SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01494
Effector type	Tle
Cognate immunity protein	-
Related T6SS (Type)	T6SS00158 (Type ii)
Strain	Francisella tularensis subsp. novicida U112
Replicon	chromosome
Sequence	Protein sequence (1326 a.a.); Nucleotide sequence (3978 bp)
Description	pdpC
Reference	[1] Eshraghi A, Kim J, Walls AC, et al. Secreted Effectors Encoded within and outside of the Francisella Pathogenicity Island Promote Intramacrophage Growth. Cell Host Microbe. 2016 Nov 9;20(5):573-583. doi: 10.1016/j.chom.2016.10.008. PMID: 27832588 [2] Brodmann M, Schnider ST, Basler M. Type VI Secretion System and Its Effectors PdpC, PdpD, and OpiA Contribute to Francisella Virulence in Galleria mellonella Larvae. Infect Immun. 2021 Jun 16;89(7):e0057920. doi: 10.1128/IAI.00579-20. Epub 2021 Jun 16. PMID: 33875476

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	FTN_1319 (pdpC)
Coordinates (Strand)	1391204..1395181 (+)
NCBI ID	118497899
RefSeq	NC_008601
Uniprot ID	UPI0000E89600
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1325	MNDKYELNIYFEKIELPKTADFNMISKHDIKELRVDANLKKKIHLLQFDEDYLAYMRSLRAIHPSKIAMQKIKSIRNKEDSFIIAIFSLDKIIHKTKFISFGHKTVIFDFKKLWGLVDFVIVHTSNKTWVNHKLTSFMPSITYCNQNIIHLAYHSDFLYIYHTPEFMDDINVDRENRRELVAKIPDPYWVRADTKENKININSEEENIEKDLKKITKLKNFEISANDIFFSKAIKAAPRLQHKNKSKLFNSLAIENNEKIKRDIIDYAISNAWYKNEGLLENLMTFLDALVVRHLYLIAVYSVYEIEIGIKSVKPEYSKLLKAGLLNKDIQNQLIYDQKKISNIIWLGETFHGLDIEEAEDLCELIDQGDINPKINPINPEKLYRAYKENYGNEALSFREYKEKYNFLDNDKLREEHANKLSSILEDPKFRVLSYINAFLCSTKNYLVPYGYLGSNPLTYYNCMLETGKRRTSKEAYFADIRNKLFMVCYLPGFLSSVIADDDFIDWYSNKKENQELLKNSYLNNLYKSKQKYIKDFIDLDIIKLLKQTSSQIKPSYIPVAFRYGAFASTTALIRSNANVSNRMEFELYDSPERLHNQYSEKEEMIMPKSVNNPKDHSIDNGISLFSLNLTNEKEDGLRKVMLKVAQLYNLDFKVGISGNLDQAMTQALILGMATTKKNGDILLDEDQMLYMTYLYCIFMAHSVDHTVDEILMSANTYLLNSEEVKYPIFNIADFFARPVFGLSKDKEFKSLVEKYENSLKPNSKILKENYISRVVTLSEVYEDIYNLNCLYSSLSEGSLYNLLSTHSERHCTLLEQYSRKKKAEIGLVQDGEKIKVVNNYSGYAAINQYQRFVSLGRMYDSGAKYSSSHKKQIEKDFNLYLPDKEQINYLKYNFKDNKFDAVYKNKKSKEKNQSHIVYAKKQNTRYCYGYFNDFFVKNRITTFYKIKDKSGNYLVNLHNEKYSFATPNSDSKIYRVSPELLNNRDDFKRVSKDIIKSYKYISFDKQKEDIVKNFGKNLYHTNYEIWVGLSHQAISCFSVLDNIDTQEAANTFIDALYYVRLMQLYYGKTIPFLWISSEIVRYSSSETHYYIPTEENFENILKIALNNASKPVIERFLIILNIYNSTIDNNALILIRCRLPIILFEYEEQKLKLKALQKCADSYKKNNYKNEINFSAWFESIFHVQNLSLSPNYIGNNILILSLVEELKPKCSEYKKNMINQQITAKANELNFYQLLIRLIEISTYHRILTKSIITNTTSLLYDILNKPEFQTINKLINKLFIYKNKDLNTDKYKAFHTKLITIESTYKKINSLYKSDFFKKISS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9972	-	-

Protein sequence: 1326 a.a. .

