Detailed information of component protein


Summary

Component ID T6CP000771
Component protein type TssG
T6SS ID (Type) T6SS00086 experimental (Type i4b)
Strain Pseudomonas syringae pv. syringae B728a
Replicon chromosome
Sequence Protein sequence (357 a.a.); Nucleotide sequence (1071 bp)
Reference
[1] Records AR, Gross DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. J Bacteriol. 2010 Jul;192(14):3584-96. doi: 10.1128/JB.00114-10. Epub 2010 May 14. PMID: 20472799
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSYR_RS25740
Coordinate (Strand) 5875673..5876743 (+)
NCBI ID WP_011269329.1
RefSeq NC_007005
Uniprot ID Q4ZLI8_PSEU2, A0A2V4QT86_PSESY
KEGG ID psb:Psyr_4957
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssG PF06996.13 2.2e-103 19..333
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-356 MAPANRRSTPGLIDQARAEPHRFEFFQLVRLLRLHYSRNGRMDLETRPHEDPLRFRSQLSLNFPASEVSDLQFEREGKLSAAGLPLSEVQVTFMGLVGPSGVLPRPYTELLIDRHIQYRDDAAHAFLDIFSHRMTTLFYEAWQKYKFYIEYERNGTSNFDRYLLNLVGFGPEGLKQKFDKGESPLRRELFSYFSGMFAQKPRNALNLEVMLSFYFSLPFKIQQFAGRWLKLDSSQCTQLGRKNAVLGQSAVAGNRVWDYQSCVRIEMGPLELADYQRFQPGTEDYQKLVELVRFYIGAELDFQIAPELKREAVPVARLGRQGNVSLGWLGWLKRPGVDVEPSRCAVFHIPFDGVSL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9836 - -


  Protein sequence: 357 a.a.    

>T6CP000771 NC_007005:5875673-5876743 [Pseudomonas syringae pv. syringae B728a] [TssG]
MAPANRRSTPGLIDQARAEPHRFEFFQLVRLLRLHYSRNGRMDLETRPHEDPLRFRSQLSLNFPASEVSDLQFEREGKLS
AAGLPLSEVQVTFMGLVGPSGVLPRPYTELLIDRHIQYRDDAAHAFLDIFSHRMTTLFYEAWQKYKFYIEYERNGTSNFD
RYLLNLVGFGPEGLKQKFDKGESPLRRELFSYFSGMFAQKPRNALNLEVMLSFYFSLPFKIQQFAGRWLKLDSSQCTQLG
RKNAVLGQSAVAGNRVWDYQSCVRIEMGPLELADYQRFQPGTEDYQKLVELVRFYIGAELDFQIAPELKREAVPVARLGR
QGNVSLGWLGWLKRPGVDVEPSRCAVFHIPFDGVSL*

  Nucleotide sequence: 1071 bp    

>T6CP000771 NC_007005:5875673-5876743 [Pseudomonas syringae pv. syringae B728a] [TssG]
ATGGCCCCCGCGAACAGGCGATCAACCCCTGGTCTGATCGACCAGGCAAGGGCTGAGCCGCACCGATTCGAATTTTTCCA
GTTGGTGCGTTTGCTTCGCCTGCACTACAGCAGGAACGGGCGTATGGACCTGGAAACCAGGCCGCACGAAGACCCTCTGC
GCTTTCGTTCGCAGCTGTCGCTGAACTTCCCGGCCAGTGAAGTCAGCGACCTGCAGTTCGAACGCGAAGGCAAGCTCTCG
GCGGCAGGTTTGCCGCTGTCCGAAGTGCAGGTCACGTTCATGGGCCTGGTCGGTCCTTCGGGTGTGCTGCCGCGCCCGTA
CACCGAGCTATTGATCGACCGACATATTCAGTATCGAGACGACGCTGCACATGCGTTTCTGGATATTTTTTCGCATCGCA
TGACCACGCTGTTTTACGAGGCGTGGCAGAAGTACAAGTTTTACATCGAGTACGAGCGCAACGGCACTTCGAACTTCGAC
CGTTATTTGCTCAACCTGGTGGGTTTCGGTCCAGAAGGCCTGAAGCAGAAGTTCGACAAGGGCGAGTCTCCCCTGCGCCG
CGAACTGTTCAGCTACTTCTCGGGGATGTTCGCGCAGAAGCCGCGCAACGCCCTGAACCTTGAGGTCATGCTGAGTTTTT
ACTTCTCGCTGCCGTTCAAGATCCAGCAGTTCGCCGGACGCTGGCTGAAGCTCGACAGCAGCCAGTGCACGCAGCTGGGC
CGCAAGAACGCCGTGCTGGGGCAAAGCGCCGTGGCCGGCAATCGCGTCTGGGATTACCAGTCGTGTGTACGTATCGAAAT
GGGTCCTCTGGAACTGGCCGACTACCAGCGCTTTCAGCCGGGCACCGAGGATTACCAGAAGCTTGTTGAACTGGTGCGTT
TTTACATCGGTGCCGAACTGGACTTCCAGATTGCGCCGGAGCTCAAGCGCGAAGCTGTACCCGTGGCCCGCCTGGGTCGC
CAAGGCAATGTATCGCTGGGCTGGCTTGGCTGGCTCAAACGCCCCGGTGTCGACGTGGAGCCTTCCCGTTGCGCCGTTTT
CCATATTCCTTTTGATGGGGTCTCCCTGTGA