAISFYAPSAFTNLKLELLAVLK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9936 - -


  Protein sequence: 448 a.a.    

>T6CP000774 NC_007005:5880121-5881464 [Pseudomonas syringae pv. syringae B728a] [TssK]
MSKQSRVMWSEGMFLLPQHFQYQDEFHQHQLAESTLRSTPFHWGVQTLEVDVEALATGSLQLKRLKLVFPDGSLYDAPQH
DPLPAARDLKNLLKGGEIKVYAALKLPEPFGLNYVEDGQEQKSARRFRKQFDTLPDLNEGDLENEITSLRLNVVMLVDGD
SLDGYSYCPIARLARNSIGGFNLDAHFVHPTLHVGTHETLVGLGRRLISVLQAKSKALSGRRRERADQIAEFGSSDVTLF
WLLNTVNRAYPQLAHLLAHPRLHPERLYLFLAELAGGLLTFSLDTQLTDIPDYDHQDPAASLVKLDELVRLLLENVIPNQ
CIVINLSQVRPSYWQGQLLDPRLTEADFYISVHADMPGSSLLELVPRAFKVGSPEDIEVVVNSAMPGVTLNHSTRLPNAI
PVRLDNHYFSIEPHGRVYERMMEAQAISFYAPSAFTNLKLELLAVLK*

  Nucleotide sequence: 1344 bp    

>T6CP000774 NC_007005:5880121-5881464 [Pseudomonas syringae pv. syringae B728a] [TssK]
ATGAGTAAACAAAGCCGCGTGATGTGGTCGGAAGGCATGTTCCTTTTGCCGCAACATTTCCAATATCAGGATGAGTTTCA
TCAACATCAGCTTGCTGAGTCGACGCTGCGCTCCACGCCCTTTCACTGGGGCGTGCAAACGCTGGAAGTCGACGTCGAGG
CGCTGGCGACAGGCTCCCTGCAACTGAAGCGTCTGAAGCTGGTGTTCCCGGACGGCAGCCTTTACGACGCGCCGCAGCAC
GACCCGCTGCCCGCCGCCCGCGACCTGAAAAACCTGCTCAAAGGCGGAGAAATCAAGGTCTACGCGGCGCTCAAACTGCC
TGAGCCCTTTGGCCTGAATTACGTTGAAGACGGCCAGGAACAGAAGAGCGCGCGACGTTTTCGCAAACAGTTCGACACCC
TGCCGGACCTCAACGAAGGCGATCTGGAAAACGAAATCACCAGCCTGCGCCTGAACGTGGTGATGCTGGTCGATGGCGAC
AGTCTGGATGGCTACAGCTATTGCCCGATTGCCCGACTGGCACGCAACAGCATTGGCGGCTTCAACCTGGATGCGCATTT
TGTGCACCCGACGCTGCACGTGGGCACCCACGAGACGCTGGTCGGCCTCGGCAGACGTTTGATCAGCGTGCTGCAAGCCA
AGAGCAAGGCGCTTTCAGGCCGCCGCCGCGAGCGCGCTGACCAGATCGCCGAGTTCGGCTCCAGCGACGTCACCTTGTTC
TGGCTGCTGAATACCGTCAACCGCGCCTACCCGCAACTGGCTCACCTGCTGGCGCATCCACGCCTGCACCCGGAGCGCCT
GTATCTGTTTCTGGCGGAACTGGCGGGTGGTTTGCTGACCTTCTCGCTGGACACCCAGCTCACCGACATCCCCGATTACG
ATCATCAGGACCCGGCGGCCTCGTTGGTCAAGCTCGACGAACTGGTTCGTCTGCTGCTTGAAAACGTGATTCCGAACCAG
TGCATCGTCATCAACCTGAGTCAGGTCCGGCCTTCGTACTGGCAGGGCCAGTTGCTGGACCCGCGTCTTACCGAAGCCGA
CTTTTACATCTCGGTGCATGCCGACATGCCCGGCTCCAGCCTGCTGGAACTGGTGCCGCGCGCCTTCAAGGTCGGTTCGC
CGGAAGACATCGAAGTGGTGGTCAACAGCGCCATGCCGGGCGTCACGCTGAACCACTCGACGCGCCTGCCAAACGCCATT
CCGGTGCGCCTGGACAACCATTACTTCTCCATCGAGCCGCATGGCCGCGTGTACGAACGCATGATGGAAGCCCAGGCCAT
TTCCTTCTACGCGCCCAGCGCGTTTACCAATCTCAAGCTCGAACTGTTGGCGGTGCTTAAATGA