                                    >EFF01494 NC_008601:1391204-1395181 Type VI Lipase Effector (Tle) [Francisella tularensis subsp. novicida U112]
MNDKYELNIYFEKIELPKTADFNMISKHDIKELRVDANLKKKIHLLQFDEDYLAYMRSLRAIHPSKIAMQKIKSIRNKED
SFIIAIFSLDKIIHKTKFISFGHKTVIFDFKKLWGLVDFVIVHTSNKTWVNHKLTSFMPSITYCNQNIIHLAYHSDFLYI
YHTPEFMDDINVDRENRRELVAKIPDPYWVRADTKENKININSEEENIEKDLKKITKLKNFEISANDIFFSKAIKAAPRL
QHKNKSKLFNSLAIENNEKIKRDIIDYAISNAWYKNEGLLENLMTFLDALVVRHLYLIAVYSVYEIEIGIKSVKPEYSKL
LKAGLLNKDIQNQLIYDQKKISNIIWLGETFHGLDIEEAEDLCELIDQGDINPKINPINPEKLYRAYKENYGNEALSFRE
YKEKYNFLDNDKLREEHANKLSSILEDPKFRVLSYINAFLCSTKNYLVPYGYLGSNPLTYYNCMLETGKRRTSKEAYFAD
IRNKLFMVCYLPGFLSSVIADDDFIDWYSNKKENQELLKNSYLNNLYKSKQKYIKDFIDLDIIKLLKQTSSQIKPSYIPV
AFRYGAFASTTALIRSNANVSNRMEFELYDSPERLHNQYSEKEEMIMPKSVNNPKDHSIDNGISLFSLNLTNEKEDGLRK
VMLKVAQLYNLDFKVGISGNLDQAMTQALILGMATTKKNGDILLDEDQMLYMTYLYCIFMAHSVDHTVDEILMSANTYLL
NSEEVKYPIFNIADFFARPVFGLSKDKEFKSLVEKYENSLKPNSKILKENYISRVVTLSEVYEDIYNLNCLYSSLSEGSL
YNLLSTHSERHCTLLEQYSRKKKAEIGLVQDGEKIKVVNNYSGYAAINQYQRFVSLGRMYDSGAKYSSSHKKQIEKDFNL
YLPDKEQINYLKYNFKDNKFDAVYKNKKSKEKNQSHIVYAKKQNTRYCYGYFNDFFVKNRITTFYKIKDKSGNYLVNLHN
EKYSFATPNSDSKIYRVSPELLNNRDDFKRVSKDIIKSYKYISFDKQKEDIVKNFGKNLYHTNYEIWVGLSHQAISCFSV
LDNIDTQEAANTFIDALYYVRLMQLYYGKTIPFLWISSEIVRYSSSETHYYIPTEENFENILKIALNNASKPVIERFLII
LNIYNSTIDNNALILIRCRLPIILFEYEEQKLKLKALQKCADSYKKNNYKNEINFSAWFESIFHVQNLSLSPNYIGNNIL
ILSLVEELKPKCSEYKKNMINQQITAKANELNFYQLLIRLIEISTYHRILTKSIITNTTSLLYDILNKPEFQTINKLINK
LFIYKNKDLNTDKYKAFHTKLITIESTYKKINSLYKSDFFKKISS*

                                

Nucleotide sequence: 3978 bp .

