Detailed information of regulator
Summary
| Regulator ID | REG0173  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00158 (Type ii) |
| Strain | Francisella tularensis subsp. novicida U112 |
| Replicon | chromosome |
| Sequence | Protein sequence (206 a.a.); Nucleotide sequence (618 bp) |
| Description | RT-PCR analysis show MglA is a positive transcriptional regulator of the iglC operon.; PmrA, MglA, and SspA are parts of the same protein complex. |
| Reference | [1] Baron GS, Nano FE. MglA and MglB are required for the intramacrophage growth of Francisella novicida. Mol Microbiol. 1998 Jul;29(1):247-59. PMID: 9701818 [2] Bell BL, Mohapatra NP, Gunn JS. Regulation of virulence gene transcripts by the Francisella novicida orphan response regulator PmrA: role of phosphorylation and evidence of MglA/SspA interaction. Infect Immun. 2010 May;78(5):2189-98. doi: 10.1128/IAI.00021-10. Epub 2010 Mar 15. PMID: 20231408 |
External database links
| Locus tag (Gene) | FTN_RS06610 (mglA) |
| Coordinates (Strand) | 1362282..1362899 (+) |
| NCBI ID | 489124662 |
| RefSeq | NC_008601 |
| Uniprot ID | O52734_FRANO, A0Q7F6_FRATN, A0A1S2WVL9_9GAMM |
| KEGG ID | fty:CH70_635, ftn:FTN_1290, ftx:AW25_716 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| iglC transcription | ↓ | - |
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GST_N_3 | PF13417.8 | 2.1e-06 | 11..78 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-205 | MLLYTKKDDIYSDIVRMILLIKGANAKIVDVSKEENSKHLEELNIITPNGNIPTLSTDDFAVYRLSVIIEAIEDLYPFPPMFPVFPKQRANARILLEYVNKTFLQNIIKLQSPDLDEKQANEIKMLMQRDIISTYKTIVSEREVNAESNPDAQNINVLTLIITFVFYYFIKLKISIPTKDKNIIKEIKELLSEPNFIKTIKAKGA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9813 | - | - |
Protein sequence of regulator: 206 a.a. .
>REG0173 NC_008601:1362282-1362899 [Francisella tularensis subsp. novicida U112]
MLLYTKKDDIYSDIVRMILLIKGANAKIVDVSKEENSKHLEELNIITPNGNIPTLSTDDFAVYRLSVIIEAIEDLYPFPP
MFPVFPKQRANARILLEYVNKTFLQNIIKLQSPDLDEKQANEIKMLMQRDIISTYKTIVSEREVNAESNPDAQNINVLTL
IITFVFYYFIKLKISIPTKDKNIIKEIKELLSEPNFIKTIKAKGA*
MLLYTKKDDIYSDIVRMILLIKGANAKIVDVSKEENSKHLEELNIITPNGNIPTLSTDDFAVYRLSVIIEAIEDLYPFPP
MFPVFPKQRANARILLEYVNKTFLQNIIKLQSPDLDEKQANEIKMLMQRDIISTYKTIVSEREVNAESNPDAQNINVLTL
IITFVFYYFIKLKISIPTKDKNIIKEIKELLSEPNFIKTIKAKGA*
Nucleotide sequence of regulator: 618 bp .
>REG0173 NC_008601:1362282-1362899 [Francisella tularensis subsp. novicida U112]
TTGCTTTTATACACAAAAAAAGATGATATCTATAGCGATATAGTCCGCATGATCCTTCTTATTAAAGGGGCTAATGCGAA
AATTGTAGATGTTTCTAAAGAAGAAAACTCAAAACATCTAGAAGAGCTAAATATCATTACACCTAATGGTAATATACCTA
CGCTTAGCACAGATGATTTTGCAGTGTATAGGCTTAGTGTGATTATAGAAGCTATAGAGGATCTATATCCCTTTCCTCCG
ATGTTTCCAGTATTTCCAAAACAGCGAGCTAATGCAAGAATATTGTTAGAATATGTTAATAAGACGTTTCTACAAAATAT
TATTAAATTACAAAGTCCTGATTTGGATGAAAAACAAGCTAACGAAATAAAAATGCTAATGCAAAGGGATATAATAAGCA
CTTATAAGACAATAGTTAGTGAAAGAGAAGTAAATGCAGAAAGTAATCCAGATGCTCAAAATATCAACGTATTGACTCTG
ATAATAACTTTCGTTTTTTATTATTTCATTAAGTTAAAGATCTCAATACCTACCAAAGATAAAAACATTATCAAAGAGAT
CAAAGAATTACTTAGCGAACCTAACTTTATAAAAACTATCAAAGCAAAAGGAGCTTAA
TTGCTTTTATACACAAAAAAAGATGATATCTATAGCGATATAGTCCGCATGATCCTTCTTATTAAAGGGGCTAATGCGAA
AATTGTAGATGTTTCTAAAGAAGAAAACTCAAAACATCTAGAAGAGCTAAATATCATTACACCTAATGGTAATATACCTA
CGCTTAGCACAGATGATTTTGCAGTGTATAGGCTTAGTGTGATTATAGAAGCTATAGAGGATCTATATCCCTTTCCTCCG
ATGTTTCCAGTATTTCCAAAACAGCGAGCTAATGCAAGAATATTGTTAGAATATGTTAATAAGACGTTTCTACAAAATAT
TATTAAATTACAAAGTCCTGATTTGGATGAAAAACAAGCTAACGAAATAAAAATGCTAATGCAAAGGGATATAATAAGCA
CTTATAAGACAATAGTTAGTGAAAGAGAAGTAAATGCAGAAAGTAATCCAGATGCTCAAAATATCAACGTATTGACTCTG
ATAATAACTTTCGTTTTTTATTATTTCATTAAGTTAAAGATCTCAATACCTACCAAAGATAAAAACATTATCAAAGAGAT
CAAAGAATTACTTAGCGAACCTAACTTTATAAAAACTATCAAAGCAAAAGGAGCTTAA