SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01487
Effector type	Tse
Cognate immunity protein	-
Related T6SS (Type)	T6SS00158 (Type ii)
Strain	Francisella tularensis subsp. novicida U112
Replicon	chromosome
Sequence	Protein sequence (436 a.a.); Nucleotide sequence (1308 bp)
Description	PI3K activity of OpiA can play an important role in the pathogenesis of F. novicida
Reference	[1] Eshraghi A, Kim J, Walls AC, et al. Secreted Effectors Encoded within and outside of the Francisella Pathogenicity Island Promote Intramacrophage Growth. Cell Host Microbe. 2016 Nov 9;20(5):573-583. doi: 10.1016/j.chom.2016.10.008. PMID: 27832588 [2] Ledvina HE, Kelly KA, Eshraghi A, et al. A Phosphatidylinositol 3-Kinase Effector Alters Phagosomal Maturation to Promote Intracellular Growth of Francisella. Cell Host Microbe. 2018 Aug 8;24(2):285-295.e8. doi: 10.1016/j.chom.2018.07.003. Epub 2018 Jul 26. PMID: 30057173 [3] Brodmann M, Schnider ST, Basler M. Type VI Secretion System and Its Effectors PdpC, PdpD, and OpiA Contribute to Francisella Virulence in Galleria mellonella Larvae. Infect Immun. 2021 Jun 16;89(7):e0057920. doi: 10.1128/IAI.00579-20. Epub 2021 Jun 16. PMID: 33875476

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	FTN_0131 (opiA)
Coordinates (Strand)	143587..144894 (-)
NCBI ID	118496746
RefSeq	NC_008601
Uniprot ID	UPI0000E89733
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-435	MKNFEVIRKDFLQSIEIYINHPYKSHFLSIHHRHRDTGIRRAALLKDYILHCPNLTIMNQLIKHYLERSQELEEVLLNLKLQYKKTITKAEIKVDASLLRGGNKHPSSLWTYVNNVFQQTKNQAPIENNLSIINNPIFDVFLPVQNISKIGNSTASAHVVTRYKDKKNEKVNYFVKSLNNNEVENAIAEVIYAQIWNYFIGNRASKSLLLLSEHENKIIGIASKGISDFQEYKSLSGDQKDYPGLIQILFYVCVLIENDFHICNFGTGLFNGKRCYSKIDHDYIVSFWDALKYGKFMTEVSHNFKELFKKKSMSSLFLLLESMRFSPVKANSRILELGHKIRLGRPSTVTSHMMMSQFRNKAITRSNQKELEYTINDFILKTRPAEISRFFSDLEGVLMTKIAPLIQYNYKYLGKSNELLFFFKLRFSHLCNLLN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9979	-	-

Protein sequence: 436 a.a. .

                                    >EFF01487 NC_008601:c144894-143587 Type VI Secretion System Effector (Tse) [Francisella tularensis subsp. novicida U112]
MKNFEVIRKDFLQSIEIYINHPYKSHFLSIHHRHRDTGIRRAALLKDYILHCPNLTIMNQLIKHYLERSQELEEVLLNLK
LQYKKTITKAEIKVDASLLRGGNKHPSSLWTYVNNVFQQTKNQAPIENNLSIINNPIFDVFLPVQNISKIGNSTASAHVV
TRYKDKKNEKVNYFVKSLNNNEVENAIAEVIYAQIWNYFIGNRASKSLLLLSEHENKIIGIASKGISDFQEYKSLSGDQK
DYPGLIQILFYVCVLIENDFHICNFGTGLFNGKRCYSKIDHDYIVSFWDALKYGKFMTEVSHNFKELFKKKSMSSLFLLL
ESMRFSPVKANSRILELGHKIRLGRPSTVTSHMMMSQFRNKAITRSNQKELEYTINDFILKTRPAEISRFFSDLEGVLMT
KIAPLIQYNYKYLGKSNELLFFFKLRFSHLCNLLN*

                                

Nucleotide sequence: 1308 bp .

                                    >EFF01487 NC_008601:c144894-143587 Type VI Secretion System Effector (Tse) [Francisella tularensis subsp. novicida U112]
ATGAAAAATTTTGAAGTAATACGCAAAGATTTTCTACAGTCAATAGAAATATATATCAATCATCCATATAAGAGCCACTT
TTTAAGTATACATCACCGGCATAGGGATACCGGCATTCGAAGAGCCGCTCTATTAAAAGATTATATCTTACATTGCCCTA
ACTTAACCATAATGAATCAATTAATCAAACATTATTTAGAAAGGTCCCAAGAGCTAGAAGAGGTTCTTTTAAACCTTAAA
TTACAATATAAAAAAACAATCACTAAAGCAGAAATAAAAGTTGATGCTTCATTACTAAGAGGAGGAAATAAGCATCCAAG
TTCTTTGTGGACTTATGTTAATAATGTTTTCCAACAAACTAAGAATCAAGCTCCCATAGAAAACAATTTATCTATTATAA
ATAATCCTATTTTCGATGTTTTCTTACCTGTTCAAAATATTTCCAAAATAGGAAATTCTACTGCATCTGCACATGTAGTG
ACTCGCTATAAAGATAAAAAGAATGAAAAAGTAAACTATTTTGTTAAATCTCTTAATAATAATGAAGTTGAAAATGCAAT
TGCTGAAGTTATCTATGCTCAAATTTGGAATTATTTTATTGGAAATCGCGCTTCAAAATCTCTATTATTATTATCTGAGC
ATGAAAACAAAATTATTGGCATTGCTTCAAAAGGCATAAGTGATTTTCAAGAATATAAATCACTTAGTGGGGATCAAAAA
GATTACCCTGGACTAATACAAATTTTATTTTATGTTTGTGTGTTAATAGAAAATGATTTTCATATCTGTAATTTTGGTAC
AGGACTATTTAATGGAAAGCGGTGTTATAGTAAGATAGATCACGACTATATTGTGAGTTTTTGGGATGCTTTAAAATATG
GGAAATTTATGACAGAGGTCTCGCACAATTTTAAAGAATTATTTAAAAAAAAATCTATGTCTAGTTTATTTCTTCTTTTA
GAATCAATGCGTTTTAGTCCTGTTAAAGCAAATTCAAGAATCTTAGAATTAGGTCATAAAATACGTCTTGGAAGACCGTC
AACAGTTACTTCACATATGATGATGAGTCAATTTCGAAATAAGGCAATAACCAGGAGTAATCAAAAAGAACTTGAATATA
CAATAAATGATTTTATTCTTAAAACTAGACCTGCTGAAATTAGTAGATTTTTTTCAGATCTTGAAGGAGTTTTAATGACT
AAAATCGCTCCCCTTATTCAGTATAACTATAAATACTTAGGTAAATCCAATGAGTTATTATTCTTTTTCAAATTAAGATT
TTCTCATTTGTGTAATTTGTTGAATTAA

                                