Detailed information of component protein


Summary

Component ID T6CP000781
Component protein type TssI
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25870
Coordinate (Strand) 5908301..5910145 (+)
NCBI ID WP_011269350.1
RefSeq NC_007005
Uniprot ID Q4ZLG2_PSEU2, A0A2V4Q8U9_PSESY
KEGG ID psb:Psyr_4983
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.8e-90 36..343
Phage_base_V PF04717.14 1.2e-15 394..462
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-614 MDLSSLFTAQNRRLFKLTMALKGGQELLLDSFAGSEGLSADFGFQLDLISDDASVKLKSAIGQSATIEIELATGGSRYINGHVLSFGNNGSDGGMSRYSAYIGSWLSLIAQRYDSQIYQDQTVEQVIRKVLGRYDGLAKYEFRVNRTLKPYSYITQYRESDSHFVMRLLEQEGLFFYFEHTADSHTLIIMDDSTVLTPLPEQPQVRFHTASVTETSDSITHWSSAREFQAGKISVQTFDYRQPGNRIPVTMNSLNQQGDVPSYEIFDYMGQYTHGTPEDGELLVRNRIEALELKGKTFVGSSNCRAMRPGYTFELTQHYEHDTGSPDDRQFLLMNIEHRGSNNYTSGSQAGYANKIYCVRKKIKFRPQMDLLKPGISGPQTAIVVGPPGEEIYTDEMGRVKVQFHWDRYGKFDDKSSCWVRVAQSGASGGFGSMQIPRVGDEVVVVFLDGNPDRPLVMGSLYNSQNTPPWSLPANKTQSGFLTRSAKGDGGTANFFRFEDKAGAEQVIMHAERNMDTEIELDEKHEVGNNRKVTVGGTNTEIIQKDTVTNVQQGSFTLKVDNQFIQVDAKQYILLKVGDSSISITPEGIQIKGKVINVLGESTFVKGDRVDINK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9049 - -


  Protein sequence: 615 a.a.    

>T6CP000781 NC_007005:5908301-5910145 [Pseudomonas syringae pv. syringae B728a] [TssI]
MDLSSLFTAQNRRLFKLTMALKGGQELLLDSFAGSEGLSADFGFQLDLISDDASVKLKSAIGQSATIEIELATGGSRYIN
GHVLSFGNNGSDGGMSRYSAYIGSWLSLIAQRYDSQIYQDQTVEQVIRKVLGRYDGLAKYEFRVNRTLKPYSYITQYRES
DSHFVMRLLEQEGLFFYFEHTADSHTLIIMDDSTVLTPLPEQPQVRFHTASVTETSDSITHWSSAREFQAGKISVQTFDY
RQPGNRIPVTMNSLNQQGDVPSYEIFDYMGQYTHGTPEDGELLVRNRIEALELKGKTFVGSSNCRAMRPGYTFELTQHYE
HDTGSPDDRQFLLMNIEHRGSNNYTSGSQAGYANKIYCVRKKIKFRPQMDLLKPGISGPQTAIVVGPPGEEIYTDEMGRV
KVQFHWDRYGKFDDKSSCWVRVAQSGASGGFGSMQIPRVGDEVVVVFLDGNPDRPLVMGSLYNSQNTPPWSLPANKTQSG
FLTRSAKGDGGTANFFRFEDKAGAEQVIMHAERNMDTEIELDEKHEVGNNRKVTVGGTNTEIIQKDTVTNVQQGSFTLKV
DNQFIQVDAKQYILLKVGDSSISITPEGIQIKGKVINVLGESTFVKGDRVDINK*

