Detailed information of component protein


Summary

Component ID T6CP004928
Component protein type TssC
T6SS ID (Type) T6SS00158 experimental (Type ii)
Strain Francisella tularensis subsp. novicida U112
Replicon chromosome
Sequence Protein sequence (507 a.a.); Nucleotide sequence (1521 bp)
Reference
[1] de Bruin OM, Duplantis BN, Ludu JS, et al. The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology (Reading). 2011 Dec;157(Pt 12):3483-3491. doi: 10.1099/mic.0.052308-0. Epub 2011 Oct 6. PMID: 21980115
[2] Robb CS, Nano FE, Boraston AB. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1596-8. doi: 10.1107/S1744309110034378. Epub 2010 Nov 25. PMID: 21139203
[3] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[4] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[5] Barker JR, Chong A, Wehrly TD, et al. The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol Microbiol. 2009 Dec;74(6):1459-70. PMID: 20054881
[6] Hare RF, Hueffer K. Francisella novicida pathogenicity island encoded proteins were secreted during infection of macrophage-like cells. PLoS One. 2014 Aug 26;9(8):e105773. doi: 10.1371/journal.pone.0105773. eCollection 2014. PMID: 25158041
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTN_RS06775
Coordinate (Strand) 1397635..1399155 (-)
NCBI ID WP_003040143.1
RefSeq NC_008601
Uniprot ID A0Q7I4_FRATN
KEGG ID ftn:FTN_1323, ftx:AW25_681
PDB ID 3J9O
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 1e-95 75..375
VipB_2 PF18945.2 6.3e-38 387..496
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-506 MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQKYIQKVITVIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKNESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSLANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPHIQFEGMNTTMTIDTRLEPELFGTNNN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9908 - -


  Protein sequence: 507 a.a.    

>T6CP004928 NC_008601:c1399155-1397635 [Francisella tularensis subsp. novicida U112] [TssC]
MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQKYIQKVITVI
DKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGG
EPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVA
AYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKNESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKN
LVACVYDNKGILETKSPLNVLFADYMELSLANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTM
CISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPH
IQFEGMNTTMTIDTRLEPELFGTNNN*

  Nucleotide sequence: 1521 bp    

>T6CP004928 NC_008601:c1399155-1397635 [Francisella tularensis subsp. novicida U112] [TssC]
ATGACAATAAATAAATTAAGTCTCACTGATGAACTTTTAAATAATTTTGGGGGATCTACAGAAGTTGATAGTGTACTCAA
AAATATAGATTTTGATGTTTCAGATGATGCTTCTAAAGTTTTATCTTTATCTACTGACTACAATGCTAGAAACCTTATGG
CGCTATCTTTGGTATTAGCAAATAATGATAATATAAATAATTACAATCAAAAATATATTCAAAAAGTTATTACAGTTATT
GATAAGCTGATTGATTTACAAGTTAACTCTATTATATCTAATGATGAGTTTAGAGCACTTGAGCAAGAATGGCTAAAGGT
GCAAGAGGTTTGTCAAGAAGACTATGATAATGTTGAAGTAAGTATATTAGATGTAAAAAAAGAAGAGCTACAATATGATT
TCGAGAGAAATTTATATGATATATCTAGTAGTGACTTTTTCAAAAAAGTATATGTTTCAGAATTTGATCAATATGGTGGC
GAACCTTATGGCGCAATATTAGGATTGTATAATTTTGAAAATACCACAAATGATATAATTTGGTTGACTGGAATGGGTAT
GGTGGCAAAGAATTCTCATGCACCATTTATTGCATCAATTGATAAGTCATTCTTTGGTGTTAAGGATTTATCAGAAATCA
CTCATATAAAAAGTTTTGAAGCTTTGCTTGAGCATCCTAGATATAAAGAGTGGAATGATTTTAGAAACCTTGATGTTGCT
GCATATATAGGTTTGACCGTAGGTGATTTTATGTTGCGGCAACCATATAATCCTGAGAATAATCCAGTTCAGTATAAACT
TATGGAAGGCTTTAATGAGTTTGTTGATTATGATAAGAATGAAAGTTATCTATGGGGACCTGCTTCAATTCATCTAGTTA
AGAATATGATGAGATCTTATGATAAAACTAGATGGTTCCAATATATAAGAGGAGTTGAGAGTGGTGGTTATGTAAAAAAC
TTGGTAGCTTGCGTATATGATAATAAAGGCATTCTAGAAACTAAGTCACCTCTAAATGTATTATTTGCTGATTATATGGA
GTTATCACTTGCAAATATTGGTTTAATACCATTTGTAAGTGAAAAAGGTACTAGTAACGCTTGTTTCTTTAGTGTAAATT
CTGCTAAAAAAGTCGAAGAATTTGTAGATGGATTTGACTCAGCAAACTCAAGATTAATTGCTAACCTTTCTTACACTATG
TGTATATCGAGAATATCTCATTATATTAAATGTGTAATTAGAGATAAGATTGGTAGTATTGTGGATGTCGAGTCGATTCA
AAAAATTCTTTCTGATTGGATATCAGAATTTGTCACCACAGTCTATCAACCAACCCCTTTAGAAATGGCGAGATATCCTT
TCAGAAACGTTTCTATCGAGGTTAAAACCATACCGGGTAAGCCTGGCTGGTATTCATGCAAAATAAATGTAATTCCCCAC
ATTCAATTTGAAGGAATGAATACTACAATGACTATAGATACTAGGCTTGAACCAGAATTATTCGGTACAAATAATAACTA
A