Detailed information of regulator


Summary

Regulator ID REG0149 experimental
Regulator type protein
Regulatory pathway (from literatures) (p)ppGpp regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Purine metabolism (map00230)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (702 a.a.); Nucleotide sequence (2106 bp)
Description 28 putative T6SS-associated genes of PstDC3000 and 25 T6SS-related genes of PssB728a were down-regulated in the (p)ppGpp0PstDC3000 and (p)ppGpp0PssB728a mutants, respectively.
Reference
External database links

Locus tag (Gene) PSYR_RS01070 (spoT)
Coordinates (Strand) 223004..225109 (-)
NCBI ID 489499092
RefSeq NC_007005
Uniprot ID A0A2V3QIG0_9PSED, A0A0P9MWM3_9PSED, A0A2S4HWZ9_PSESY
KEGG ID -
KEGG pathway (determined by BlastKOALA) map01100; map00230
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
T6SS transcripts↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
HD_4PF13328.81.2e-5026..175
RelA_SpoTPF04607.194.3e-36235..344
TGSPF02824.236.9e-18389..447
ACT_4PF13291.82.4e-15622..698
DUF5913PF19296.16.5e-09460..543
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-701MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9928--


  Protein sequence of regulator: 702 a.a.    .

>REG0149 NC_007005:c225109-223004 [Pseudomonas syringae pv. syringae B728a]
MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGI
AKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
ETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHL
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEI
QIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIME
LPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPEGPLAIR
GTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIA
LLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA*

  Nucleotide sequence of regulator: 2106 bp    .

>REG0149 NC_007005:c225109-223004 [Pseudomonas syringae pv. syringae B728a]
TTGCCGAGCATAGACGCCCTCGCCGACAGACTGTCGGCCTACCTCGGCAAGGACCAGGTCAATCTGGTCCGCCGAGCGTA
TTTCTACGCCGAACAAGCCCACGATGGCCAACGCCGTCGCAGCGGTGAAGCCTACGTCACCCATCCTCTCGCGGTGGCGA
ACATTCTTGCCGATATGCACATGGACCATCAGAGCCTGATGGCTGCGATGCTGCATGACGTCATCGAAGACACCGGGATT
GCCAAGGAAGCGCTCAGCGCCCAATTTGGCGAGACCGTCGCGGAACTGGTCGACGGGGTCAGCAAACTGACCCAGATGAA
TTTCGAGACCAAGGCCGAAGCCCAGGCGGAAAACTTCCAGAAAATGGCCATGGCCATGGCGCGCGACATTCGCGTCATCC
TGGTCAAGCTCGCTGATCGCCTGCACAACATGCGCACGCTGGAAGTCCTGTCCGGCGAAAAGCGTCGGCGCATCGCCAAG
GAAACCCTTGAAATCTATGCGCCCATCGCCAATCGGCTGGGCATGCACAGTGTGCGCATCGAATTCGAGGACCTGGGCTT
CAAGGCGATGTACCCGATGCGTTCCTCGCGAATCAACCAGGCGGTCAAGCGCGCCAGGGGCAATCGCAAGGAACTGGTCA
ACAAGATCGAAGAGTCCCTGAGTCATTGCCTGGCCGTCGATGGCATCGAAGGCGACGTCAGTGGGCGGCAGAAACATCTG
TATGGCATCTACAAGAAGATGCGCGGCAAGCGTCGCGCCTTCAACGAGATCATGGATGTCTACGCGTTCCGCATCATCGT
CGACAAGGTCGACACCTGCTATCGCGTGCTCGGCGCTGTACATAATCTGTACAAGCCGCTGCCCGGTCGCTTCAAGGATT
ACATCGCGATCCCCAAGGCCAACGGCTATCAGTCCCTGCACACGACATTGTTCGGCATGCATGGCGTGCCTATCGAGATC
CAGATTCGCACCCGCGAAATGGAAGAAATGGCCAACAACGGTATCGCGGCACACTGGCTGTACAAGTCCAGTGACGACGA
GCAGCCCAAGGGCACTCACGCCCGGGCCCGACAATGGGTCAAGGGCGTGCTGGAAATGCAGCAGCGCGCCGGCAACTCGC
TGGAATTCATCGAAAGCGTCAAGATCGACCTGTTCCCGGACGAGGTCTACGTTTTCACGCCCAAAGGCCGGATCATGGAG
CTGCCCAAAGGCTCCACAGCGGTGGATTTCGCCTACGCGGTACACACCGATGTCGGCAACAGCTGCATTGCCTGCCGGAT
CAACCGCCGGCTGGCGCCGCTTTCCGAACCGCTGCAAAGTGGCTCGACCGTCGAGATCGTCAGCGCTCCAGGCGCACGCC
CGAACCCGGCCTGGCTGAACTTTGTCGTCACCGGCAAGGCGCGTACCCACATTCGTCACGCACTCAAGCTGCAACGCCGT
TCCGAGTCGATCAATCTGGGCGAGCGCCTGCTGAACAAGGTCCTCAACGGGTTTGAGTGCAGCCTCGACAAGATCGGTCA
GGAGCGCATTCAACTGGTGCTCAACGAATACCGGGTCGAAGTGCTGGAAGACCTGCTCGAAGACATCGGCCTGGGTAATC
GCATGGCTTATGTCGTTGCCCGCCGCCTGCTGGGCGAAGGCGATCAGTTGCCAAGCCCTGAAGGCCCGCTGGCGATTCGT
GGCACCGAAGGCCTGGTGCTCAGCTACGCGAAATGCTGCACGCCAATCCCGGGCGACCCGATTGTCGGCCACCTGTCTGC
AGGCAAGGGCATGGTCGTGCACCTGGACAACTGCCGCAACATCAGCGAAATCCGCCACAACCCGGAAAAATGCATCCAGC
TGTCCTGGGCCAAGGACGTGACGGGCGAATTCAACGTCGAACTGCGCGTCGAACTCGAGCATCAGCGCGGCCTGATCGCG
CTGCTGGCCAGCAGCGTCAACGCGGCGGACGGCAATATCGAAAAAATCAGCATGGACGAACGCGATGGTCGCATCAGCGT
GGTCCAACTGGTGGTCAGCGTGCACGATCGCGTGCACCTGGCTCGTGTGATCAAGAAACTGCGCGCCCTGACCGGTGTCA
CACGCATCACCCGGATGCGCGCGTAG