  Nucleotide sequence: 1845 bp    

>T6CP000781 NC_007005:5908301-5910145 [Pseudomonas syringae pv. syringae B728a] [TssI]
ATGGATCTATCCTCACTGTTTACGGCGCAGAACAGACGACTGTTCAAGCTGACGATGGCCCTCAAGGGTGGCCAGGAGCT
GTTGCTTGATTCCTTTGCCGGTAGTGAAGGTTTATCGGCTGATTTCGGGTTTCAACTCGATCTGATTTCTGATGATGCGA
GCGTAAAGCTTAAGTCCGCTATAGGGCAGTCCGCGACCATCGAAATCGAACTCGCCACCGGAGGCAGCCGTTACATTAAC
GGTCATGTATTGAGCTTCGGTAACAATGGCAGCGACGGTGGCATGTCGCGCTATTCAGCCTACATAGGCTCGTGGTTGAG
CCTGATTGCGCAGAGGTACGATTCTCAGATCTATCAGGATCAGACAGTGGAGCAGGTGATCCGGAAGGTATTGGGTCGCT
ACGATGGGCTTGCGAAGTACGAGTTCCGTGTCAATCGCACGCTCAAGCCATACAGCTACATCACCCAATACCGCGAAAGC
GATAGTCACTTCGTCATGCGCCTGCTGGAGCAGGAGGGTTTGTTCTTCTACTTTGAGCATACGGCGGACAGCCATACGCT
GATCATCATGGACGACTCGACTGTACTCACACCGCTTCCAGAGCAGCCCCAGGTTCGCTTCCATACAGCTTCTGTAACCG
AAACCTCTGACTCCATCACCCACTGGAGTTCAGCCCGGGAATTCCAGGCTGGCAAGATTTCGGTACAAACCTTCGATTAC
AGGCAGCCAGGAAATCGTATTCCCGTCACGATGAACAGCCTTAATCAGCAAGGCGATGTTCCATCGTATGAAATCTTTGA
TTACATGGGGCAGTACACGCACGGCACGCCCGAAGATGGTGAGCTCCTCGTGCGTAATCGTATCGAGGCACTGGAGCTAA
AAGGTAAGACATTCGTAGGGTCGAGTAACTGTCGGGCGATGCGTCCAGGTTACACATTTGAACTGACGCAACATTACGAG
CATGACACGGGCTCTCCCGATGATCGGCAGTTCCTGTTAATGAATATCGAGCATCGCGGCAGCAATAATTACACCTCGGG
CAGTCAGGCGGGTTACGCCAATAAAATTTATTGCGTACGCAAAAAAATCAAGTTCCGGCCTCAGATGGATTTGCTCAAGC
CAGGCATCTCCGGCCCCCAAACCGCCATCGTCGTCGGCCCGCCCGGCGAAGAAATCTACACCGACGAAATGGGCCGGGTA
AAAGTGCAGTTCCACTGGGACCGCTACGGCAAGTTTGACGACAAAAGCTCCTGCTGGGTCCGCGTCGCTCAATCCGGCGC
AAGTGGCGGTTTTGGTAGCATGCAGATCCCGCGCGTCGGTGACGAGGTGGTGGTCGTTTTCCTGGATGGCAACCCTGACC
GTCCACTCGTCATGGGCAGTTTGTATAACAGCCAGAACACGCCGCCGTGGTCGCTGCCTGCCAACAAGACCCAGAGTGGT
TTCCTGACGCGTTCGGCCAAGGGGGATGGCGGTACGGCCAACTTCTTCCGCTTTGAAGATAAGGCCGGTGCCGAACAGGT
GATCATGCATGCCGAGCGCAACATGGACACAGAGATCGAGCTGGATGAAAAGCACGAGGTGGGCAATAACCGCAAGGTGA
CGGTCGGCGGCACGAATACGGAAATCATCCAGAAAGATACTGTGACGAACGTGCAGCAGGGGTCGTTTACGCTAAAGGTC
GACAACCAATTCATTCAGGTGGATGCCAAGCAGTACATCCTTCTCAAAGTGGGCGACAGCAGCATCTCGATCACTCCGGA
AGGCATTCAGATAAAAGGCAAAGTGATCAATGTTCTTGGCGAGAGCACCTTCGTCAAAGGTGACCGAGTCGACATCAACA
AGTGA