SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0177
Regulator type	protein
Regulatory pathway (from literatures)	Global regulator; TCS regulatory pathway
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00158 (Type ii)
Strain	Francisella tularensis subsp. novicida U112
Replicon	chromosome
Sequence	Protein sequence (136 a.a.); Nucleotide sequence (408 bp)
Description	Western immunoblot analysis of IglA in an mglA mutant and an mglB background revealed that IglA is not expressed at detectable levels in these strains.; MglA postively regulates T6SS in transcriptional level.
Reference	[1] de Bruin OM, Ludu JS, Nano FE. The Francisella pathogenicity island protein IglA localizes to the bacterial cytoplasm and is needed for intracellular growth. BMC Microbiol. 2007 Jan 17;7:1. PMID: 17233889 [2] Guina T, Radulovic D, Bahrami AJ, et al. MglA regulates Francisella tularensis subsp. novicida (Francisella novicida) response to starvation and oxidative stress. J Bacteriol. 2007 Sep;189(18):6580-6. Epub 2007 Jul 20. PMID: 17644593

External database links

Locus tag (Gene)	FTN_RS06615 (mglB)
Coordinates (Strand)	1362901..1363308 (+)
NCBI ID	489112095
RefSeq	NC_008601
Uniprot ID	Q5NFG0_FRATT, A0A0E2ZLI6_FRATU, O52735_FRANO, A0Q7F7_FRATN, A0A1S2WX07_9GAMM
KEGG ID	ftu:FTT_1276, ftn:FTN_1291, ftx:AW25_714, fty:CH70_633
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
IglA expression	↓	-
T6SS Transciption	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
SspB	PF04386.15	3.3e-19	4..100

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-135	MAMLRAYVVKATYNWLVDHGFTPYVLVDTEYEGVIVPANYIDEDKKILLDLSPQAIQDLVIDDNHISFAATFDSEPMSINIPIEAVLEVFSKETEQGMYAREFGYGININEGEDDETANPKKLGETNSDNVLSLD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9948	-	-

Protein sequence of regulator: 136 a.a. .

                                    >REG0177 NC_008601:1362901-1363308 [Francisella tularensis subsp. novicida U112]
MAMLRAYVVKATYNWLVDHGFTPYVLVDTEYEGVIVPANYIDEDKKILLDLSPQAIQDLVIDDNHISFAATFDSEPMSIN
IPIEAVLEVFSKETEQGMYAREFGYGININEGEDDETANPKKLGETNSDNVLSLD*

                                 

Nucleotide sequence of regulator: 408 bp .

                                    >REG0177 NC_008601:1362901-1363308 [Francisella tularensis subsp. novicida U112]
ATGGCTATGCTTAGAGCATATGTAGTTAAAGCTACATACAACTGGCTAGTTGATCATGGATTTACACCTTATGTTTTAGT
TGATACTGAGTATGAAGGTGTTATAGTACCAGCAAACTATATTGATGAAGATAAAAAAATACTTTTAGATTTATCTCCTC
AAGCAATACAAGATTTAGTTATAGATGATAATCATATTAGCTTTGCTGCAACGTTTGATAGTGAGCCAATGTCTATAAAT
ATTCCTATCGAGGCTGTCTTAGAAGTATTTTCTAAAGAAACAGAGCAAGGAATGTATGCTCGCGAATTTGGTTATGGAAT
TAATATCAATGAAGGCGAAGATGATGAAACTGCTAATCCTAAGAAATTAGGAGAAACTAATTCAGATAACGTTCTTTCAT
TAGATTAA

                                