                                    >EFF01494 NC_008601:1391204-1395181 Type VI Lipase Effector (Tle) [Francisella tularensis subsp. novicida U112]
ATGAACGACAAATATGAACTAAATATCTATTTTGAGAAAATAGAGTTACCTAAAACAGCAGATTTCAATATGATAAGCAA
GCATGATATTAAAGAACTCAGAGTAGATGCTAATTTAAAAAAGAAAATACACTTATTACAATTTGATGAAGATTATTTAG
CATATATGAGGAGCTTAAGAGCTATTCATCCCTCAAAAATAGCAATGCAAAAAATTAAATCAATTCGCAATAAAGAAGAT
TCTTTTATAATAGCCATTTTTTCTTTAGATAAAATAATTCACAAAACTAAATTTATCTCTTTTGGACATAAAACCGTTAT
TTTTGATTTTAAAAAACTATGGGGATTAGTTGATTTCGTAATAGTTCATACAAGTAATAAAACCTGGGTTAATCATAAGT
TAACATCATTTATGCCATCAATTACATACTGTAATCAAAACATCATCCATTTAGCATACCATTCTGATTTTTTATATATT
TATCATACTCCAGAATTTATGGATGATATAAATGTAGATCGAGAAAATAGAAGAGAGTTAGTGGCAAAAATTCCCGATCC
ATATTGGGTAAGAGCTGATACTAAAGAAAATAAAATCAATATTAATTCTGAAGAGGAAAATATAGAAAAAGATTTAAAAA
AAATAACTAAATTAAAAAATTTTGAAATAAGCGCAAATGATATTTTTTTCTCAAAAGCTATAAAAGCAGCTCCAAGATTA
CAACATAAAAATAAGAGTAAATTATTTAATTCATTAGCGATTGAAAATAATGAAAAAATCAAACGTGACATAATTGATTA
TGCAATATCTAATGCTTGGTATAAAAACGAGGGGTTACTTGAAAATCTAATGACATTTTTAGACGCGCTAGTCGTAAGAC
ATCTCTACTTAATAGCAGTTTACTCAGTTTATGAAATAGAAATAGGTATTAAATCAGTAAAACCTGAGTATAGCAAATTA
TTAAAAGCTGGCCTATTGAACAAAGATATTCAAAATCAATTGATATATGATCAAAAGAAAATTAGCAATATTATTTGGCT
TGGTGAAACATTTCATGGTTTAGATATTGAAGAAGCAGAAGATTTATGTGAACTTATTGACCAAGGCGATATTAATCCAA
AAATTAATCCTATAAATCCTGAAAAGCTATATAGAGCCTATAAAGAAAATTATGGAAATGAAGCGTTAAGCTTTAGAGAA
TACAAAGAAAAATATAATTTTTTAGATAATGATAAGCTTAGAGAAGAACATGCAAATAAACTTTCAAGCATATTAGAAGA
TCCAAAATTTAGAGTTCTTAGCTATATAAATGCATTTTTATGTTCAACTAAAAATTATCTAGTACCTTATGGGTATTTAG
GAAGTAATCCTCTTACATACTATAATTGTATGCTAGAAACCGGAAAACGTAGAACATCAAAAGAAGCATATTTTGCTGAT
ATAAGAAACAAATTGTTTATGGTATGCTATCTTCCTGGCTTCTTGAGCTCTGTAATTGCTGATGATGACTTTATAGACTG
GTATTCTAATAAGAAAGAAAATCAAGAATTACTCAAAAACTCTTATTTGAACAATTTATATAAGTCAAAGCAGAAATATA
TAAAAGATTTTATAGATCTTGATATTATTAAACTTCTAAAACAGACATCATCTCAAATCAAGCCAAGCTATATACCAGTA
GCTTTTAGATATGGTGCATTTGCCTCAACAACTGCTTTGATAAGATCAAATGCAAATGTTTCTAATCGAATGGAGTTTGA
ATTATATGACTCACCTGAAAGACTACATAATCAATACTCAGAAAAAGAGGAAATGATTATGCCAAAATCGGTTAATAACC
CCAAGGATCACTCAATTGACAATGGAATATCTTTATTTAGCCTTAATCTAACAAATGAAAAAGAAGATGGACTTCGAAAA
