SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01493
Effector type	Tse
Cognate immunity protein	-
Related T6SS (Type)	T6SS00158 (Type ii)
Strain	Francisella tularensis subsp. novicida U112
Replicon	chromosome
Sequence	Protein sequence (493 a.a.); Nucleotide sequence (1479 bp)
Description	OpiB-3
Reference	[1] Eshraghi A, Kim J, Walls AC, et al. Secreted Effectors Encoded within and outside of the Francisella Pathogenicity Island Promote Intramacrophage Growth. Cell Host Microbe. 2016 Nov 9;20(5):573-583. doi: 10.1016/j.chom.2016.10.008. PMID: 27832588

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	FTN_1071 (OpiB-3)
Coordinates (Strand)	1132421..1133899 (-)
NCBI ID	118497662
RefSeq	NC_008601
Uniprot ID	UPI0000E89708
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-492	MAIDLLKLQKKLGYDVDTGGMCYGIAYMAIQAIIRDDLVTYISRIEYLEKTLSQHNNNQDAAINEIVEKINVAYEKRKNKQNLDCDEIKLLDILNWFDGVQIYHNYNNVLSGGRNYEIATNFFSANTNQNEKENRKIFVIAKELNILTKELIDDIFDKIDNSKEPIAFSLSTPDHIISIGKSSNYKPIYLINHDIHKHILNERSLYSEVFCAFKYSMRIALSIFRYSYQEENISIKYVTNNLDKDDLCNLLCLALQDGYVEAIKVYIEGISNLSGINKQQLLAAKKKNDGAPGLCLALQNGHSEAIKVYIEVISNLSGINKQELLAAKKQNGTPGLFMALQEGHAEAIKVYIEGIFNLSGINKQQLLAAKRSDGTPGLSVALYCGHAEAIKTYIEGITNLSGINKQELLAAKISDGTPGLFMALQNGHAEAIKAYIEGIFNIPGINRRELLAAKTLDGTPGLNIALQQGHSQAAKTYKETISKFMMLRNGLP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9947	-	-

Protein sequence: 493 a.a. .

                                    >EFF01493 NC_008601:c1133899-1132421 Type VI Secretion System Effector (Tse) [Francisella tularensis subsp. novicida U112]
MAIDLLKLQKKLGYDVDTGGMCYGIAYMAIQAIIRDDLVTYISRIEYLEKTLSQHNNNQDAAINEIVEKINVAYEKRKNK
QNLDCDEIKLLDILNWFDGVQIYHNYNNVLSGGRNYEIATNFFSANTNQNEKENRKIFVIAKELNILTKELIDDIFDKID
NSKEPIAFSLSTPDHIISIGKSSNYKPIYLINHDIHKHILNERSLYSEVFCAFKYSMRIALSIFRYSYQEENISIKYVTN
NLDKDDLCNLLCLALQDGYVEAIKVYIEGISNLSGINKQQLLAAKKKNDGAPGLCLALQNGHSEAIKVYIEVISNLSGIN
KQELLAAKKQNGTPGLFMALQEGHAEAIKVYIEGIFNLSGINKQQLLAAKRSDGTPGLSVALYCGHAEAIKTYIEGITNL
SGINKQELLAAKISDGTPGLFMALQNGHAEAIKAYIEGIFNIPGINRRELLAAKTLDGTPGLNIALQQGHSQAAKTYKET
ISKFMMLRNGLP*

                                

Nucleotide sequence: 1479 bp .

                                    >EFF01493 NC_008601:c1133899-1132421 Type VI Secretion System Effector (Tse) [Francisella tularensis subsp. novicida U112]
ATGGCTATTGATCTATTAAAACTACAAAAAAAACTTGGATATGATGTTGATACTGGTGGAATGTGTTACGGAATAGCTTA
TATGGCTATACAAGCTATTATAAGAGATGATTTAGTAACATATATTAGCAGAATAGAATATTTAGAAAAAACATTATCGC
AACATAACAATAATCAAGATGCTGCGATTAATGAAATTGTAGAAAAGATAAATGTAGCATATGAAAAAAGGAAGAATAAA
CAAAATTTAGATTGTGATGAAATAAAATTATTGGATATATTAAATTGGTTTGATGGAGTTCAAATATACCATAATTACAA
TAATGTATTATCTGGAGGTCGAAATTATGAGATTGCAACAAACTTTTTCTCAGCTAATACAAATCAAAATGAAAAAGAAA
ATAGAAAAATCTTTGTTATAGCTAAAGAATTAAATATATTAACAAAAGAGTTGATAGATGATATTTTTGATAAGATTGAT
AACAGTAAAGAACCTATTGCTTTTTCATTAAGCACCCCAGATCATATTATTTCGATAGGTAAAAGCTCTAATTATAAACC
GATATATTTAATCAATCATGATATTCATAAACATATATTAAATGAGAGATCGTTATACAGTGAAGTATTTTGTGCATTTA
AGTATTCGATGAGGATAGCTTTGAGCATTTTTAGATATTCATACCAAGAAGAAAATATTTCAATTAAATATGTCACTAAT
AATTTAGATAAAGATGATTTATGTAACTTATTATGTCTAGCTTTGCAAGATGGATACGTAGAAGCTATCAAAGTTTATAT
AGAAGGTATATCTAATCTTAGTGGAATTAATAAACAACAACTATTAGCTGCTAAAAAGAAAAATGATGGAGCTCCAGGAT
TATGTCTAGCTTTACAAAATGGCCACTCAGAAGCTATCAAAGTTTATATAGAGGTGATATCTAATCTTAGTGGAATTAAT
AAACAAGAATTATTAGCTGCTAAAAAACAAAATGGAACACCAGGGTTATTTATGGCTTTACAAGAAGGCCACGCAGAAGC
TATCAAAGTTTATATAGAGGGGATATTTAATCTTAGTGGAATTAATAAACAACAACTATTAGCTGCTAAAAGGAGTGATG
GAACACCAGGATTATCTGTGGCTTTGTATTGTGGCCACGCAGAAGCTATCAAAACCTATATTGAAGGTATAACTAATCTT
AGTGGAATTAACAAACAAGAACTATTAGCTGCTAAAATATCGGATGGAACACCTGGGTTATTTATGGCTTTACAAAATGG
CCACGCAGAAGCTATCAAAGCTTATATAGAGGGTATATTTAATATTCCTGGAATTAATAGACGAGAATTGTTAGCTGCTA
AAACCCTGGATGGAACACCTGGGTTGAATATAGCTTTGCAACAGGGTCATTCACAAGCTGCTAAAACTTATAAAGAAACG
ATATCTAAATTCATGATGTTAAGGAATGGATTACCATGA

                                