Detailed information of effector
Summary
| Effector ID | EFF00203  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00158 (Type ii) |
| Strain | Francisella tularensis subsp. novicida U112 |
| Replicon | chromosome |
| Sequence | Protein sequence (165 a.a.); Nucleotide sequence (495 bp) |
| Description | Required for F. tularensis phagosomal escape, intramacrophage growth, virulence, and induction of IL1-beta expression by infected macrophages |
| Reference | [1] Bröms JE, Lavander M, Meyer L, et al. IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 2011 Sep;79(9):3683-96. doi: 10.1128/IAI.01344-10. Epub 2011 Jun 20. PMID: 21690239 [2] Dai S, Mohapatra NP, Schlesinger LS, et al. The acid phosphatase AcpA is secreted in vitro and in macrophages by Francisella spp. Infect Immun. 2012 Mar;80(3):1088-97. doi: 10.1128/IAI.06245-11. Epub 2011 Dec 19. PMID: 22184418 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | FTN_1312 (VgrG) |
| Coordinates (Strand) | 1384422..1384916 (+) |
| NCBI ID | 118497892 |
| RefSeq | NC_008601 |
| Uniprot ID | UPI00001A955A |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-164 | MSKADHIFNLEEQGLLIDIKDDSKGCTTKLESSGKITHNATESIESSADKQIIENVKDSKISITEKEILLATKKSSIMLSEDKIVIKIGNSLIILDDSNISLESATINIKSSANINIQASQNIDIKSLNNSIKADVNLNAEGLDVNIKGSVTASIKGSAATMVG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9898 | - | - |
Protein sequence: 165 a.a. .
>EFF00203 NC_008601:1384422-1384916 (Unknown) [Francisella tularensis subsp. novicida U112]
MSKADHIFNLEEQGLLIDIKDDSKGCTTKLESSGKITHNATESIESSADKQIIENVKDSKISITEKEILLATKKSSIMLS
EDKIVIKIGNSLIILDDSNISLESATINIKSSANINIQASQNIDIKSLNNSIKADVNLNAEGLDVNIKGSVTASIKGSAA
TMVG*
MSKADHIFNLEEQGLLIDIKDDSKGCTTKLESSGKITHNATESIESSADKQIIENVKDSKISITEKEILLATKKSSIMLS
EDKIVIKIGNSLIILDDSNISLESATINIKSSANINIQASQNIDIKSLNNSIKADVNLNAEGLDVNIKGSVTASIKGSAA
TMVG*
Nucleotide sequence: 495 bp .
>EFF00203 NC_008601:1384422-1384916 (Unknown) [Francisella tularensis subsp. novicida U112]
ATGTCAAAAGCAGACCATATTTTCAACTTAGAAGAGCAAGGCTTGCTCATAGATATCAAAGATGATTCAAAAGGCTGCAC
TACGAAACTAGAGTCCTCTGGAAAAATTACTCATAATGCAACTGAATCGATAGAGTCAAGCGCTGATAAACAAATAATTG
AAAATGTTAAAGACTCAAAAATATCAATAACTGAAAAAGAAATTTTGTTAGCTACTAAGAAATCAAGCATTATGCTTAGT
GAAGATAAAATTGTCATAAAAATAGGTAATTCTTTAATTATACTTGATGATTCAAATATTTCGCTAGAGTCTGCAACTAT
TAATATAAAAAGCTCTGCTAATATAAATATTCAAGCATCGCAGAACATAGATATAAAAAGTCTAAATAATAGTATCAAAG
CAGATGTAAACCTTAATGCTGAAGGATTAGATGTAAATATTAAAGGAAGTGTAACAGCAAGTATAAAAGGTTCCGCAGCA
ACAATGGTTGGATAA
ATGTCAAAAGCAGACCATATTTTCAACTTAGAAGAGCAAGGCTTGCTCATAGATATCAAAGATGATTCAAAAGGCTGCAC
TACGAAACTAGAGTCCTCTGGAAAAATTACTCATAATGCAACTGAATCGATAGAGTCAAGCGCTGATAAACAAATAATTG
AAAATGTTAAAGACTCAAAAATATCAATAACTGAAAAAGAAATTTTGTTAGCTACTAAGAAATCAAGCATTATGCTTAGT
GAAGATAAAATTGTCATAAAAATAGGTAATTCTTTAATTATACTTGATGATTCAAATATTTCGCTAGAGTCTGCAACTAT
TAATATAAAAAGCTCTGCTAATATAAATATTCAAGCATCGCAGAACATAGATATAAAAAGTCTAAATAATAGTATCAAAG
CAGATGTAAACCTTAATGCTGAAGGATTAGATGTAAATATTAAAGGAAGTGTAACAGCAAGTATAAAAGGTTCCGCAGCA
ACAATGGTTGGATAA