Detailed information of component protein


Summary

Component ID T6CP000779
Component protein type TssA
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (365 a.a.); Nucleotide sequence (1095 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25785
Coordinate (Strand) 5890348..5891442 (+)
NCBI ID WP_003343647.1
RefSeq NC_007005
Uniprot ID A0A0P9IX19_PSESX, A0A3M2TU88_PSESX, Q4ZLH9_PSEU2, A0A326SRM5_9PSED, A0A2V3QFB9_9PSED, A0A3M4XZI2_9PSED, A0A2G9WBF6_PSESX, A0A7Z6UTX6_PSESH, A0A2S4J442_PSESY, A0A2V4PFY5_PSESJ, A0A0Q0C4P3_PSEAP
KEGG ID psb:Psyr_4966
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 1.8e-27 18..133
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-364 MSEFLNDLSLADLTAPINGGSGEDLSFSTLFDQVKEARRADPDYLTQGDWQTDLKSSDWEQTITLAAQGLAEQSKDLMLVAWLSEALAHKYHFVGITFGLTVAERILQNFWDDLYPSLEDGVEERAARLAWLKTTLTEVVGGLPITQGQNFGLLRYDESRHVENLALQNPKAMQSAVEEGKINAEIFQRSVVLTDSDHLRLKASEIAASLYACQQLQATADTLFGRDAPSLVTLSDILQRAGQLTEKLLKDRGIELSPAPAAAQAAPETVVDQPAGAVMTQASQDSASAPLRTTPTTRDEAFTMLAGIAQFFKNTEPQSPVPYLIERAIKWGNMPLEGWLNDVIKDSNVVDSIRDVLGTKEPKQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9900 - -


  Protein sequence: 365 a.a.    

>T6CP000779 NC_007005:5890348-5891442 [Pseudomonas syringae pv. syringae B728a] [TssA]
MSEFLNDLSLADLTAPINGGSGEDLSFSTLFDQVKEARRADPDYLTQGDWQTDLKSSDWEQTITLAAQGLAEQSKDLMLV
AWLSEALAHKYHFVGITFGLTVAERILQNFWDDLYPSLEDGVEERAARLAWLKTTLTEVVGGLPITQGQNFGLLRYDESR
HVENLALQNPKAMQSAVEEGKINAEIFQRSVVLTDSDHLRLKASEIAASLYACQQLQATADTLFGRDAPSLVTLSDILQR
AGQLTEKLLKDRGIELSPAPAAAQAAPETVVDQPAGAVMTQASQDSASAPLRTTPTTRDEAFTMLAGIAQFFKNTEPQSP
VPYLIERAIKWGNMPLEGWLNDVIKDSNVVDSIRDVLGTKEPKQ*

  Nucleotide sequence: 1095 bp    

>T6CP000779 NC_007005:5890348-5891442 [Pseudomonas syringae pv. syringae B728a] [TssA]
ATGTCTGAATTTCTCAACGACCTTTCGCTTGCCGATCTGACGGCACCCATCAACGGGGGAAGTGGCGAGGACCTGAGCTT
TTCCACCCTGTTCGATCAGGTCAAGGAAGCTCGTCGAGCAGACCCCGACTACCTCACCCAAGGTGACTGGCAAACCGATC
TCAAGTCCTCGGACTGGGAACAGACCATCACCTTGGCCGCACAGGGCCTTGCCGAACAAAGCAAGGACCTGATGCTCGTC
GCCTGGCTCAGCGAAGCCCTGGCGCACAAATACCACTTTGTCGGCATCACCTTCGGCCTGACCGTGGCCGAGCGGATCCT
GCAAAACTTCTGGGACGATCTGTACCCATCTCTGGAAGATGGCGTAGAAGAACGTGCCGCCCGCCTGGCCTGGCTGAAAA
CCACGCTGACCGAAGTCGTCGGCGGGCTGCCGATCACGCAGGGGCAGAATTTTGGCCTGTTGCGCTACGACGAGTCGCGC
CACGTTGAAAACCTCGCGCTGCAAAACCCCAAGGCCATGCAGTCCGCTGTTGAAGAAGGCAAGATCAACGCCGAAATCTT
CCAGCGCTCGGTGGTCCTGACCGACTCGGATCATCTGCGCCTCAAGGCCAGCGAAATCGCCGCCAGCCTGTACGCCTGCC
AGCAGCTCCAGGCCACTGCCGACACACTGTTCGGCCGCGACGCGCCGAGCCTTGTCACCCTCAGCGACATACTGCAACGC
GCCGGGCAACTGACCGAAAAGCTGCTCAAGGACCGAGGTATCGAACTGAGCCCCGCCCCCGCCGCCGCTCAGGCCGCACC
CGAAACCGTCGTCGACCAACCCGCAGGTGCCGTAATGACCCAGGCCAGCCAGGACAGCGCGTCCGCACCACTGCGCACCA
CCCCGACCACACGCGACGAAGCCTTCACCATGCTCGCCGGCATCGCACAGTTCTTCAAAAACACCGAGCCGCAAAGCCCC
GTCCCGTACCTCATCGAGCGCGCCATCAAGTGGGGCAACATGCCGCTGGAAGGCTGGCTGAACGACGTCATCAAGGACAG
CAACGTCGTGGACAGCATTCGCGATGTGCTGGGTACGAAAGAGCCTAAGCAGTAA