GTTATGTTGAAAGTTGCACAACTATATAATCTTGATTTCAAGGTAGGTATCTCAGGAAATTTAGATCAAGCAATGACTCA
GGCATTAATACTTGGGATGGCAACTACAAAAAAAAATGGCGATATCTTACTTGATGAAGATCAAATGCTATATATGACAT
ACTTATACTGCATTTTCATGGCACATAGTGTCGATCATACTGTAGATGAAATACTAATGTCTGCAAATACTTATCTGTTA
AATTCTGAAGAGGTAAAGTATCCTATATTTAATATAGCTGATTTTTTTGCTAGGCCTGTATTTGGACTCTCTAAGGATAA
AGAGTTTAAATCATTAGTTGAAAAGTATGAAAATAGCCTAAAACCTAATTCAAAAATTCTCAAAGAGAATTATATAAGTA
GAGTAGTCACACTATCTGAAGTATATGAAGATATATATAACTTAAATTGTTTATATAGTTCTCTCTCAGAAGGATCCTTG
TATAATCTACTATCAACTCACAGTGAAAGACACTGTACTTTATTAGAGCAGTATTCTCGCAAGAAGAAAGCTGAGATAGG
ATTAGTCCAAGATGGAGAGAAAATAAAAGTCGTAAACAACTACAGTGGCTATGCTGCGATTAATCAATATCAACGTTTTG
TATCACTAGGCAGAATGTATGATAGTGGTGCAAAATACTCCTCTTCTCATAAAAAACAGATAGAAAAAGATTTCAACTTA
TACTTACCAGATAAAGAACAGATAAATTATCTAAAATATAACTTTAAAGATAATAAATTTGATGCTGTTTATAAAAACAA
AAAGAGCAAAGAAAAAAATCAAAGCCATATTGTGTATGCAAAAAAACAAAATACGCGATATTGCTACGGATATTTTAACG
ATTTTTTCGTTAAAAATAGAATTACGACTTTTTATAAAATAAAAGATAAATCAGGAAACTATTTAGTAAACTTACATAAT
GAAAAATATAGTTTCGCAACACCTAATTCAGATTCTAAAATATATAGAGTTTCACCAGAATTACTTAACAATAGAGATGA
TTTTAAAAGAGTATCTAAAGATATTATAAAATCATATAAATATATTAGTTTTGATAAACAAAAGGAAGATATAGTTAAAA
ATTTTGGTAAAAACTTATATCATACTAATTATGAGATATGGGTTGGGCTATCACATCAAGCTATATCATGTTTTTCAGTT
TTAGATAACATAGATACACAAGAAGCTGCTAATACTTTTATCGATGCTTTATATTATGTTAGATTAATGCAATTATATTA
TGGCAAAACTATACCTTTTTTGTGGATTAGTAGCGAAATTGTAAGATATTCTTCATCAGAAACTCACTACTATATACCAA
CAGAAGAAAACTTTGAAAATATACTTAAAATTGCTCTTAATAATGCCTCAAAACCAGTAATAGAAAGATTCTTAATTATT
CTTAACATATATAATAGTACTATTGATAATAATGCTTTAATTTTGATACGATGCCGTTTACCAATAATATTATTTGAATA
TGAAGAGCAGAAACTTAAATTAAAAGCTTTGCAAAAGTGTGCTGATTCTTATAAAAAAAATAATTATAAAAATGAAATTA
ACTTTAGTGCTTGGTTTGAATCTATATTTCATGTTCAAAACTTATCCTTATCTCCTAATTATATTGGCAATAATATTTTA
ATCTTGAGTTTAGTTGAAGAATTAAAACCAAAATGTAGTGAATATAAAAAAAACATGATTAACCAACAAATTACAGCTAA
AGCAAACGAGTTAAACTTTTATCAACTTCTAATAAGACTTATTGAAATATCAACCTATCATAGAATACTTACGAAATCAA
TCATAACTAATACAACCTCGTTACTTTATGACATATTAAATAAACCAGAATTTCAAACAATAAATAAATTAATAAATAAG
CTATTTATTTATAAAAACAAGGATCTAAATACTGATAAATATAAAGCCTTTCACACTAAGCTTATAACTATTGAAAGTAC
CTATAAAAAAATAAATAGCCTATATAAATCAGACTTTTTTAAAAAAATATCGTCATAG

                                