Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00158 experimental
T6SS type Type ii
Strain Francisella tularensis subsp. novicida U112
Replicon (RefSeq) chromosome (NC_008601)
Location 1378294..1404786
Function anti-eukaryotic; effector translocation; virulence
Comment Intracellular growth in cultured murine macrophages.
References
[1] de Bruin OM et al (2011) The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]
[2] Robb CS et al (2010) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida. Acta Crystallogr Sect F Struct Biol Cryst Commun. 66(Pt 12):1596-8. [PudMed:21139203]
[3] Nano FE et al (2007) The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
[4] Broms JE et al (2012) Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]
[5] Barker JR et al (2009) The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol Microbiol. 74(6):1459-70. [PudMed:20054881]
[6] Hare RF et al (2014) Francisella novicida Pathogenicity Island Encoded Proteins Were Secreted during Infection of Macrophage-Like Cells. PLoS One. 9(8):e105773. [PudMed:25158041]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentClpBIglCIglDIglEIglFIglGIglHIglIIglJPdpAPdpBPdpCPdpDPdpETssBTssCTssITssL
Number111111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
T6CP004914 PdpA FTN_RS06705 1378294..1380756 + 2463 WP_003040109.1 hypothetical protein
T6CP004915 PdpB FTN_RS06710 1380761..1384042 + 3282 WP_003040112.1 hypothetical protein
T6CP004916 IglE FTN_RS06715 1384032..1384409 + 378 WP_003040115.1 hypothetical protein
T6CP004917 TssI FTN_RS06720 1384422..1384916 + 495 WP_003037116.1 hypothetical protein
T6CP004918 IglF FTN_RS06725 1384921..1386651 + 1731 WP_003040118.1 hypothetical protein
T6CP004919 IglG FTN_RS06730 1386652..1387173 + 522 WP_003040120.1 DUF4280 domain-containing protein
T6CP004920 IglH FTN_RS06735 1387186..1388616 + 1431 WP_003040123.1 hypothetical protein
T6CP004921 TssL FTN_RS06740 1388629..1389252 + 624 WP_003040125.1 DotU family type IV/VI secretion system protein
T6CP004922 IglI FTN_RS06745 1389274..1390425 + 1152 WP_003040128.1 hypothetical protein
T6CP004923 IglJ FTN_RS06750 1390391..1391164 + 774 WP_011733698.1 hypothetical protein
T6CP004924 PdpC FTN_RS06755 (pdpC) 1391204..1395181 + 3978 WP_003040134.1 pathogenicity determinant protein PdpC
T6CP004925 PdpE FTN_RS06760 1395199..1395765 + 567 WP_003040137.1 hypothetical protein
T6CP004926 IglD FTN_RS06765 1395772..1396968 - 1197 WP_003040140.1 hypothetical protein
T6CP004927 IglC FTN_RS06770 1396989..1397618 - 630 WP_003016165.1 hypothetical protein
T6CP004928 TssC FTN_RS06775 (tssC) 1397635..1399155 - 1521 WP_003040143.1 type VI secretion system contractile sheath large subunit
T6CP004929 TssB FTN_RS06780 (tssB) 1399169..1399723 - 555 WP_003040146.1 type VI secretion system contractile sheath small subunit
T6CP004930 PdpD FTN_RS06785 1399803..1403540 - 3738 WP_003040148.1 hypothetical protein
FTN_RS06790 1403641..1404786 - 1146 WP_011733699.1 anhydro-N-acetylmuramic acid kinase
FTN_RS06795 1404933..1406117 + 1185 pseudogene MFS transporter
FTN_RS06800 1406119..1407573 + 1455 WP_011733700.1 alpha,alpha-trehalase
FTN_RS06805 1407740..1408804 - 1065 WP_003034542.1 fructose-bisphosphate aldolase class II
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0173 experimental FTN_RS06610 (mglA) protein TCS regulatory pathway Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1362282..1362899 (+) 489124662 iglC transcription (↓); Unknown (↓)
REG0174 experimental FTN_RS02845 (SspA) protein TCS regulatory pathway Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 574840..575472 (+) 500053699 FTN_1465 (pmrA) (↓)
REG0175 experimental FTN_RS08805 (KdpD) protein TCS-HK Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1838601..1841282 (-) 489131611 FTN_1465 (pmrA) (↓)
REG0176 experimental FTN_1465 (pmrA) protein TCS-RR; TCS regulatory pathway Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1553358..1554044 (-) 489108239 FTN_1465 (pmrA) promotor (↓); pdpD promotor (↓); IglA expression (↓)
REG0177 experimental FTN_RS06615 (mglB) protein Global regulator; TCS regulatory pathway Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1362901..1363308 (+) 489112095 IglA expression (↓); T6SS Transciption (↓)
REG0178 experimental FTN_0480 (fevR/PigR) protein TCS regulatory pathwayre Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 486541..486876 (+) 489108070 T6SS Transciption (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01494 experimental FTN_1319 (pdpC) Tle - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1391204..1395181 (+) 118497899
EFF01487 experimental FTN_0131 (opiA) Tse - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 143587..144894 (-) 118496746
EFF01489 experimental FTN_1325 (pdpD) Tse - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1399803..1403540 (-) 118497905
EFF01491 experimental FTN_1069 (OpiB-1) Tse - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1128155..1130653 (-) 118497660
EFF01492 experimental FTN_1070 (OpiB-2) Tse - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1130861..1132213 (-) 118497661
EFF01493 experimental FTN_1071 (OpiB-3) Tse - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1132421..1133899 (-) 118497662
EFF00204 experimental FTN_1317 (IglI) - - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1389274..1390425 (+) 118497897
EFF00203 experimental FTN_1312 (VgrG) - - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1384422..1384916 (+) 118497892
EFF01488 experimental FTN_1309 (pdpA) - - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1378294..1380756 (+) 118497889
EFF01490 experimental FTN_1322 (iglC) - - Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1396989..1397618 (-) 118497902

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00023 experimental FTN_RS06580 (dnaK) chaperone Francisella tularensis subsp. novicida U112 chromosome (NC_008601) 1356202..1358130 (-) 489130276 FTN_RS08950 (ClpB) -