Detailed information of regulator


Summary

Regulator ID REG0149 experimental
Regulator type protein
Regulatory pathway (from literatures) (p)ppGpp regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Purine metabolism (map00230)
Related T6SS T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (702 a.a.); Nucleotide sequence (2106 bp)
Description 28 putative T6SS-associated genes of PstDC3000 and 25 T6SS-related genes of PssB728a were down-regulated in the (p)ppGpp0PstDC3000 and (p)ppGpp0PssB728a mutants, respectively.
Reference

External database links

Locus tag (Gene) PSYR_RS01070 (spoT)
Coordinates (Strand) 223004..225109 (-)
NCBI ID 489499092
RefSeq NC_007005
Uniprot ID A0A0P9MWM3_9PSED, A0A2S4HWZ9_PSESY
KEGG ID -
KEGG pathway (determined by BlastKOALA) map01100; map00230
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HD_4 PF13328.8 1.2e-50 26..175
RelA_SpoT PF04607.19 4.3e-36 235..344
TGS PF02824.23 6.9e-18 389..447
ACT_4 PF13291.8 2.4e-15 622..698
DUF5913 PF19296.1 6.5e-09 460..543

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-701 MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9928 - -



  Protein sequence of regulator: 702 a.a.    .

>REG0149 NC_007005:c225109-223004 [Pseudomonas syringae pv. syringae B728a]
MPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGI
AKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
ETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSGRQKHL
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEI
QIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIME
LPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPEGPLAIR
GTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIA
LLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA*

  Nucleotide sequence of regulator: 2106 bp    .

>REG0149 NC_007005:c225109-223004 [Pseudomonas syringae pv. syringae B728a]
TTGCCGAGCATAGACGCCCTCGCCGACAGACTGTCGGCCTACCTCGGCAAGGACCAGGTCAATCTGGTCCGCCGAGCGTA
TTTCTACGCCGAACAAGCCCACGATGGCCAACGCCGTCGCAGCGGTGAAGCCTACGTCACCCATCCTCTCGCGGTGGCGA
ACATTCTTGCCGATATGCACATGGACCATCAGAGCCTGATGGCTGCGATGCTGCATGACGTCATCGAAGACACCGGGATT
GCCAAGGAAGCGCTCAGCGCCCAATTTGGCGAGACCGTCGCGGAACTGGTCGACGGGGTCAGCAAACTGACCCAGATGAA
TTTCGAGACCAAGGCCGAAGCCCAGGCGGAAAACTTCCAGAAAATGGCCATGGCCATGGCGCGCGACATTCGCGTCATCC
TGGTCAAGCTCGCTGATCGCCTGCACAACATGCGCACGCTGGAAGTCCTGTCCGGCGAAAAGCGTCGGCGCATCGCCAAG
GAAACCCTTGAAATCTATGCGCCCATCGCCAATCGGCTGGGCATGCACAGTGTGCGCATCGAATTCGAGGACCTGGGCTT
CAAGGCGATGTACCCGATGCGTTCCTCGCGAATCAACCAGGCGGTCAAGCGCGCCAGGGGCAATCGCAAGGAACTGGTCA
ACAAGATCGAAGAGTCCCTGAGTCATTGCCTGGCCGTCGATGGCATCGAAGGCGACGTCAGTGGGCGGCAGAAACATCTG
TATGGCATCTACAAGAAGATGCGCGGCAAGCGTCGCGCCTTCAACGAGATCATGGATGTCTACGCGTTCCGCATCATCGT
CGACAAGGTCGACACCTGCTATCGCGTGCTCGGCGCTGTACATAATCTGTACAAGCCGCTGCCCGGTCGCTTCAAGGATT
ACATCGCGATCCCCAAGGCCAACGGCTATCAGTCCCTGCACACGACATTGTTCGGCATGCATGGCGTGCCTATCGAGATC
CAGATTCGCACCCGCGAAATGGAAGAAATGGCCAACAACGGTATCGCGGCACACTGGCTGTACAAGTCCAGTGACGACGA
GCAGCCCAAGGGCACTCACGCCCGGGCCCGACAATGGGTCAAGGGCGTGCTGGAAATGCAGCAGCGCGCCGGCAACTCGC
TGGAATTCATCGAAAGCGTCAAGATCGACCTGTTCCCGGACGAGGTCTACGTTTTCACGCCCAAAGGCCGGATCATGGAG
CTGCCCAAAGGCTCCACAGCGGTGGATTTCGCCTACGCGGTACACACCGATGTCGGCAACAGCTGCATTGCCTGCCGGAT
CAACCGCCGGCTGGCGCCGCTTTCCGAACCGCTGCAAAGTGGCTCGACCGTCGAGATCGTCAGCGCTCCAGGCGCACGCC
CGAACCCGGCCTGGCTGAACTTTGTCGTCACCGGCAAGGCGCGTACCCACATTCGTCACGCACTCAAGCTGCAACGCCGT
TCCGAGTCGATCAATCTGGGCGAGCGCCTGCTGAACAAGGTCCTCAACGGGTTTGAGTGCAGCCTCGACAAGATCGGTCA
GGAGCGCATTCAACTGGTGCTCAACGAATACCGGGTCGAAGTGCTGGAAGACCTGCTCGAAGACATCGGCCTGGGTAATC
GCATGGCTTATGTCGTTGCCCGCCGCCTGCTGGGCGAAGGCGATCAGTTGCCAAGCCCTGAAGGCCCGCTGGCGATTCGT
GGCACCGAAGGCCTGGTGCTCAGCTACGCGAAATGCTGCACGCCAATCCCGGGCGACCCGATTGTCGGCCACCTGTCTGC
AGGCAAGGGCATGGTCGTGCACCTGGACAACTGCCGCAACATCAGCGAAATCCGCCACAACCCGGAAAAATGCATCCAGC
TGTCCTGGGCCAAGGACGTGACGGGCGAATTCAACGTCGAACTGCGCGTCGAACTCGAGCATCAGCGCGGCCTGATCGCG
CTGCTGGCCAGCAGCGTCAACGCGGCGGACGGCAATATCGAAAAAATCAGCATGGACGAACGCGATGGTCGCATCAGCGT
GGTCCAACTGGTGGTCAGCGTGCACGATCGCGTGCACCTGGCTCGTGTGATCAAGAAACTGCGCGCCCTGACCGGTGTCA
CACGCATCACCCGGATGCGCGCGTAG