Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00158  |
| T6SS type | Type ii |
| Strain | Francisella tularensis subsp. novicida U112 |
| Replicon (RefSeq) | chromosome (NC_008601) |
| Location | 1378294..1404786 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Intracellular growth in cultured murine macrophages. |
| References | [1] Nano FE et al (2007) The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722] [2] Broms JE et al (2012) Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] [3] Barker JR et al (2009) The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol Microbiol. 74(6):1459-70. [PudMed:20054881] [4] Hare RF et al (2014) Francisella novicida Pathogenicity Island Encoded Proteins Were Secreted during Infection of Macrophage-Like Cells. PLoS One. 9(8):e105773. [PudMed:25158041] [5] de Bruin OM et al (2011) The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] [6] Robb CS et al (2010) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida. Acta Crystallogr Sect F Struct Biol Cryst Commun. 66(Pt 12):1596-8. [PudMed:21139203] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | ClpB | IglC | IglD | IglE | IglF | IglG | IglH | IglI | IglJ | PdpA | PdpB | PdpC | PdpD | PdpE | TssB | TssC | TssI | TssL |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| T6CP004914 | PdpA | FTN_RS06705 | 1378294..1380756 | + | 2463 | WP_003040109.1 | hypothetical protein |
| T6CP004915 | PdpB | FTN_RS06710 | 1380761..1384042 | + | 3282 | WP_003040112.1 | hypothetical protein |
| T6CP004916 | IglE | FTN_RS06715 | 1384032..1384409 | + | 378 | WP_003040115.1 | hypothetical protein |
| T6CP004917 | TssI | FTN_RS06720 | 1384422..1384916 | + | 495 | WP_003037116.1 | hypothetical protein |
| T6CP004918 | IglF | FTN_RS06725 | 1384921..1386651 | + | 1731 | WP_003040118.1 | hypothetical protein |
| T6CP004919 | IglG | FTN_RS06730 | 1386652..1387173 | + | 522 | WP_003040120.1 | DUF4280 domain-containing protein |
| T6CP004920 | IglH | FTN_RS06735 | 1387186..1388616 | + | 1431 | WP_003040123.1 | hypothetical protein |
| T6CP004921 | TssL | FTN_RS06740 | 1388629..1389252 | + | 624 | WP_003040125.1 | DotU family type IV/VI secretion system protein |
| T6CP004922 | IglI | FTN_RS06745 | 1389274..1390425 | + | 1152 | WP_003040128.1 | hypothetical protein |
| T6CP004923 | IglJ | FTN_RS06750 | 1390391..1391164 | + | 774 | WP_011733698.1 | hypothetical protein |
| T6CP004924 | PdpC | FTN_RS06755 (pdpC) | 1391204..1395181 | + | 3978 | WP_003040134.1 | pathogenicity determinant protein PdpC |
| T6CP004925 | PdpE | FTN_RS06760 | 1395199..1395765 | + | 567 | WP_003040137.1 | hypothetical protein |
| T6CP004926 | IglD | FTN_RS06765 | 1395772..1396968 | - | 1197 | WP_003040140.1 | hypothetical protein |
| T6CP004927 | IglC | FTN_RS06770 | 1396989..1397618 | - | 630 | WP_003016165.1 | hypothetical protein |
| T6CP004928 | TssC | FTN_RS06775 (tssC) | 1397635..1399155 | - | 1521 | WP_003040143.1 | type VI secretion system contractile sheath large subunit |
| T6CP004929 | TssB | FTN_RS06780 (tssB) | 1399169..1399723 | - | 555 | WP_003040146.1 | type VI secretion system contractile sheath small subunit |
| T6CP004930 | PdpD | FTN_RS06785 | 1399803..1403540 | - | 3738 | WP_003040148.1 | hypothetical protein |
| FTN_RS06790 | 1403641..1404786 | - | 1146 | WP_011733699.1 | anhydro-N-acetylmuramic acid kinase | ||
| FTN_RS06795 | 1404933..1406117 | + | 1185 | pseudogene | MFS transporter | ||
| FTN_RS06800 | 1406119..1407573 | + | 1455 | WP_011733700.1 | alpha,alpha-trehalase | ||
| FTN_RS06805 | 1407740..1408804 | - | 1065 | WP_003034542.1 | fructose-bisphosphate aldolase class II |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|---|---|---|---|---|---|---|---|
| ACP00023 |
FTN_RS06580 (dnaK) | chaperone | Francisella tularensis subsp. novicida U112 | chromosome (NC_008601) | 1356202..1358130 (-) | 489130276 | FTN_RS08950 (ClpB) | - |